Nupage Sample Reducing Agent Dtt Water
NuPAGE® Reducing Agent (10X) (10 ml) is used to reduce protein samples for protein gel electrophoresis. NuPAGE® Reducing Agent contains 500 mM dithiothreitol (DTT) at a ready-to-use 10X concentration in a stabilized liquid form. Use NuPAGE® Sample Reducing Agent when running proteins on NuPAGE® Bis-Tris gels to maintain protein samples in a reduced state during electrophoresis. For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
Catalog: NP0009
Size: 10 ml
Size: 10 ml
DTT is an essential component for kinase assays using LanthaScreen™ Technology. Invitrogen's DTT is 1M in water. It is tested for background fluorescence, making it optimal for use in high-throughput screening applications.
Catalog: P2325
Size: 1 ml
Size: 1 ml
Disufide crosslinks of cystines in proteins can be reduced to cysteine residues by dithiothreitol (DTT) .
Catalog: D-1532
Size: 1 g
Size: 1 g
NuPAGE® LDS Sample Buffer (4X) (10 ml) is used to prepare protein samples for denaturing gel electrophoresis with the NuPAGE® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing the protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate at a pH of 8.4, which allows for maximal activity of the reducing agent. For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X) . Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sample...
Catalog: NP0007
Size: 10 ml
Size: 10 ml
NuPAGE® LDS Sample Buffer (4X) (250 ml) is used to prepare protein samples for denaturing gel electrophoresis with the NuPAGE® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing the protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate at a pH of 8.4, which allows for maximal activity of the reducing agent. For native gel electrophoresis, use Novex® Tris-Glycine Native Sample Buffer (2X) . Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sampl...
Catalog: NP0008
Size: 250 ml
Size: 250 ml
Ambion® Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving. Because it is not DEPC treated, it is recommended for applications for which residual DEPC might prove detrimental (e.g. oocyte microinjection). This nuclease-free water is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
Catalog: AM9930
Size: 1 × 500 ml
Size: 1 × 500 ml
NuPAGE® Novex 3-8% Tris-Acetate Midi-Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. NuPAGE® Novex Tris-Acetate Midi-Gels (8 x 13 cm) can run up to 26 samples. The NuPAGE Midi-Gels run in the XCell4 Surelock™ Midi-Cell and, with a Midi Gel Adapter, can run in a Bio-Rad Criterion Cell. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity,...
Catalog: LA0050
Size: 1 kit
Size: 1 kit
Novex® Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both native and denatured proteins. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. Formulation: Novex® Tris-Glycine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recomme...
Catalog: LC2676
Size: 20 ml
Size: 20 ml
Ambion® RT-PCR grade water is tested for prokaryotic as well as eukaryotic genomic DNA contamination using an ultrasensitive PCR assay. It is provided in 10 x 1.5 mL. Prokaryotic DNA contamination is assessed by performing PCR with primers to 16S rRNA. Eukaryotic DNA contamination is assessed by performing PCR with primers to 18S rRNA. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity and is suitable for any PCR application.
Catalog: AM9935
Size: 10 × 1.5 ml
Size: 10 × 1.5 ml
NativePAGE™ 5% G-250 Sample Additive is added to samples prepared with the NativePAGE™ Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE™ gels. This Additive is also included in the NativePAGE™ Sample Prep Kit. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.
Catalog: BN2004
Size: 0.5 ml
Size: 0.5 ml
Gibco® Distilled Water is high quality water for use as a solvent in the preparation of cell culture media and laboratory reagents. Available in many sizes to meet your needs, Gibco® Distilled Water features: • Cell culture quality • Proven performance • Use in cell culture and molecular biology applications Cell culture quality Gibco® Distilled Water is prepared by distillation and filtration (0.1 µm), with no added substances. Life Technologies monitors the water quality on a routine basis. Conductivity, total organic carbon, endotoxin and bioburden are tested daily; metals are tested monthl...
Catalog: 15230-071
Size: 100 ml
Size: 100 ml
Highly purified water (at least ASTM Type III or ISO Grade 3) is required when using the M220 Focused-ultrasonicator™. One AFA-grade water bottle (800 ml) is sufficient for more than 50 uses.
Catalog: 4482280
Size: 1 each
Size: 1 each
UltraPure™ DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. It is 0.1-µm membrane-filtered and tested for DNase, RNase, and protease activity.
Catalog: 10977035
Size: 500 ml
Size: 500 ml
NuPAGE® Novex Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended Buffers: Run NuPAGE® Novex Tris-Acetate gels...
Catalog: EA03785BOX
Size: 1 box
Size: 1 box
Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Catalog: 11058-021
Size: 500 ml
Size: 500 ml
NuPAGE® Novex Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended Buffers: Run NuPAGE® Novex Tris-Acetate gels...
Catalog: EA0376BOX
Size: 1 box
Size: 1 box
NuPAGE® Antioxidant is a proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis. NuPAGE® Antioxidant is added to the running buffer in the upper (cathode) buffer chamber when performing protein gel electrophoresis under reducing conditions. NuPAGE® Antioxidant is provided in a 15 ml size. NuPAGE® Antioxidant must be used with samples reduced using NuPAGE® Sample Reducing Agent or other reducing agent. NuPAGE® Antioxidant is optimized for the neutral pH of NuPAGE® gels and is not compatible with other gel chemistries. NuPAGE® Antioxidant may also be ad...
Catalog: NP0005
Size: 15 ml
Size: 15 ml
Denaturing polyacrylamide TBE-Urea Gels resolve single-stranded DNA oligos or RNA into sharp, distinct bands. Novex® TBE-Urea Gels are optimized for the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies, and Northern blot analysis. Novex® TBE-Urea Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE-Urea Gels are made with high-purity reagents, including tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEME...
Catalog: LC6876
Size: 10 ml
Size: 10 ml
NuPAGE® Transfer Buffer (20X) (125 ml) is a buffer used to transfer proteins from NuPAGE® gels to membranes for western blotting. NuPAGE® Transfer Buffer can be used with the Novex® Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer. NuPAGE® Antioxidant may be used with NuPAGE® Transfer Buffer to enhance transfer of reduced proteins to membranes. For Research Use Only. Not intended for animal or human therapeutic or diagnostic use. Related Link • Learn more about other Invitrogen products for your protein transfer needs .
Catalog: NP0006
Size: 125 ml
Size: 125 ml
The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step. PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous ...
Catalog: 4318930
Size: 1 kit
Size: 1 kit
Nupage Sample Reducing Agent Dtt Water
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