Mrna Isolation

Dynabeads® CD34 Positive Isolation Kit

Category: Products & Services > Beads & Microspheres > Dynabeads® for Cell Isolation & Expansion > Dynabeads® for Human Cell Isolation

Use this kit to isolate a high purity and yield of human CD34+ progenitor stem cells. Positively isolated cells are bead and antibody-free, phenotypically unaltered and suitable for any downstream applications including flow cytometry, functional studies, and cell culture to produce dendritic cells (DC's). The Dynal® CD34 Progenitor Cell Isolation System can also be used to isolate CD34+ cells from rhesus monkey (Macaca mulatta). Positive isolation of human CD34+ progenitor stem cells with bead release Stem cells can be isolated directly from whole blood, cord blood or bone marrow Isolated CD3...
Catalog: 11301D
Size: 5 ml

Total Exosome Isolation (from cell culture media)

Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents

Total Exosome Isolation (from cell culture media) enables fast and efficient enrichment of intact exosomes from cell culture media samples. • Maximize recovery of intact exosomes for any type of downstream application • Easily isolate exosomes using our simple and reliable protocol • Avoid time-consuming ultra-centrifugation • Maximize flexibility—can be scaled up or down depending on the sample size Isolation of exosomes can be a tedious, non-specific, and difficult process. Total Exosome Isolation (from cell culture media) enables simple and reliable concentration of intact exosomes from cel...
Catalog: 4478359
Size: 50 ml

Total Exosome Isolation (from serum)

Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents

Total Exosome Isolation (from serum) enables fast and efficient enrichment of intact exosomes from blood serum samples. • Maximise recovery of intact exosomes for any type of downstream applications • Easily isolate exosomes with our simple and reliable protocol • Avoid time-consuming ultra-centrifugation • Maximize flexibility—can be scaled up or down depending on the sample size Isolation of exosomes can be a tedious, non-specific, and difficult process. Total Exosome Isolation (from serum) enables simple and reliable concentration of intact exosomes from blood serum samples with a protocol ...
Catalog: 4478360
Size: 6 ml

Exosome - Human CD63 Isolation/Detection (from cell culture media)

Category: Products & Services > Beads & Microspheres > Beads for Organelle Isolation

Exosome – Human CD63 Isolation/Detection enables the purification of CD63+ exosomes (also called extracellular vesicles and multi-vesicular bodies) from pre-enriched cell culture samples. These exosomes can then be detected using techniques such as flow cytometry, electron microscopy, or Western blotting. The exosomes must be pre-enriched before isolation. This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent. • Obtain highly pure CD63+ exosomes • "See" your sample during handling • Easily scalable protocol • Detec...
Catalog: 106-06D
Size: 3 ml

Exosome - Streptavidin for Isolation/Detection

Category: Products & Services > Beads & Microspheres > Beads for Organelle Isolation

Exosome - Streptavidin for Isolation/Detection, when combined with your choice of biotinylated primary antibody, enables the purification of exosomes (also called extracellular vesicles and multi-vesicular bodies) from pre-enriched samples. These exosomes can then be detected using techniques such as flow cytometry, electron microscopy, or Western blotting. The exosomes must be pre-enriched before isolation. This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent. • Maximum flexibility with your choice of biotinylate...
Catalog: 106-08D
Size: 3 ml

MICROB Express ™ Bacterial mRNA Enrichment Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

This Ambion® kit is for the purification of bacterial mRNA by removing rRNA from total RNA. Sufficient reagents for 20 mRNA purifications, each from 10 µg of total RNA are provided. Features of the MICROB Express ™ Kit: • Dramatically increase the sensitivity of array analyses and other procedures • Removes >95% of 16S and 23S rRNA from bacterial RNA • Simple procedure takes less than 2 hours • Works with many Gram positive and Gram negative bacteria Rapid Purification of Bacterial mRNA is Now Possible Isolating mRNA from bacteria has been virtually impossible, until now. The MICROB Express ™ ...
Catalog: AM1905
Size: 20 reactions

TaqMan® Array Human Transcription of mRNA, Fast 96-Well Plate

Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Transcription of mRNA 96-well Plate contains 44 assays to transcription of mRNA associated genes and 4 assays to candidate endogenous control genes. All assays are plated in duplicate.
Catalog: 4418839
Size: 1 Fast plate

Arcturus® Paradise® Plus RNA Extraction and Isolation

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > RNA Purification Kits

The ARCTURUS® Paradise® PLUS FFPE RNA Isolation Kit permits unprecedented gene expression analysis of millions of previously inaccessible samples by unlocking RNA from formalin-fixed, paraffin-embedded (FFPE) tissues. Amplify as little as 5 ng of the isolated fixed RNA using reagents and methods that have been specially optimized to overcome the challenges of cross-linked FFPE templates (Figure 1). Secure Downstream Success with the Paradise® QC Kit Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and th...
Catalog: KIT0312-I
Size: 12 samples

RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Total Nucleic Acid Purification Kits

The Ambion® RecoverAll™ Total Nucleic Acid Isolation Kit is for the extraction of total nucleic acid from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. Sufficient reagents are included for 40 purifications from up to four 20 µm sections, or up to 35 mg of unsectioned core samples each. Features of the RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE: • Optimized for isolation of total nucleic acids, including microRNAs, from FFPE tissue • No overnight Proteinase K digestion required—deparaffinize in the morning and perform qRT-PCR in the afternoon • Typical yields are...
Catalog: AM1975
Size: 40 purifications

Quantu mRNA ™ Universal 18S Internal Standard

Category: Products & Services > Controls, Standards & Ladders > Control Primers, Primer⁄Probe Sets & Templates

The Ambion® Quantu mRNA ™ 18S Internal Standard with Competimer™ technology uses specially modified Competimer™s of the same sequence as the normal 18S primers that cannot be extended. By adjusting the ratio of 18S Competimer™s to normal 18S mRNA primers, the signal for 18S mRNA can be attenuated to the level of very rare messages. They are used for quantitative end-point RT-PCR and include sufficient reagents for 100 reactions. Because of its invariant expression across tissues and treatments, 18S ribosomal RNA is an ideal internal control for quantitative RNA analysis. The Universal 18S Inte...
Catalog: AM1718
Size: 100 reactions

LSI™ MagVet Express Isolation Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents

The automated MagVet system enables rapid, simple purification of both RNA and DNA with highly effective removal of PCR inhibitors. Universal Sample Preparation System for All Pathogens • Automated nucleic acid purification system utilizing magnetic separation technology • Choice of 2 magnetic particle processors adapted to the extraction flow: 15 and 96 samples • Easy and quick extraction validated for many pathogens and sample types used in animal health diagnostics • All validation data and manuals are available on request • Only one extraction kit for all pathogens (DNA and RNA) and all sa...
Catalog: 102051
Size: 96 tests

LSI™ MagVet Express Isolation Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents

The automated MagVet system enables rapid, simple purification of both RNA and DNA with highly effective removal of PCR inhibitors. Universal Sample Preparation System for All Pathogens • Automated nucleic acid purification system utilizing magnetic separation technology • Choice of 2 magnetic particle processors adapted to the extraction flow: 15 and 96 samples • Easy and quick extraction validated for many pathogens and sample types used in animal health diagnostics • All validation data and manuals are available on request • Only one extraction kit for all pathogens (DNA and RNA) and all sa...
Catalog: 102055
Size: 480 tests

LSI™ MagVet Universal Isolation Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Nucleic Acid Purification Reagents

The automated MagVet system enables rapid, simple purification of both RNA and DNA with highly effective removal of PCR inhibitors. Universal Sample Preparation System for All Pathogens • Automated nucleic acid purification system utilizing magnetic separation technology • Choice of 2 magnetic particle processors adapted to the extraction flow: 15 and 96 samples • Easy and quick extraction validated for many pathogens and sample types used in animal health diagnostics • All validation data and manuals are available on request • Only one extraction kit for all pathogens (DNA and RNA) and all sa...
Catalog: MV384
Size: 384 tests

MagVet Mycobacterium paratuberculosis isolation kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Genomic DNA Purification Kits

The automated MagVet™ system enables rapid, simple purification of DNA with highly effective removal of PCR inhibitors. Features include: • Automated nucleic acid purification system utilizing magnetic separation technology • Choice of 2 magnetic particle processors adapted to the extraction flow: 15 and 96 samples • All validation data and manuals are available on request For veterinary use only. Regulatory requirements vary by country; products may not be available in your geographic area. WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of hu...
Catalog: MVMPT
Size: 192 tests

CellsDirect Resuspension & Lysis Buffers includes 10 ml Resuspension Buffer & 1 ml Lysis Buffer

Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents

These 2 buffers are to be used only as a complement to the SuperScript® III Platinum® CellsDirect Kits when large number of samples are processed. See manual of SuperScript® III Platinum® CellsDirect Kits for protocol recommendations.
Catalog: 11739-010
Size: 1 kit

PureLink® Air Porous Tape

Category: Products & Services > Instruments, Software, & Accessories > Tips, Tubes, Strips & Accessories > Isolation Accessories

The PureLink® Air Porous Tape is a component in the PureLink® 96 Plasmid Purification System. The PureLink® 96 Plasmid Purification offers a method for high throughput plasmid DNA isolation. The PureLink® Air Porous Tape allows for proper aeration of bacterial cultures.
Catalog: 12262-010
Size: 50 pieces