Category: Products & Services > Kits & Assays > Impurity & Contamination Detection Kits > Mycoplasma Detection Kits
The MycoFluor™ Mycoplasma Detection Kit provides an ultrasensitive, rapid and simple fluorescence microscopic acid for the visual identification of mycoplasma infection in laboratory cell cultures. In order to detect mycoplasma, the fluorescent MycoFluor™ reagent is added directly to the culture medium, with or without cells, and the stained sample is then examined under a fluorescence microscope.
Catalog: M7006
Size: 1 kit
Category: Products & Services > Kits & Assays > Cell Analysis Kits > IHC & ICC Kits
The SuperPicture™ Polymer Detection Kit features our proprietary and enhanced HRP polymer technology to provide excellent sensitivity, high specificity, and a shorter protocol than conventional immunohistochemical staining methods. Features of the SuperPicture™ Polymer Detection Kit: • Reactivity: Mouse, rabbit, guinea pig, and rat • Applications: Immunohistochemistry (FFPE) Our new polymer conjugation methods have resulted in a versatile polymer capable of intense nuclear, cytoplasmic, and membrane antigen staining. The polymer conjugate contains multiple HRP molecules to provide excellent se...
Catalog: 87-9663
Size: 1 kit
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Autophagy Detection Kits
The Premo™ Autophagy Sensor RFP-p62 Kit combines the ability to monitor the induction or inhibition of autophagy, through the localization of the autophagy receptor p62 (also known as SQSTM1), with the high transduction efficiency and minimal toxicity of BacMam 2.0 technology. To perform an image-based analysis for autophagy, simply add the BacMam 2.0 RFP-p62 reagent to your mammalian cells, incubate overnight to ensure adequate protein expression, and then visualize using standard RFP (red fluorescent protein) settings. The RFP gene included in this chimera is mKate2. This monomeric fluoresce...
Catalog: P36241
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Protein Labeling Kits
The Click-iT® HPG Alexa Fluor® 488 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor® 488 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 488 azide leads to a chemoselective ligation or "click" reaction between the...
Catalog: C10428
Size: 1 kit
Category: Products & Services > Kits & Assays > Cell Analysis Kits > IHC & ICC Kits
The HistoMouse-Plus kit is the 2nd generation HistoMouse kit for the detection of mouse primary antibodies on mouse tissue samples without causing background due to endogenous mouse Ig. Our patented BEAT™ technology (Blocking Endogenous Antibody Technology) is used to prevent the secondary antibody conjugate from reacting with endogenous mouse Ig. The LAB-SA detection method is used to amplify the signal. In addition, our HistoMouse-Plus kit includes the AEC Single Solution chromogen⁄ substrate system to create an intense red deposit around the antigen⁄antibody⁄enzyme complex in the sample. Re...
Catalog: 85-9541
Size: For 50 Slides
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Autophagy Detection Kits
The Premo™ Autophagy Sensor GFP-p62 Kit combines the ability to monitor the induction or inhibition of autophagy, through the localization of the autophagy receptor p62 (also known as SQSTM1), with the high transduction efficiency and minimal toxicity of BacMam 2.0 technology. To perform an image-based analysis for autophagy, simply add the BacMam 2.0 GFP-p62 reagent to your mammalian cells, incubate overnight to ensure adequate protein expression, and then visualize using standard GFP (green fluorescent protein) settings. The GFP gene included in this chimera is TagGFP2, which has been demons...
Catalog: P36240
Size: 1 kit
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Autophagy Detection Kits
The Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B Kit combines the ability to monitor the various stages of autophagy (through LC3B protein localization) with the high transduction efficiency and minimal toxicity of BacMam 2.0 expression technology. To perform image-based analysis for autophagy, simply add the BacMam 2.0 RFP-GFP-LC3B reagent to your mammalian cells, incubate overnight to ensure maximum protein expression, and then visualize using standard GFP (green fluorescent protein) and RFP (red fluorescent protein) settings. This tandem RFP-GFP sensor capitalizes on the pH difference betwee...
Catalog: P36239
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Antibody & Protein Labeling Kits > Protein Labeling Kits
The Click-iT® HPG Alexa Fluor® 594 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor® 594 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 594 azide leads to a chemoselective ligation or “click" reaction between the...
Catalog: C10429
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains
The NeuroTrace™ BDA-10,000 Neuronal Tracer Kit contains the neuronal tracer, biotin dextran amine (BDA), and the detection reagents, avidin-horseradish peroxidase (avidin-HRP) and diaminobenzedine (DAB) for subsequent visualization by light or electron microscopy.
Catalog: N-7167
Size: 1 kit
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores
The Calcium Calibration Buffer Kits are designed to help determine the dissociation constant (K d ) of fluorescent Ca 2+ indicators using a fluorometer or quantitative imaging system. The Kd of an indicator can be calculated from a plot generated by scanning the excitation or emission of the indicator in the presence of eleven different Ca 2+ concentrations. Calcium Calibration Kit #1 contains 50 mL of 10 mM K 2 EGTA and 50 mL of 10 mM CaEGTA. Both solutions contain 100 mM KCl and 30 mM MOPS, pH 7.2 and are prepared in deionized water. These stock solutions can be blended to make buffers havin...
Catalog: C-3008MP
Size: 1 kit
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Apoptosis Kits
CellEvent® Caspase-3/7 Green ReadyProbes™ Reagent is a fluorogenic, no-wash indicator of activated caspase-3/7 for live- and fixed-cell applications. Activation of caspase-3 is an early indicator of apoptosis and CellEvent® Caspase-3/7 Green reagent allows rapid and sensitive detection of cells destined for cell death. Like other ReadyProbes™ reagents, CellEvent™ Caspase-3/7 Green reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells. CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent provides...
Catalog: R37111
Size: 1 kit
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Cell Metabolic Assay Kits
2-NBDG is a fluorescent glucose analog that has been used to monitor glucose uptake in live cells, as an indicator of cell viability. Although sensitive to its environment NBD fluorescence typically displays excitation/emission maxima of ~465/540 nm and can be visualized using optical filters designed for fluorescein.
Catalog: N13195
Size: 5 mg
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Phagocytosis Kits & Phagocytic Particles
The Molecular Probes® BioParticles® product line consists of a series of fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities. These fluorescent BioParticles® products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry. We offer E. coli (K-12 strain), S. aureus (Wood strain, without protein A) and zymosan ( S. cerevisiae ) BioParticles® conjugates covalently labeled with a variety of different fluorophores (special care has been taken to remove free dy...
Catalog: E-2864
Size: 10 mg