Oligo Synthesis Reagents

6-FAM™ Dye Phosphoramidite, 45 mg

Category: Products & Services > Buffers & Chemicals > General Chemicals > Oligo Synthesis Reagents

Contains 45 mg 6-FAM™ Dye Phosphoramidite. 6-FAM™ Dye Phosphoramidite is used for the 5’ attachment of FAM™ dye during oligonucleotide synthesis. 6-FAM™ Dye Phosphoramidite is an optically pure isomer (not a mixture). The label is treated as if it were simply another base: It is inserted at the appropriate place in the sequence and incorporated automatically. • Specialty phosphoramidites allow online addition of the label during synthesis, saving time in production of labeled probes and primers • Designed and optimized for use with ABI™ synthesizers, guaranteeing reliable nucleic acid synthesi...

Catalog: 403169
Size: 45 mg

TET™ Dye Phosphoramidite, 50 mg

Category: Products & Services > Buffers & Chemicals > General Chemicals > Oligo Synthesis Reagents

Contains 50 mg TET™ Dye Phosphoramidite. TET™ Dye Phosphoramidite is used for the 5’ attachment of TET™ dye during oligonucleotide synthesis. The label is treated as if it were simply another base: It is inserted at the appropriate place in the sequence and incorporated automatically. • Specialty phosphoramidites allow online addition of the label during synthesis, saving time in production of labeled probes and primers • Designed and optimized for use with ABI™ synthesizers, guaranteeing reliable nucleic acid synthesis • Life Technologies offers a range of specialty phosphoramites and linkers...

Catalog: 403171
Size: 50 mg

TaqMan® Reverse Transcription Reagents (10-Pack)

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

This Applied Biosystems® kit provides the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, and oligo (dT) 16 , reverse primers are included for cDNA synthesis flexibility. Supplied as 2000 reactions in 10 tubes (200 reactions/tube). • Contains MultiScribe® Reverse Transcriptase, a recombinant Moloney Murine Leukemia Virus Reverse Transcriptase • Random hexamers and oligo (dT) 16 included for flexibility • Individual components provide versatility in assay set-up • Compatible with one-step or two-step RT-PCR reactions TaqMan® Reverse Transcription Reagents provide all...

Catalog: 4304134
Size: 2000 reactions

TaqMan® Reverse Transcription Reagents

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

This kit provides the indivdual components to perform reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and sequence-specific reverse primers provide flexibility for cDNA synthesis. • Contains MultiScribe® Reverse Transcriptase, a recombinant Moloney Murine Leukemia Virus Reverse Transcriptase • Random hexamers and oligo (dT)16 provide flexibility for cDNA synthesis • Individual components provide versatility in assay set-up • Compatible with one-step or two-step RT-PCR reactions Life Technologies has developed a comprehensive set of guidelines to ensure success when using Rea...

Catalog: N8080234
Size: 200 reactions

5-Ethynyl Uridine (EU)

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > BrdU, EdU & EU Reagents

Detect global RNA transcription temporally and spatially in both in-vitro and in-vivo with the modified nucleotide, ethynyl uridine (EU, E10345) or in kit form with Click-iT Nascent RNA capture kit, C-10365. Both approaches use powerful click chemistry detection, a simple and robust two-step technique. In step one, the alkyne-containing biomolecule is fed to cells or animals and actively incorporated into RNA. Detection utilizes the chemoselective ligation or “click" reaction between an azide and an alkyne where the modified protein is detected with a corresponding azide-containing dye or hapt...

Catalog: E-10345
Size: 5 mg

5-Bromo-2´-Deoxyuridine (BrdU)

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > BrdU, EdU & EU Reagents

Cells can naturally incorporate the thymidine analog BrdU into their cells during cell division, making this nucleoside analog an excellent marker of by cell cycle and proliferation. Analysis of incorporated BrdU can be with an anti-BrdU antibody.

Catalog: B23151
Size: 100 mg

EdU (5-ethynyl-2’-deoxyuridine)

Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > BrdU, EdU & EU Reagents

EdU (5-ethynyl-2’-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2’-deoxyuridine) and directly measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. Detection of EdU employs the copper(I) catalyzed click reaction with an azide modified fluorescent dye to form a stable triazole ring. Because of the small size of the click detection reagent, no harsh denaturation steps are needed to gain access to the DNA. Eliminating this step allows for more reproducible results, a s...

Catalog: E10415
Size: 5 g