Nucleic Acid Purification Kits & Reagents

RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Total Nucleic Acid Purification Kits

The Ambion® RecoverAll™ Total Nucleic Acid Isolation Kit is for the extraction of total nucleic acid from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. Sufficient reagents are included for 40 purifications from up to four 20 µm sections, or up to 35 mg of unsectioned core samples each. Features of the RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE: • Optimized for isolation of total nucleic acids, including microRNAs, from FFPE tissue • No overnight Proteinase K digestion required—deparaffinize in the morning and perform qRT-PCR in the afternoon • Typical yields are...
Catalog: AM1975
Size: 40 purifications

PureLink® PCR Purification Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > PCR Clean-Up Kits

PureLink® PCR Purification Kits provide rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR products (Figure 1). The simple PureLink® bind/wash/elute procedure: provides two Binding Buffer options for fragments >100 bp or >300 bp* can be performed in either single column or 96-well plate format takes Two proprietary buffers are supplied to meet the needs of different purification requirements. Use the Binding Buffer for routine purifications of double-stranded DNA fragments from 100 bp to 12 kb. Use Binding Buffer HC for removal of primer...
Catalog: K3100-01
Size: 50 reactions

LSI™ MagVet Express Isolation Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents

The automated MagVet system enables rapid, simple purification of both RNA and DNA with highly effective removal of PCR inhibitors. Universal Sample Preparation System for All Pathogens • Automated nucleic acid purification system utilizing magnetic separation technology • Choice of 2 magnetic particle processors adapted to the extraction flow: 15 and 96 samples • Easy and quick extraction validated for many pathogens and sample types used in animal health diagnostics • All validation data and manuals are available on request • Only one extraction kit for all pathogens (DNA and RNA) and all sa...
Catalog: 102055
Size: 480 tests

PrepMan® Ultra Sample Preparation Reagent

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents

The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step. PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous ...
Catalog: 4318930
Size: 1 kit

TRIzol® Reagent

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents

TRIzol® Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour. Key Features of TRIzol® Reagent: • Isolate RNA, DNA, and protein from the same sample • Superior lysis capability, even with difficult sample types • Optimized formulations and pro...
Catalog: 15596-026
Size: 100 ml

DNAzol® BD Reagent

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents

DNAzol® BD Reagent is a ready-to-use organic reagent specifically formulated for the isolation of genomic DNA from whole blood in about 30 minutes. • Specifically formulated for extraction of genomic DNA from whole blood • Optimized for the rapid Isolation of genomic DNA Efficient Isolation of Genomic DNA from Whole Blood DNAzol® BD Reagent is specifically formulated for the isolation of genomic DNA from whole blood. DNAzol® BD Reagent procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate. ...
Catalog: 10974-020
Size: 100 ml

LSI™ MagVet Express Isolation Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents

The automated MagVet system enables rapid, simple purification of both RNA and DNA with highly effective removal of PCR inhibitors. Universal Sample Preparation System for All Pathogens • Automated nucleic acid purification system utilizing magnetic separation technology • Choice of 2 magnetic particle processors adapted to the extraction flow: 15 and 96 samples • Easy and quick extraction validated for many pathogens and sample types used in animal health diagnostics • All validation data and manuals are available on request • Only one extraction kit for all pathogens (DNA and RNA) and all sa...
Catalog: 102051
Size: 96 tests

LSI™ MagVet Universal Isolation Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents

The automated MagVet system enables rapid, simple purification of both RNA and DNA with highly effective removal of PCR inhibitors. Universal Sample Preparation System for All Pathogens • Automated nucleic acid purification system utilizing magnetic separation technology • Choice of 2 magnetic particle processors adapted to the extraction flow: 15 and 96 samples • Easy and quick extraction validated for many pathogens and sample types used in animal health diagnostics • All validation data and manuals are available on request • Only one extraction kit for all pathogens (DNA and RNA) and all sa...
Catalog: MV384
Size: 384 tests

TRI Reagent®

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents

A complete, ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. • Simple—A single reagent for isolation of DNA-free RNA, RNA-free DNA, and protein • Productive—Obtain higher yields than with traditional guanidine thiocyanate or cesium chloride methods • Versatile—Works with many sample sources, including human, plant, yeast, bacterial, and viral samples • Flexible—Easily scalable depending on starting material and desired yield • Now incorporated into our RiboPure™ and MagMAX™-96 for Microarrays RNA Is...
Catalog: AM9738
Size: 100 ml

TRIzol® LS Reagent

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents

TRIzol® LS Reagent is a complete, ready-to-use reagent optimized for the isolation of high-quality total RNA, or the simultaneous isolation of RNA, DNA, and protein from a variety of liquid samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from liquid samples of human, animal, plant, yeast, bacterial, and viral origin, typically within one hour. Key Features of TRIzol® LS Reagent: • Formulated for use with liquid samples such as serum and virus preparations • Facilitates recovery of RNA, DNA, and protein...
Catalog: 10296-028
Size: 200 ml

PARIS™ Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid & Protein Isolation Kits

The Ambion® PARIS™ system is for the isolation of both RNA and native protein from the same experimental sample. The kit also permits separation of nuclear and cytoplasmic fractions prior to RNA and/or protein isolation. The kit contains sufficient reagents for 50 purifications from1 to 75 mg of tissue or 100 to 10 7 cells each. • Fast, simple procedure • Isolate nuclear and cytoplasmic RNA and protein from cultured cells • Isolate total RNA and protein from cultured cells or tissues • No phenol extraction or alcohol precipitation • Reduces time, cost, and variability between independent exper...
Catalog: AM1921
Size: 50 purifications

PureLink® HiPure Plasmid Maxiprep Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Plasmid DNA Purification Kits

The PureLink® HiPure Plasmid Purification Systems are designed to isolate transfection-grade plasmid DNA from E. coli in less than 2 hours. The Maxiprep Kit protocol will typically yield 500–850 µg plasmid DNA from 100–200 ml bacterial culture, at a purity that is comparable to that achieved with two passes through cesium chloride gradients. Advantages of the PureLink® HiPure Plasmid Maxiprep Kit include: • Labor- and time-saving —No need for extra steps to remove endotoxin and other contaminants • High purity, low endotoxin product —pure enough for mammalian cell transfection • Versatility —p...
Catalog: K2100-07
Size: 25 preps

PureLink® RNA Mini Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. • Rapid, Non-Hazardous RNA purification capabilities • Ready-to-use RNA for many downstream applications Rapid and Convenient Column Purification Procedures The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and t...
Catalog: 12183018A
Size: 50 preps

iPrep™ Trizol® Plus RNA Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

Use the iPrep™ Trizol® Plus RNA Kit for the highest purity, even from difficult samples such as fatty tissue. This kit provides reproducible RNA sample preparation from up to 50 mg of tissue, 10e7 cultured cells, 10 ml blood, and plant samples. The kit contains pre-filled, fully qualified cartridges to avoid any mix-ups or cross-contamination. It also provides low CVs and reduced purification variability, increasing the reliability of your gene expression assays.
Catalog: IS-10007
Size: 1 kit

MICROB Express ™ Bacterial mRNA Enrichment Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

This Ambion® kit is for the purification of bacterial mRNA by removing rRNA from total RNA. Sufficient reagents for 20 mRNA purifications, each from 10 µg of total RNA are provided. Features of the MICROB Express ™ Kit: • Dramatically increase the sensitivity of array analyses and other procedures • Removes >95% of 16S and 23S rRNA from bacterial RNA • Simple procedure takes less than 2 hours • Works with many Gram positive and Gram negative bacteria Rapid Purification of Bacterial mRNA is Now Possible Isolating mRNA from bacteria has been virtually impossible, until now. The MICROB Express ™ ...
Catalog: AM1905
Size: 20 reactions

RNAqueous® Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

The Ambion® RNAqueous® Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or 10 7 -10 8 cells using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The RNAqueous®-Midi Kit provides enough reagents for 15 RNA purifications. • Convenient syringe-based filtration • Compatible with vacuum manifolds when processing multiple samples The Ambion® RNAqueous® Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through th...
Catalog: AM1912
Size: 50 purifications