Gibco® MEM Amino Acids Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Amino Acids Solution is formulated to contain 50X the essential amino acids (except L-glutamine) found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Amino Acids Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufactur...
Size: 100 ml
Clone/PAD: PY20 Immunogen: Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine Specificity: Reacts specifically with tyrosine phosphorylated proteins in the native or denatured states, and does not react with phosphoserine or phosphothreonine. Reacts with a wide variety of tyrosine-phosphorylated proteins in all tested species. Reactivity: Human, Mouse, (positive controls: mouse brain, NIH 3T3 cells (+/- TPA), K562 cells, EGF-stimulated A431 cells, and MCF-7 cell lysates) Applications: Western blotting, Immunoprecipitation, ELISA, Affinity chromotograp...
Size: 100 µg
The cell-permeant SYTO 82 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.
Size: 250 µl
The cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.
Size: 250 µl
SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population. • Impermeant to live cells • Excitation/Emission: 504/523 nm • Use with 488 Argon-ion laser • Works with mammalian cells and both Gram-positive and Gram-negative bacteria Simple Viability Determination SYTOX® Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, ...
Size: 250 µl
The Ambion® RecoverAll™ Total Nucleic Acid Isolation Kit is for the extraction of total nucleic acid from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. Sufficient reagents are included for 40 purifications from up to four 20 µm sections, or up to 35 mg of unsectioned core samples each. Features of the RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE: • Optimized for isolation of total nucleic acids, including microRNAs, from FFPE tissue • No overnight Proteinase K digestion required—deparaffinize in the morning and perform qRT-PCR in the afternoon • Typical yields are...
Size: 40 purifications
Our 4-acetamido-4'-maleimidylstilbene-2,2'- disulfonic acid is a thiol-reactive reagent that is water soluble, with high polarity and membrane impermeability. This polysulfonated dye is useful for determining whether thiol-containing proteins and polypeptide chains are exposed at the extracellular or cytoplasmic membrane surface.
Size: 25 mg
Intracellular calibration of the fluorescence response of cytosolic pH indicators is typically performed using the K + /H + ionophore nigericin , which causes equilibration of intracellular and extracellular pH in the presence of a depolarizing concentration of extracellular K + .
Size: 10 mg
DTNB or Elman's reagent can be used to quantitate thiols in proteins, cells and plasma by absorption measurements. It readily forms a mixed disulfide with thiols, liberating the chromophore 5-merapto-2-nitrobenzoic acid (absorption maximum 410 nm). Only protein thiols that are accessible to this water-soluble reagent are modified.
Size: 10 g
DAF-FM is a reagent that is used to detect and quantify low concentrations of nitric oxide (NO). It is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and fluorometers. DAF-FM Specifications: • Ex/Em of DAF-FM: ~495/515 nm • Lyophilized product should be dissolved using DMSO and then added to an aqueous buffer to create a working solution • DAF-FM diacetate is cell permeant and passively diffuses across...
Size: 10 × 50 µg
Tetrafluorophenyl (TFP) esters are an improvement over the succinimidyl ester (SE or NHS-ester) chemistry typically used to attach fluorophores or haptens to the primary amines of biomolecules. Both reactive chemistries produce the same strong amide bond between the dye or hapten and the compound of interest, but TFP esters are less susceptible to spontaneous hydrolysis during conjugation reactions. Alexa Fluor TFP esters are stable for several hours at the basic pH typically used for reactions – far outlasting succinimidyl esters.
Size: 1 mg
Acid Phenol:Chloroform:IAA (125:24:1) is premixed and supplied at pH 4.5 ± 0.2. Provided in one bottle of 400 mL. In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when mixing. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality-tested, saturated phenols are ready to use, eliminating handling problems and offering a more convenient, safer, an...
Size: 400 ml
Custom TaqMan® SNP Genotyping Assay, Non-Human LG
Size: 750 µl
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Size: 5 mg
Acid Phenol:Chloroform:IAA (125:24:1) is premixed and supplied at pH 4.5 ± 0.2. Provided in one bottle of 100 mL. In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when mixing. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more convenient, safer, an...
Size: 100 ml
DNDS is a stilbene sulfonate that can be used to inhipit anionic transport.
Size: 1 g
The amine-reactive Alexa Fluor 430 carboxylic acid, succinimidyl ester and its conjugates exhibit the rare combination of absorption between 400 nm and 450 nm and fluorescence emission beyond 500 nm (excitation/emission maxima ~434/539 nm).
Size: 5 mg
Ambion® Non-Stick RNase-free Microfuge Tubes have a low binding surface and are guaranteed RNase- and DNase-free. They have a positive seal design that withstands –90° to 120°C and are marked with convenient graduations. 1.5 mL tubes are provided in a box of 250. Each lot of tubes is certified RNAse- and DNase-free.
Size: 250 tubes
This single 5-isomer preparation of our proprietary, amine-reactive dye, Oregon Green 488 carboxylic acid, succinimidyl ester and its conjugates have green fluorescence similar to that of fluorescein but are more photostable. Additionally, Oregon Green 488 dye has a lower pK a than fluorescein (pK a = 4.7 versus 6.4 for fluorescein), making this bright dye less pH sensitive in the physiological range.
Size: 5 mg
To permit simultaneous long-term tracing of mixed-cell populations using different fluorescent colors, we have developed a probe whose applications should be similar to those of CFDA SE (commonly called CFSE). Cells labeled with our SNARF-1 carboxylic acid, acetate, succinimidyl ester have orange-red fluorescence that can be easily distinguished from that of cells loaded with green-fluorescent tracers such as CFDA SE.
Size: 10 × 50 µg