Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Oligo(dT) Primers
These are the same Ambion® primers that are currently included in the RETROscript® Kit (SKU# AM1710). They are provided at a stock concentration of 50 µM and are functionally tested using the RETROscript® Kit.
Catalog: AM5730G
Size: 80 µl
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Oligo(dT) Primers
Applied Biosystems® Oligo (dT) 16 is a homo-oligomeric deoxyribonucleotide (poly dT) used for priming and reverse transcription of polyadenylated (poly A+) mRNA. Note : See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostic procedures.
Catalog: N8080128
Size: 1 kit
Category: Products & Services > Oligos, Primers, Probes & Nucleotides
5’-Fluorescent labeled oligo: 10,000 picomoles
Catalog: 450007
Size: 10,000 pmol
Category: Products & Services > Oligos, Primers, Probes & Nucleotides
5’ Phosphate labeled oligo
Catalog: 4326342
Size: 80,000 pmol
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > Fluorescently Labeled Nucleotides
Molecular Probes® ChromaTide® dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide® dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis. ChromaTide® Labeled Nucleotides Specifications: • Ex/Em of dye: Alexa Fluor® 594-5-dUTP (590/615 nm) • L...
Catalog: C-11400
Size: 25 µl
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > Fluorescently Labeled Nucleotides
BODIPY FL adenosine 5'-triphosphate (A-12410) and BODIPY FL guanosine 5'-triphosphate are composed of a BODIPY FL fluorophore attached to the 2' or 3' position of the ribose ring via a linker. Interactions between the fluorophore and the purine base are evident from the spectroscopic properties of these nucleotide analogs. The fluorescence quantum yield of BODIPY FL GTP is only about one-tenth that of BODIPY FL ATP; however, this yield may be enhanced upon binding of BODIPY FL GTP to GTP-binding proteins.
Catalog: G-12411
Size: 100 µl
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > Biotin Labeled Nucleotides
Biotin-14-dATP is a patented dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker. The long, 14-atom spacer arm allows accessibility to streptavidin for sensitive detection of probe-target hybrids in in situ and filter-based hybridizations. Biotin-14-dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP, dGTP, and dTTP (1). Biotin-14-dATP can be added to the 3´ end of DNA using terminal deoxynucleotidyl transferase (2). The amount supplied is sufficient for labeling up to 50 µg of DNA by nick translation. Performance and ...
Catalog: 19524-016
Size: 50 nmol
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > Fluorescently Labeled Nucleotides
Molecular Probes® ChromaTide® dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide® dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis. ChromaTide® Labeled Nucleotides Specifications: • Ex/Em of dye: Texas Red®-12-dUTP (595/615 nm) • Length...
Catalog: C-7631
Size: 25 µl
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > Fluorescently Labeled Nucleotides
The mixed-isomer analog of BODIPY® FL GDP is comprised of the BODIPY® FL fluorophore attached to the 2’ or 3’ position of the ribose ring via an aminoethylcarbamoyl linker. BODIPY® FL exhibits spectral characteristics similar to fluorescein (FITC) or the Alexa Fluor® 488 dye.
Catalog: G-22360
Size: 100 µl
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Random Primers
These are the same primers that are currently included in the RETROscript® Kit (SKU# AM1710). They are provided at a stock concentration of 50 µM and are functionally tested using the RETROscript® Kit.
Catalog: AM5722G
Size: 80 µl
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > Cap Analog & Variants
Ambion® Cap Analog [m 7 G(5')ppp(5')G] is used for the synthesis of 5' capped RNA molecules in in vitro transcription reactions. These molecules are translated more efficiently in in vitro translation systems than uncapped mRNAs. 25 A 254 U supplied. Substitution of cap analog for a portion of the GTP in an in vitro transcription reaction results in the incorporation of the cap structure into a corresponding fraction of the transcripts. Capped mRNAs are generally translated more efficiently in reticulocyte lysate and wheat germ in vitro translation systems. It is important that in vitro transc...
Catalog: AM8050
Size: 1 tube
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > RNAi Oligos > siRNA Libraries
For the first time, you can obtain the benefits of Ambion® Silencer ® Select siRNAs in a convenient pre-plated, library format. These siRNA collections enable medium and high throughput analysis of phenotypes resulting from inhibition of large sets of targets from specific functional classes. • Ready-to-ship collections of Ambion® Silencer ® Select siRNAs to popular human gene classes • Three individual siRNAs per target provided in a convenient 96-well format • Designed with an all new algorithm that yields better, more consistent knockdown • Novel chemical modifications reduce off-target eff...
Catalog: 4397918
Size: 1 set
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > dNTPs
This dNTP Set consists of all four deoxyribonucleoside triphosphates (dATP, dCTP, dGTP, dTTP), each at a concentration of 100 mM. They are suitable for use in PCR, sequencing, nick translation, fill-in, cDNA synthesis, and TdT tailing reactions, as well as for dilution of radiolabeled dNTPs.
Catalog: 10297-018
Size: 4 × 250 µL
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > BrdU, EdU & EU Reagents
Detect global RNA transcription temporally and spatially in both in-vitro and in-vivo with the modified nucleotide, ethynyl uridine (EU, E10345) or in kit form with Click-iT Nascent RNA capture kit, C-10365. Both approaches use powerful click chemistry detection, a simple and robust two-step technique. In step one, the alkyne-containing biomolecule is fed to cells or animals and actively incorporated into RNA. Detection utilizes the chemoselective ligation or “click" reaction between an azide and an alkyne where the modified protein is detected with a corresponding azide-containing dye or hapt...
Catalog: E-10345
Size: 5 mg
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > dNTPs
10 mM dNTP Mix, dATP, dCTP, dGTP, and dTTP (2´-deoxynucleoside 5´-triphosphates) are suitable for use in PCR, sequencing, nick transla-tion, fill-in, cDNA synthesis, and TdT tailing reactions, as well as for dilution of radiolabeled dNTPs.
Catalog: 18427-013
Size: 100 µl
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Random Primers
Applied Biosystems® Random Hexamers are short oligodeoxyribonucleotides of random sequence [d(N) 6 ] that anneal to random complementary sites on a target DNA or RNA, to serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase. With radiolabeled nucleotides present, the DNA is labeled quickly and reliably to high specific activity. Note : See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostics procedures.
Catalog: N8080127
Size: 1 kit
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > DNA Primers & Oligos > Sequencing Primers
The 5500 W REV1 (DNA) SP Kit contains sequencing primers and probes for DNA fragment libraries used in paired-end sequencing runs. The kit is used to prime the F5 tag for ligation sequencing on the 5500 W FlowChip V2 for DNA libraries. Each kit includes one strip tube containing sufficient specific sequencing primers and bridge probes to run 6 lanes.
Catalog: 4475684
Size: 1 kit
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > NTPs
ATP is an essential component for kinase assays using LanthaScreen™ Technology. Invitrogen's ATP is 10mM in water.
Catalog: PV3227
Size: 500 µl
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > BrdU, EdU & EU Reagents
Cells can naturally incorporate the thymidine analog BrdU into their cells during cell division, making this nucleoside analog an excellent marker of by cell cycle and proliferation. Analysis of incorporated BrdU can be with an anti-BrdU antibody.
Catalog: B23151
Size: 100 mg