Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Trypsin Inhibitors
Soybean Trypsin Inhibitor is one of several protease inhibitors found in soybeans. Trypsin is inhibited at a molar ratio of 1:1, while chymotrypsin and plasmin are inhibited to a lesser extent. Soybean trypsin inhibitor will also inhibit other serine proteases. Gibco® Soybean Trypsin Inhibitor, powder has an activity greater than 7,000 BAEE units per mg. A 0.5 mg/mL Soybean Trypsin Inhibitor stock solution should be made by dissolving the non-sterile powder in 0.01M Borate Buffer or Dulbecco's Phosphate Buffered Saline (DPBS), without calcium and magnesium. Filter sterilize the stock solution ...
Catalog: 17075-029
Size: 1 g
Category: Products & Services > Proteins, Enzymes, & Peptides > Enzyme Inhibitors
KT5720 is a potent, cell permeable inhibitor of PKA, and is a valuable tool in studies delineating cAMP-dependent signaling pathways, including activation of the transcription factor CREB. Inhibition of PKC, PKG, and MLCK is negligible. KT5720 is prepared by a chemical modification of K-252a. Format/Formulation: White solid
Catalog: PHZ1141
Size: 0.1 mg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
TCR ALPHA BETA, Hamster Anti-Mouse, (Alexa Fluor 488); Alexa Fluor 488 conjugated Monoclonal antibody specific to Mouse TCR α/β. This antibody is validated for use in Flow Cytometry. Anti-TCR α/β recognizes the expressed product of the TCRA/B gene.
Catalog: HM3620
Size: 1 ml
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
KAPPA-OPIOID RECEPTOR PAb; Unconjugated Polyclonal antibody specific to Mouse, Rat κ-Opioid Receptor, internal. This antibody is validated for use in Western Blot. Anti-κ-Opioid Receptor, internal recognizes the expressed product of the OPRK1 gene. Catalog number 44302G is same antibody as former catalog number 44302. 44302G contains 50% glycerol, which improves the stability and longevity of this antibody.
Catalog: 44-302G
Size: 10 blots
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
PDGFRALPHA [pY742] PAb; Unconjugated Polyclonal antibody specific to Mouse, (Human, Rat) PDGFRα [pY742]. This antibody is validated for use in Western Blot. Anti-PDGFRα [pY742] recognizes the expressed product of the Pdgfra gene.
Catalog: 44-1006
Size: 10 blots
Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits
The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of input total RNA in a single 20 µL reaction to single stranded cDNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Includes sufficient reagents for 1000 x 20 µL reactions. • Linear target amplification for real-time PCR • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost • 10-fold greater dynamic range than other kits cDNA from Total RNA The High Capacity cDNA Reverse Transcription Kit (for...
Catalog: 4374967
Size: 1000 reactions
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)
MEM ALPHA
Catalog: 12714010S
Size: 500 ml
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Lipid Kinases
The Class II PI3 kinases, including PIK3C2A, phosphorylate the 3 position of PI or PI4P. Though not as well studied as the Class I PI3 kinases, the Class II enyzmes have been linked to diverse, recpetor-mediated cellular processes including insulin signalling, neuronal survival, and growth factor signalling.
Catalog: PR9053A
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Lipid Kinases
The Class II PI3 kinases, including PIK3C2A, phosphorylate the 3 position of PI or PI4P. Though not as well studied as the Class I PI3 kinases, the Class II enyzmes have been linked to diverse, recpetor-mediated cellular processes including insulin signalling, neuronal survival, and growth factor signalling.
Catalog: PV5587
Size: 1 mg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Lipid Kinases
PIK3CD (p110 delta) is a class IA PI3K that elicits signaling cascasdes by phosphorylation of the 3 position of PIP2. Unlike the widely expressed Class IA alpha and beta enzymes, the delta isoform is expressed primarily in leukocytes. PIK3CD is coexpressed and copurified with the regulatory subunit PIK3R1.
Catalog: PR9026C
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Lipid Kinases
PIK3CD (p110 delta) is a class IA PI3K that elicits signaling cascasdes by phosphorylation of the 3 position of PIP2. Unlike the widely expressed Class IA alpha and beta enzymes, the delta isoform is expressed primarily in leukocytes. PIK3CD is coexpressed and copurified with the regulatory subunit PIK3R1.
Catalog: PV5274
Size: 1 mg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
The PKC family of serine⁄threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
Catalog: PR1455C
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
The PKC family of serine⁄threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
Catalog: PV3600
Size: 1 mg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
AKT1 (PKB alpha) is a serine⁄threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells.
Catalog: PR3878D
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
AKT1 (PKB alpha) is a serine⁄threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells.
Catalog: PV3599
Size: 1 mg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Lipid Kinases
PIK3CA (p110 alpha) is known to be involved in the PI 3-kinase⁄AKT signaling pathway, which plays an important role in regulating cell growth and apoptosis. PIK3CA is coexpressed and copurified with the regulatory subunit PI3KR1.
Catalog: PR8581C
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Lipid Kinases
PIK3CA (p110 alpha) is known to be involved in the PI 3-kinase⁄AKT signaling pathway, which plays an important role in regulating cell growth and apoptosis. PIK3CA is coexpressed and copurified with the regulatory subunit PI3KR1.
Catalog: PV4789
Size: 1 mg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
CAMK2A (CaMKII alpha) is a serine⁄threonine kinase that regulates a number of cellular functions including neuronal processes, gene expression, carbohydrate metabolism, cytoskeletal function, and calcium homeostatsis. Autophosphorylation of threonine 286 in the presence of Ca2+ and calmodulin activates CAMK2A with the ability to switch from calmodulin-dependent to a calmodulin independent activity.
Catalog: PR4586A
Size: 1 mg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
CAMK2A (CaMKII alpha) is a serine⁄threonine kinase that regulates a number of cellular functions including neuronal processes, gene expression, carbohydrate metabolism, cytoskeletal function, and calcium homeostatsis. Autophosphorylation of threonine 286 in the presence of Ca2+ and calmodulin activates CAMK2A with the ability to switch from calmodulin-dependent to a calmodulin independent activity.
Catalog: PR4586C
Size: 100 µg
Category: Products & Services > Kits & Assays > Biochemical Assay Kits > Nuclear Receptor Biochemical Assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4544: The LanthaScreen™ TR-FRET ER alpha Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential estrogen receptor (ER) alpha ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an ER alpha ligand-binding domain (ER alpha-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format. Agonist m...
Catalog: A15125
Size: 800 × 20 µl assays