Mirna Expression
The Ambion® pMIR-REPORT™ miRNA Expression Reporter Vector System is for the measurement of miRNA expression in cells. It contains two mammalian expression vectors-—one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency. Features of the pMIR-REPORT™ miRNA Expression Reporter Vector System: • Clone miRNA targets and evaluate miRNA regulation • Screen putative miRNA target sequences • Includes both reporter and control vectors The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell mea...
Catalog: AM5795M
Size: 1 kit w⁄ Manual
Size: 1 kit w⁄ Manual
The PichiaPink™ Expression Strain Set contains the 4 Pichia pastoris strains for use with the PichiaPink™ Expression System Kits. Each strain comes as a frozen stock in YPD media with 15% glycerol. All PichiaPink™ strains have the ade2 deletion. 3 strains have deletions of two protease genes, PEP4 and PRB1 . You can use the PichiaPink™ Expression Strain Set to refill your PichiaPink™ Yeast Expression System Kits. The PichiaPink™ Expression Strain Set includes the following Pichia strains: • PichiaPink™ Strain 1: ade2 • PichiaPink™ Strain 2: ade2 , pep4 • PichiaPink™ Strain 3: ade2 , prb1 • Pic...
Catalog: A11154
Size: 4 × 1ml
Size: 4 × 1ml
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Catalog: K6025-01
Size: 20 reactions
Size: 20 reactions
The pcDNA™3.1/V5-His TOPO® TA Expression Kit offers one-step cloning of Taq -amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.1/V5-His-TOPO® vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants. In addition, the vector includes the following features: Strong CMV promoter for high-level, constitutive expression. C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody. C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating...
Catalog: K4800-40
Size: 40 reactions
Size: 40 reactions
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Catalog: K101-01
Size: 20 reactions
Size: 20 reactions
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 12562-013
Size: 5 transfections
Size: 5 transfections
The Champion™ pET SUMO Expression System produces the highest levels of soluble protein in E. coli . It utilizes a small ubiquitin-related modifier (SUMO) fusion, belonging to the growing family of ubiquitin-related proteins, to enhance the solubility of expressed fusion proteins. In contrast to ubiquitin, SUMO is involved in the stabilization and localization of proteins in vivo . After expression, the 11 kd SUMO moiety can be cleaved by the highly specific and active SUMO (ULP-1) protease at the carboxyl terminal, producing a native protein*. The Champion™ pET SUMO Protein and Peptide Expres...
Catalog: K300-01
Size: 20 reactions
Size: 20 reactions
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Catalog: K4202-01
Size: 20 reactions
Size: 20 reactions
The FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein. Free your lab from the time-consuming, labor-intensive process of seeding, feeding, passing, and maintaining multiple flasks of adherent cells (Figure 1). The FreeStyle™ 293 Expression System combines GIBCO® FreeStyle™ 293 Expression Medium with 293fectin™, a cationic-lipid formulation designed to transfect suspension FreeStyle™ 293-F cells at high efficiency. FreeStyle™ 293-F cells and 293fectin™ are available as part of the Expression System or sep...
Catalog: K9000-01
Size: 1 kit
Size: 1 kit
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Catalog: K100-01
Size: 20 reactions
Size: 20 reactions
The pcDNA™3.1 Directional TOPO® Expression Kit uses linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO® for five-minute directional cloning and subsequent high-level expression. Directional Cloning technology facilitates expression experiments because: A proofreading enzyme is used for fewer errors in cloned genes Greater than 90% of the clones are in the correct orientation for expression, reducing time spent colony screening for clone orientation In addition, pcDNA3.1D/V5-His-TOPO® provides strong expression levels from the CMV promoter and the option of a C-terminal V5-His fusion t...
Catalog: K4900-40
Size: 40 reactions
Size: 40 reactions
The ViraPower™ HiPerform™ T-REx™ Gateway® Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral, T-REx™ and Gateway® technologies to facilitate easy recombination-based cloning and lentiviral-based, regulated (Tetracycline-inducible), high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄ TO⁄ V5-DEST vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscr...
Catalog: A11141
Size: 20 reactions
Size: 20 reactions
The mir Vana™ qRT-PCR Detection Kit has been optimized to amplify specific small RNA using qRT-PCR primer sets (not included), enabling accurate quantitation of miRNA expression levels. The novel detection system can be used to characterize miRNA expression via traditional endpoint RT-PCR or real-time RT-PCR using SYBR® Green I. • Quantitative results in less than 3 hours • Detection from as little as 25 pg of total RNA • Suitable for either real-time or end-point RT-PCR • Ideal for validating expression profiling data and high-throughput applications. MicroRNAs (miRNAs) are evolutionarily con...
Catalog: AM1558
Size: 200 reactions
Size: 200 reactions
The DES® TOPO® TA Expression Kit offers one-step cloning of PCR products directly into an inducible DES® expression vector. The kit uses the linearized, topoisomerase I-activated pMT/V5-His-TOPO® vector for fast and easy cloning and subsequent high-level expression. Topoisomerase activation of this vector allows PCR products to be ligated in just 5 minutes on your bench top to produce >85%recombinants. The pMT/V5-His-TOPO® vector has all of the features of the pMT/V5-His vector to simplify protein expression, detection, and purification of recombinant proteins in Drosophila S2 cells.
Catalog: K4125-01
Size: 20 reactions
Size: 20 reactions
The Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO® Expression Vectors (Figure 1) allow you to rapidly clone your gene and fuse it to the widely used and well characterized Fluorescent Proteins (FPs) from the jellyfish Aequorea victoria (1, 2). These powerful TOPO® cloning vectors contain the Emerald Green Fluorescent Protein (EmGFP) or the Yellow Fluorescent Protein (YFP) for simple, non-invasive detection of recombinant protein (Figure 2). Both FPs have been humanized for optimal mammalian expression (3). The Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO® Expression Vectors offer: • Top...
Catalog: K359-20
Size: 20 reactions
Size: 20 reactions
The Baculovirus Expression System with Gateway® Technology is designed to create recombinant pFastBac™ plasmids containing the polyhedrin promoter. The Baculovirus Expression System with Gateway® Technology offers: Expression using the Bac-to-Bac® technology (1) Destination vectors carrying the polyhedrin promoter for production of native (pDEST™8), N-terminal histidine fusion (pDEST™10), and N-terminal GST fusion (pDEST™20) proteins pDEST™10 vector containing a TEV protease cleavage site for removal of the fusion tag after protein purification (2)
Catalog: 11827-011
Size: 20 reactions
Size: 20 reactions
The ViraPower™ HiPerform™ Lentiviral FastTiter™ TOPO® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral and TOPO® technologies to facilitate quick PCR-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti7.3/V5-TOPO® vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central ...
Catalog: K5320-00
Size: 1 kit
Size: 1 kit
The ViraPower™ Lentiviral Directional TOPO® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. It combines Invitrogen’s ViraPower™ Lentiviral and Directional TOPO® technologies to facilitate quick PCR-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6⁄V5-D-TOPO® vector has the CMV promoter for driving constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells. Advantages • Lentivirus...
Catalog: K4950-00
Size: 1 kit
Size: 1 kit
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/GW/D-TOPO vector offers the following key features: •Cytomegalovirus (CMV) promoter for high-level expression •Adapted for Directional TOPO® Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation •Neomycin resistance gene for stable selection •C-terminal V5 tag for easy detection •Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli TOPO® Cloning Using restriction enzymes to clone your ge...
Catalog: K2440-20
Size: 20 reactions
Size: 20 reactions
This Ambion® kit is for the purification of bacterial mRNA by removing rRNA from total RNA. Sufficient reagents for 20 mRNA purifications, each from 10 µg of total RNA are provided. Features of the MICROB Express ™ Kit: • Dramatically increase the sensitivity of array analyses and other procedures • Removes >95% of 16S and 23S rRNA from bacterial RNA • Simple procedure takes less than 2 hours • Works with many Gram positive and Gram negative bacteria Rapid Purification of Bacterial mRNA is Now Possible Isolating mRNA from bacteria has been virtually impossible, until now. The MICROB Express ™ ...
Catalog: AM1905
Size: 20 reactions
Size: 20 reactions
Mirna Expression
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