Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Cell Dissociation Buffers
Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Phosphate Buffered Saline (PBS). Gibco® Cell Dissociation Buffer is suitable for gentle dissociation of mammalian cells for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines such as HeLa and NIH 3T3, but is not recommended for routine passage of strongly adherent cells. The dis...
Catalog: 13151-014
Size: 100 ml
Category: Products & Services > Kits & Assays > Enzyme Detection & Activity Assays > Phosphatase Assay Kits
The RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensur...
Catalog: R33700
Size: 1 kit
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
PKN1 (PRK1) belongs to the protein kinase C superfamily. This kinase is activated by the Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can also be activated by phospholipids.
Catalog: PV3790
Size: 10 µg
Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Chromogens
TMB (Tetramethyl Benzidine) chromogen solution may be used as a substrate for horseradish peroxidase (HRP) in ELISAs.
Catalog: 00-2023
Size: 500 ml
Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Chemiluminescent Substrates
This concentrated Nitro-Block-II™ Enhancer can be used with CSPD® or CDP-Star® chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II™ membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF membranes. • Chemically optimized formulations provide enhanced signal intensity in blotting applications with dioxetane-based substrate solutions (CSPD® or CDP-Star® substrates) on nitrocellulose or PVDF membranes. • Enhancer increases signal intensity, allowing decreased signal acquisition time. Increa...
Catalog: T2184
Size: 20 ml
Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Chromogens
Stable DAB is a stable, pre-diluted chromogen system designed to clearly localize tissue, DNA, mRNA, and antigens in in situ hybridization and immunoperoxidase procedures. Stable DAB is a ready-to-use reagent containing the correct amounts of diaminobenzidine and hydrogen peroxide. Stable DAB does not fade, creating a permanent record of results under a standard coverslip. It is color coded red for easy user identification.
Catalog: 750118
Size: 250 ml
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
BMPR1A is a Type I member of the TGF beta receptor superfamily of transmembrane ser⁄thr kinases which phosphorylates intracellular SMADs in response to bone morphogenic proteins.
Catalog: PV6038
Size: 10 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
MAP3K14 (NIK) is structurally related to MAP3Ks. MAP3K14 phosphorylates and activates CHUK (IKKalpha) and IKBKB (IKK beta), which in turn phosphorylate IkB-alpha, releasing NF-KappaB leading to up-regulation of inflammatory response genes.
Catalog: PV4902
Size: 10 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
MAPK9 (JNK2) is a serine⁄threonine kinase which plays an important role in the MAPK pathway.
Catalog: PV4018
Size: 1 mg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
HIV-1 glycoprotein 120 (gp120, fragment of env), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the HIV-1 surface glycoprotein gp120 (Ala 29-Glu 503), which is cleaved from the full length envelope glycoprotein gp160, fused to a polyhistidine tag at the C-terminus and a si...
Catalog: 11233-V08H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
HIV-1 glycoprotein 120 (gp120, fragment of env), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the HIV-1 surface glycoprotein gp120 (Ala 29-Glu 503), which is cleaved from the full length envelope glycoprotein gp160, fused to a polyhistidine tag at the C-terminus and a si...
Catalog: 11233-V08H-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ephrin-B1 (EFNB1), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 232) of human EFNB1 (NP_004420.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized mouse EFNB3 at 2 µg/ml (100 µl/well) can bind human EFNB1-Fc wit...
Catalog: 10894-H03H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ephrin-B1 (EFNB1), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 232) of human EFNB1 (NP_004420.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized mouse EFNB3 at 2 µg/ml (100 µl/well) can bind human EFNB1-Fc wit...
Catalog: 10894-H03H-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ephrin-B2 (EFNB2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ala 229) of human EFNB2 (NP_004084.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized human EFNB4-His at 2 µg/ml (100 µl/well...
Catalog: 10881-H03H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Other Proteins & Peptides
Ephrin-B2 (EFNB2), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ala 229) of human EFNB2 (NP_004084.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Activity: Measured by its binding ability in a functional ELISA . Immobilized human EFNB4-His at 2 µg/ml (100 µl/well...
Catalog: 10881-H03H-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Fc gamma receptor III-a (FCGR3A/CD16a), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1- Gln 208) of human FCGR3A (P08637-1) fused to a polyhistidine tag at the C-terminus. Activity: Using the Octet RED System, the affinity constant (Kd) of CD16a bound to Human IgG1 was 180nM. Formulat...
Catalog: 10389-H08H-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Viral & Microbial Proteins
Hemagglutinin, Influenza A virus (HA), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the native extracellular domain (Met 8-Phe 544) of influenza A virus, subtype H7N7 (A/Netherlands/219/03), HA (AAR02640.1) (Met 8-Phe 544), fused to a polyhistidine tag at the C-terminus. Activity: Measured ...
Catalog: 11082-V08B-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Viral & Microbial Proteins
Hemagglutinin, Influenza A virus (HA), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the native extracellular domain (Met 8-Phe 544) of influenza A virus, subtype H7N7 (A/Netherlands/219/03), HA (AAR02640.1) (Met 8-Phe 544), fused to a polyhistidine tag at the C-terminus. Activity: Measured ...
Catalog: 11082-V08B-50
Size: 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Cluster of Differentiation 27 (CD27), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ile 192) of human CD27 (NP_001233.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the carboxy-terminus. Activity: Measured by its ability to bind to recombinant human CD70 . Immobilized recombinant human CD27 at 2 µg/ml (100 µl/well) c...
Catalog: 10039-H03H-25
Size: 25 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > CD & Other Cell Surface Markers
Cluster of Differentiation 27 (CD27), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ile 192) of human CD27 (NP_001233.1) fused to the polyhistidine-tagged Fc region of human IgG1 at the carboxy-terminus. Activity: Measured by its ability to bind to recombinant human CD70 . Immobilized recombinant human CD27 at 2 µg/ml (100 µl/well) c...
Catalog: 10039-H03H-5
Size: 5 µg