Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Total Nucleic Acid Purification Kits
The Ambion® RecoverAll™ Total Nucleic Acid Isolation Kit is for the extraction of total nucleic acid from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. Sufficient reagents are included for 40 purifications from up to four 20 µm sections, or up to 35 mg of unsectioned core samples each. Features of the RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE: • Optimized for isolation of total nucleic acids, including microRNAs, from FFPE tissue • No overnight Proteinase K digestion required—deparaffinize in the morning and perform qRT-PCR in the afternoon • Typical yields are...
Catalog: AM1975
Size: 40 purifications
Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
The cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.
Catalog: S11342
Size: 250 µl
Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population. • Impermeant to live cells • Excitation/Emission: 504/523 nm • Use with 488 Argon-ion laser • Works with mammalian cells and both Gram-positive and Gram-negative bacteria Simple Viability Determination SYTOX® Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, ...
Catalog: S7020
Size: 250 µl
Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
The cell-permeant SYTO 82 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.
Catalog: S11363
Size: 250 µl
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents
The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step. PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous ...
Catalog: 4318930
Size: 1 kit
Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Fluorescent Nucleic Acid Detection (FISH) Kits
The FISH Tag™ DNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ DNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. We also offer FISH Tag™ Kits in single colors. FISH Tag™ Kit Specificati...
Catalog: F32951
Size: 1 kit
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents
TRIzol® Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour. Key Features of TRIzol® Reagent: • Isolate RNA, DNA, and protein from the same sample • Superior lysis capability, even with difficult sample types • Optimized formulations and pro...
Catalog: 15596-026
Size: 100 ml
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents
DNAzol® BD Reagent is a ready-to-use organic reagent specifically formulated for the isolation of genomic DNA from whole blood in about 30 minutes. • Specifically formulated for extraction of genomic DNA from whole blood • Optimized for the rapid Isolation of genomic DNA Efficient Isolation of Genomic DNA from Whole Blood DNAzol® BD Reagent is specifically formulated for the isolation of genomic DNA from whole blood. DNAzol® BD Reagent procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate. ...
Catalog: 10974-020
Size: 100 ml
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents
The automated MagVet system enables rapid, simple purification of both RNA and DNA with highly effective removal of PCR inhibitors. Universal Sample Preparation System for All Pathogens • Automated nucleic acid purification system utilizing magnetic separation technology • Choice of 2 magnetic particle processors adapted to the extraction flow: 15 and 96 samples • Easy and quick extraction validated for many pathogens and sample types used in animal health diagnostics • All validation data and manuals are available on request • Only one extraction kit for all pathogens (DNA and RNA) and all sa...
Catalog: 102055
Size: 480 tests
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents
A complete, ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. • Simple—A single reagent for isolation of DNA-free RNA, RNA-free DNA, and protein • Productive—Obtain higher yields than with traditional guanidine thiocyanate or cesium chloride methods • Versatile—Works with many sample sources, including human, plant, yeast, bacterial, and viral samples • Flexible—Easily scalable depending on starting material and desired yield • Now incorporated into our RiboPure™ and MagMAX™-96 for Microarrays RNA Is...
Catalog: AM9738
Size: 100 ml
Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Fluorescent Nucleic Acid Detection (FISH) Kits
FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit , which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. FISH Tag™ Kit Specificatio...
Catalog: F32947
Size: 1 kit
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid & Protein Isolation Kits
The Ambion® PARIS™ system is for the isolation of both RNA and native protein from the same experimental sample. The kit also permits separation of nuclear and cytoplasmic fractions prior to RNA and/or protein isolation. The kit contains sufficient reagents for 50 purifications from1 to 75 mg of tissue or 100 to 10 7 cells each. • Fast, simple procedure • Isolate nuclear and cytoplasmic RNA and protein from cultured cells • Isolate total RNA and protein from cultured cells or tissues • No phenol extraction or alcohol precipitation • Reduces time, cost, and variability between independent exper...
Catalog: AM1921
Size: 50 purifications
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents
TRIzol® LS Reagent is a complete, ready-to-use reagent optimized for the isolation of high-quality total RNA, or the simultaneous isolation of RNA, DNA, and protein from a variety of liquid samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from liquid samples of human, animal, plant, yeast, bacterial, and viral origin, typically within one hour. Key Features of TRIzol® LS Reagent: • Formulated for use with liquid samples such as serum and virus preparations • Facilitates recovery of RNA, DNA, and protein...
Catalog: 10296-028
Size: 200 ml
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents
The automated MagVet system enables rapid, simple purification of both RNA and DNA with highly effective removal of PCR inhibitors. Universal Sample Preparation System for All Pathogens • Automated nucleic acid purification system utilizing magnetic separation technology • Choice of 2 magnetic particle processors adapted to the extraction flow: 15 and 96 samples • Easy and quick extraction validated for many pathogens and sample types used in animal health diagnostics • All validation data and manuals are available on request • Only one extraction kit for all pathogens (DNA and RNA) and all sa...
Catalog: 102051
Size: 96 tests
Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents
The automated MagVet system enables rapid, simple purification of both RNA and DNA with highly effective removal of PCR inhibitors. Universal Sample Preparation System for All Pathogens • Automated nucleic acid purification system utilizing magnetic separation technology • Choice of 2 magnetic particle processors adapted to the extraction flow: 15 and 96 samples • Easy and quick extraction validated for many pathogens and sample types used in animal health diagnostics • All validation data and manuals are available on request • Only one extraction kit for all pathogens (DNA and RNA) and all sa...
Catalog: MV384
Size: 384 tests
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA6.2/V5-DEST vector offers the following key features: •Cytomegalovirus (CMV) promoter for high-level expression • att R sites for Gateway® cloning, enabling recombination with att L-flanked fragments •C-terminal V5 tag for easy detection •Blasticidin resistance gene for efficient stable selection •Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli Gateway® Cloning To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® d...
Catalog: 12489-027
Size: 6 µg
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Yeast Expression Vectors
The PichiaPink™ Secretion Signal Set is a set of 8 DNA fragments encoding secretion signal sequences. The PichiaPink™ Secretion Signal Set is designed to be easily ligated in front of your gene. The PichiaPink™ Secretion Signal Set is designed for use with the pPINK-HC and pPINK-LC vectors. Optimize Secretion of Your Expressed Protein By adding one of the 8 secretion signal sequences to your gene, you can secrete your protein into the media to make it easier to harvest and purify. Pichia pastoris secretes few native proteins, so the bulk of the protein in the media will be yours. Each DNA sequ...
Catalog: A11155
Size: 8 each
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors
All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
Catalog: V921-20
Size: 20 µg
Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 12290-010
Size: 6 µg