Mitf (Microphthalmia Transcription Factor)

TaqMan® Array Human Transcription of tRNA

Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays

The Applied Biosystems® TaqMan® Array Human Transcription of tRNA 96-well Plate contains 28 assays to transcription of tRNA associated genes and 4 assays to candidate endogenous control genes. All assays are plated in triplicate. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. • Affordable; m...
Catalog: 4414196
Size: 1 standard plate

High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of input total RNA in a single 20 µL reaction to single stranded cDNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Includes sufficient reagents for 1000 x 20 µL reactions. • Linear target amplification for real-time PCR • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost • 10-fold greater dynamic range than other kits cDNA from Total RNA The High Capacity cDNA Reverse Transcription Kit (for...
Catalog: 4374967
Size: 1000 reactions

TaqMan® Array Human Transcription of mRNA, Fast 96-Well Plate

Category: Products & Services > Slide & Chip Arrays > Real-Time PCR Arrays > Gene-Specific Arrays

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Transcription of mRNA 96-well Plate contains 44 assays to transcription of mRNA associated genes and 4 assays to candidate endogenous control genes. All assays are plated in duplicate.
Catalog: 4418839
Size: 1 Fast plate

TaqMan® Reverse Transcription Reagents (10-Pack)

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

This Applied Biosystems® kit provides the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, and oligo (dT) 16 , reverse primers are included for cDNA synthesis flexibility. Supplied as 2000 reactions in 10 tubes (200 reactions/tube). • Contains MultiScribe® Reverse Transcriptase, a recombinant Moloney Murine Leukemia Virus Reverse Transcriptase • Random hexamers and oligo (dT) 16 included for flexibility • Individual components provide versatility in assay set-up • Compatible with one-step or two-step RT-PCR reactions TaqMan® Reverse Transcription Reagents provide all...
Catalog: 4304134
Size: 2000 reactions

TaqMan® MicroRNA Reverse Transcription Kit, 200 Reactions

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

The Applied Biosystems® TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for 200 reactions of optimal performance in TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA. A highly specific kit that quantitates only mature miRNAs, not precursors. A sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is fast, simple and scalable 2-step quantitative RT-PCR assay that provides high-quality results in less than three hours.
Catalog: 4366596
Size: 200 reactions

TaqMan® Reverse Transcription Reagents

Category: Products & Services > Kits & Assays > cDNA Synthesis, Library, & Cloning Kits > cDNA Synthesis Kits

This kit provides the indivdual components to perform reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and sequence-specific reverse primers provide flexibility for cDNA synthesis. • Contains MultiScribe® Reverse Transcriptase, a recombinant Moloney Murine Leukemia Virus Reverse Transcriptase • Random hexamers and oligo (dT)16 provide flexibility for cDNA synthesis • Individual components provide versatility in assay set-up • Compatible with one-step or two-step RT-PCR reactions Life Technologies has developed a comprehensive set of guidelines to ensure success when using Rea...
Catalog: N8080234
Size: 200 reactions

mMESSAGE mMACHINE® T7 Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA; up to 39 µg of RNA per reaction • Extra convenience; cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped...
Catalog: AM1344
Size: 25 reactions

mMESSAGE mMACHINE® T3 Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA—up to 39 µg of RNA per reaction • Extra convenience—cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped R...
Catalog: AM1348
Size: 25 reactions

MEGAshortscript™ T7 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of large mass amounts of RNA produced from small templates ( • Efficiently transcribes small templates ( • Designed for use with plasmid, synthetic oligonucleotide, or PCR product templates Short transcripts require many more transcription initiation events to synthesize a given mass of product than do longer transcripts. Since the initiation step is the rate-limiting step in transcription reactions, the yield of product from short templates is often low. The MEGAshortscript™ High Yield Tra...
Catalog: AM1354M
Size: 25 rxns w⁄ Manual

MEGAscript® T7 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions. Each Ambion® kit includes sufficient reagents for 25 reactions. • Exclusive, ultrahigh yield technology is fast: 2 hour reaction time • Amplifies aRNA for gene array analysis and other applications • Efficiently incorporates many modified nucleotides A typical 20 µl MEGAscript® reaction with 1 µg of the pTRI-Xef-1alpha control template will yield over 100 µg of transcript. The versatility of the MEGAscript® k...
Catalog: AM1333M
Size: 25 rxns w⁄ Manual

FGF-Basic Recombinant Mouse Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines

Recombinant Mouse Fibroblast Growth Factor Basic (also known as FGF Basic, bFGF, FGF 2, FGF-β or Heparin Binding Growth Factor) is a bioactive protein intended for use in cell culture applications. FGF-basic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. • High purity —no interference from other proteins or contaminants • High biological activity —more results with less...
Catalog: PMG0031
Size: 100 µg

mMESSAGE mMACHINE® T7 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA; up to 39 µg of RNA per reaction • Extra convenience; cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped...
Catalog: AM1344M
Size: 25 rxns w⁄ Manual

5X mMESSAGE mMACHINE® T7 ULTRA Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

The Ambion® mMESSAGE mMACHINE™ T7 Ultra Kit combines a new cap analog—Anti-Reverse Cap Analog (ARCA)—with Ambion's patented high-yield transcription technology, to generate RNA transcripts that produce higher protein yields compared to other transcripts upon translation. The kit includes sufficient reagents for 50 reactions. • Synthesize more protein from in vitro –transcribed RNA • Express RNA transcripts more efficiently both in vitro and in vivo • Stabilize transcripts in vivo with included reagents for poly(A) tailing ARCA-capped RNA is Translationally More Active: A base modification in A...
Catalog: AMB1345-5
Size: 50 reactions

mMESSAGE mMACHINE® T3 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA—up to 39 µg of RNA per reaction • Extra convenience—cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped R...
Catalog: AM1348M
Size: 25 rxns w⁄ Manual

MEGAscript® T7 Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions. Each Ambion® kit includes sufficient reagents for 25 reactions. • Exclusive, ultrahigh yield technology is fast: 2 hour reaction time • Amplifies aRNA for gene array analysis and other applications • Efficiently incorporates many modified nucleotides A typical 20 µl MEGAscript® reaction with 1 µg of the pTRI-Xef-1alpha control template will yield over 100 µg of transcript. The versatility of the MEGAscript® k...
Catalog: AM1333
Size: 25 reactions

mMESSAGE mMACHINE® SP6 Kit with Manual

Category: Products & Services > Kits & Assays > Nucleic Acid Detection & Analysis Kits > Transcription Kits

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA • Extra convenience; cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped RNA Transcription will yield lar...
Catalog: AM1340M
Size: 25 rxns w⁄ Manual

EGFR Recombinant Human Protein (without Catalytic Activity Domain), hIgG1-Fc Tag

Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > Growth Factor⁄Cytokine⁄Chemokine Receptors

Epidermal growth factor receptor (EGFR), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 645) of human EGFR (NP_005219) fused to the Fc region of human IgG1 at the C-terminus...
Catalog: 10001-H02H-25
Size: 25 µg

EGFR Recombinant Human Protein (without Catalytic Activity Domain), hIgG1-Fc Tag

Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > Growth Factor⁄Cytokine⁄Chemokine Receptors

Epidermal growth factor receptor (EGFR), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 645) of human EGFR (NP_005219) fused to the Fc region of human IgG1 at the C-terminus...
Catalog: 10001-H02H-250
Size: 5 × 50 µg

EGFR Recombinant Human Protein (without Catalytic Activity Domain), hIgG1-Fc Tag

Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > Growth Factor⁄Cytokine⁄Chemokine Receptors

Epidermal growth factor receptor (EGFR), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 645) of human EGFR (NP_005219) fused to the Fc region of human IgG1 at the C-terminus...
Catalog: 10001-H02H-5
Size: 5 µg

EGFR Recombinant Human Protein (without Catalytic Activity Domain), hIgG1-Fc Tag

Category: Products & Services > Proteins, Enzymes, & Peptides > Receptors & Cell Surface Markers > Growth Factor⁄Cytokine⁄Chemokine Receptors

Epidermal growth factor receptor (EGFR), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies. This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 645) of human EGFR (NP_005219) fused to the Fc region of human IgG1 at the C-terminus...
Catalog: 10001-H02H-50
Size: 50 µg