Microplate Reader
The RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensur...
Catalog: R33700
Size: 1 kit
Size: 1 kit
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+ . Fluo-3 has been used to image the spatial dynamics of Ca 2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt ...
Catalog: F-14200
Size: 500 µg
Size: 500 µg
The Mouse IL-6 ELISA research use only kit is designed to detect and quantify the level of mouse IL-6 in serum, plasma, and supernatant using 96-well plates and a microplate reader. Easy to run Rapid protocol Convenient precoated, removable 8-well strips Easy-to-Run Sandwich ELISA The total assay incubation time is only 3.5 hours. A monoclonal capture antibody specific for Mouse IL-6 has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known Mouse IL-6 content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinyla...
Catalog: KMC0062
Size: 192 tests
Size: 192 tests
Our Quant-iT™ OliGreen® ssDNA reagent is an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. This reagent is also available in a kit complete with buffer and oligonucleotide standard ([PRODUCT O11492]).
Catalog: O7582
Size: 1 ml
Size: 1 ml
Using the PicoGreen® dsDNA quantitation assay, you can selectively detect as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides (1). The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products. PicoGreen® dsDNA Quantitation Reagents are: Orders of magnitude more sensitive than UV absorbance readings, saving on precious sample (Figure 1) Specific for dsDNA in the presence of equimolar amounts of RNA (Figur...
Catalog: P7581
Size: 1 ml
Size: 1 ml
The Vybrant® Cell Adhesion Assay Kit is a fast and sensitive assay for measuring cell-cell or cell-surface adhesion for a variety of cell types. In this assay, cells are labeled with calcein AM and allowed to adhere. After removal of nonadherent cells, calcein fluorescence is used to calculate the number of adherent cells.
Catalog: V-13181
Size: 1 kit
Size: 1 kit
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4381: The LanthaScreen™ TR-FRET Androgen Receptor (AR) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential AR ligands as agonists of coactivator recruitment or antagonists of agonist-dependent coactivator recruitment. In both the agonist and antagonist modes, the homogeneous mix-and-read assay uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a rat AR ligand-binding domain (AR-LBD) that is tagged with hexahisti...
Catalog: A15119
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4544: The LanthaScreen™ TR-FRET ER alpha Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential estrogen receptor (ER) alpha ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an ER alpha ligand-binding domain (ER alpha-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format. Agonist m...
Catalog: A15125
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4541: The LanthaScreen™ TR-FRET Estrogen Receptor beta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ER beta ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a ER beta ligand-binding domain (ER beta-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format. Agonist mode To run...
Catalog: A15124
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4663: The LanthaScreen™ ERR (estrogen related receptor) alpha TR-FRET Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential ERR alpha ligands as inverse agonists (sometimes referred to as antagonists) of coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an ERR alpha ligand-binding domain (ERR alpha-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-rea...
Catalog: A15130
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4408: The LanthaScreen™ TR-FRET Estrogen Related Receptor (ERR) gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ERR gamma ligands as antagonists/inverse agonists of coactivator displacement. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (Figure 1). How it works The LanthaScreen™ TR...
Catalog: A15121
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4683: The LanthaScreen™ TR-FRET GR (glucocorticoid receptor) Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential GR ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant GR ligand-binding domain (GR-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format. Agonist mode: ...
Catalog: A15132
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4655: The LanthaScreen™ TR-FRET LXR (liver X receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR alpha ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR alpha ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format. In the LanthaScreen™ TR-FRET LXR alpha Coactivator ...
Catalog: A15128
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4684: The LanthaScreen™ TR-FRET PPAR (peroxisome proliferator activated receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential PPAR alpha ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant PPAR alpha ligand-binding domain (PPAR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a ho...
Catalog: A15133
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4685: The LanthaScreen™ TR-FRET PPAR (peroxisome proliferator activated receptor) delta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential PPAR delta ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant PPAR delta ligand-binding domain (PPAR delta-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous ...
Catalog: A15134
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4548: The LanthaScreen™ TR-FRET PPAR gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential PPAR gamma ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a Peroxisome Proliferator Activated Receptor (PPAR) gamma ligand-binding domain (PPAR gamma-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-...
Catalog: A15126
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4666: The LanthaScreen™ TR-FRET PR (Progesterone Receptor) Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential PR ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a PR ligand--binding domain (PR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format. When using the LanthaScreen™ TR-F...
Catalog: A15131
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4409: The LanthaScreen™ TR-FRET Retinoic Acid Receptor (RAR) alpha Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential RAR alpha ligands as agonists of coactivator recruitment. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1). How it works The LanthaScreen™ TR-FRET RAR alpha Coactivat...
Catalog: A15122
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
Microplate Reader
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