The Ambion® RecoverAll™ Total Nucleic Acid Isolation Kit is for the extraction of total nucleic acid from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. Sufficient reagents are included for 40 purifications from up to four 20 µm sections, or up to 35 mg of unsectioned core samples each. Features of the RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE: • Optimized for isolation of total nucleic acids, including microRNAs, from FFPE tissue • No overnight Proteinase K digestion required—deparaffinize in the morning and perform qRT-PCR in the afternoon • Typical yields are...
Size: 40 purifications
PureLink® PCR Purification Kits provide rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR products (Figure 1). The simple PureLink® bind/wash/elute procedure: provides two Binding Buffer options for fragments >100 bp or >300 bp* can be performed in either single column or 96-well plate format takes Two proprietary buffers are supplied to meet the needs of different purification requirements. Use the Binding Buffer for routine purifications of double-stranded DNA fragments from 100 bp to 12 kb. Use Binding Buffer HC for removal of primer...
Size: 50 reactions
This Ambion® kit is for the purification of bacterial mRNA by removing rRNA from total RNA. Sufficient reagents for 20 mRNA purifications, each from 10 µg of total RNA are provided. Features of the MICROB Express ™ Kit: • Dramatically increase the sensitivity of array analyses and other procedures • Removes >95% of 16S and 23S rRNA from bacterial RNA • Simple procedure takes less than 2 hours • Works with many Gram positive and Gram negative bacteria Rapid Purification of Bacterial mRNA is Now Possible Isolating mRNA from bacteria has been virtually impossible, until now. The MICROB Express ™ ...
Size: 20 reactions
A complete, ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. • Simple—A single reagent for isolation of DNA-free RNA, RNA-free DNA, and protein • Productive—Obtain higher yields than with traditional guanidine thiocyanate or cesium chloride methods • Versatile—Works with many sample sources, including human, plant, yeast, bacterial, and viral samples • Flexible—Easily scalable depending on starting material and desired yield • Now incorporated into our RiboPure™ and MagMAX™-96 for Microarrays RNA Is...
Size: 100 ml
The PureLink® HiPure Plasmid Purification Systems are designed to isolate transfection-grade plasmid DNA from E. coli in less than 2 hours. The Maxiprep Kit protocol will typically yield 500–850 µg plasmid DNA from 100–200 ml bacterial culture, at a purity that is comparable to that achieved with two passes through cesium chloride gradients. Advantages of the PureLink® HiPure Plasmid Maxiprep Kit include: • Labor- and time-saving —No need for extra steps to remove endotoxin and other contaminants • High purity, low endotoxin product —pure enough for mammalian cell transfection • Versatility —p...
Size: 25 preps
The ARCTURUS® Paradise® PLUS FFPE RNA Isolation Kit permits unprecedented gene expression analysis of millions of previously inaccessible samples by unlocking RNA from formalin-fixed, paraffin-embedded (FFPE) tissues. Amplify as little as 5 ng of the isolated fixed RNA using reagents and methods that have been specially optimized to overcome the challenges of cross-linked FFPE templates (Figure 1). Secure Downstream Success with the Paradise® QC Kit Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and th...
Size: 12 samples
The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step. PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous ...
Size: 1 kit
The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. • Rapid, Non-Hazardous RNA purification capabilities • Ready-to-use RNA for many downstream applications Rapid and Convenient Column Purification Procedures The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and t...
Size: 50 preps
Use the iPrep™ Trizol® Plus RNA Kit for the highest purity, even from difficult samples such as fatty tissue. This kit provides reproducible RNA sample preparation from up to 50 mg of tissue, 10e7 cultured cells, 10 ml blood, and plant samples. The kit contains pre-filled, fully qualified cartridges to avoid any mix-ups or cross-contamination. It also provides low CVs and reduced purification variability, increasing the reliability of your gene expression assays.
Size: 1 kit
The Ambion® RNAqueous® Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or 10 7 -10 8 cells using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The RNAqueous®-Midi Kit provides enough reagents for 15 RNA purifications. • Convenient syringe-based filtration • Compatible with vacuum manifolds when processing multiple samples The Ambion® RNAqueous® Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through th...
Size: 50 purifications
Successfully purify DNA fragments from 40 bp to 10 kb Proven purity made possible using traditional silica-based technology The PureLink® Gel Extraction Kit is designed to purify DNA fragments from agarose gels (1). The simple procedure uses a silica-based spin cartridge (Figure 1) to purify DNA fragments from 40 bp to 10 kb in in vitro transcription, restriction mapping, cloning, and labeling.
Size: 50 preps
TRIzol® Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour. Key Features of TRIzol® Reagent: • Isolate RNA, DNA, and protein from the same sample • Superior lysis capability, even with difficult sample types • Optimized formulations and pro...
Size: 100 ml
Fast, simple protocols - Purify your plasmid DNA in less than 2 hours Labor-saving - No need for extra steps to remove endotoxin and other contaminants Megapreps and Gigapreps save time by utilizing vacuum-assisted cartridges PureLink® HiPure Plasmid Purification Systems are designed to isolate plasmid DNA of the highest purity (1) with a wide scale range (Table 1). Simple protocols an anion-exchange resin allow you to purify plasmid DNA (Figure 1) at a quality equivalent to two passes through cesium chloride gradients - the most rigorous method for DNA purification. In less than 2 hours, DNA ...
Size: 4 preps
MagMAX™ DNA Multi-Sample 96-Well Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood and blood cards, tissue, mouse tails, buccal swabs, buffy coats, and cultured cells. These purification kits use MagMAX™ magnetic bead-based nucleic acid isolation technology to produce high yields of purified DNA, free from inhibitors that may affect downstream reactions such as genotyping assays, CE and next-generation sequencing, and high resolution melting analysis. The 96-prep and 5x 96-prep kits are designed for higher throughput and automat...
Size: 5 x 96 preps
The Ambion® MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. MEGAclear™ efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Each kit includes sufficient reagents for 20 reactions. • Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components • High recovery from 1 ng to 500 µg of input • Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections • Quick protocol—purify RNA in le...
Size: 20 rxns w⁄ Manual
The PureLink® Viral RNA/DNA Mini Kit is a nucleic acid purification system designed for fast and easy isolation of viral RNA or DNA from cell-free samples, such as serum, plasma, and cerebrospinal fluid. Starting sample volumes can be as high as 500 µl and elution volumes as low as 10 µl, allowing for a 50-fold concentration of nucleic acids. The higher target concentration obtained using the PureLink® Viral RNA/ DNA Mini Kit provides greater sensitivity and accuracy in downstream detection procedures such as real-time PCR or endpoint analysis. Specially formulated buffers enable the purificat...
Size: 50 preps
The Ambion® PARIS™ system is for the isolation of both RNA and native protein from the same experimental sample. The kit also permits separation of nuclear and cytoplasmic fractions prior to RNA and/or protein isolation. The kit contains sufficient reagents for 50 purifications from1 to 75 mg of tissue or 100 to 10 7 cells each. • Fast, simple procedure • Isolate nuclear and cytoplasmic RNA and protein from cultured cells • Isolate total RNA and protein from cultured cells or tissues • No phenol extraction or alcohol precipitation • Reduces time, cost, and variability between independent exper...
Size: 50 purifications