The RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensur...
Size: 1 kit
The Mouse IL-6 ELISA research use only kit is designed to detect and quantify the level of mouse IL-6 in serum, plasma, and supernatant using 96-well plates and a microplate reader. Easy to run Rapid protocol Convenient precoated, removable 8-well strips Easy-to-Run Sandwich ELISA The total assay incubation time is only 3.5 hours. A monoclonal capture antibody specific for Mouse IL-6 has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known Mouse IL-6 content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinyla...
Size: 192 tests
This Antibody Pair Kit comes with matched antibody pairs to detect and quantify protein levels of rat IL-6. Also available is Buffer Kit for Antibody Pairs that saves time with premade, easy-to-use buffers and solutions optimized for use with Invitrogen Antibody Pairs. The Assay Buffer can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Buffer reagents needed to complete the reaction are sold separately under Catalog No. CNB0011.
Size: 10 plates
Our Quant-iT™ OliGreen® ssDNA reagent is an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. This reagent is also available in a kit complete with buffer and oligonucleotide standard ([PRODUCT O11492]).
Size: 1 ml
alamarBlue® is a redox indicator that yields a colorimetric change and a fluorescent signal in a response to a metabolic activity. It is a proven safe and non-toxic dye used for quantitative analysis of cell viability and cell proliferation, for cytokine bioassays and in vitro cytotoxicity studies. Applications: • Cell proliferation determinations —alamarBlue® is water soluble, stable in culture and non-toxic • Cell viability assays, including cytotoxicity —metabolic activity and dye generation changes in proportion to altered viability and cell damage • Cytokine assays —measure cytokine-induc...
Size: 100 ml
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+ . Fluo-3 has been used to image the spatial dynamics of Ca 2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt ...
Size: 500 µg
Using the PicoGreen® dsDNA quantitation assay, you can selectively detect as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides (1). The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products. PicoGreen® dsDNA Quantitation Reagents are: Orders of magnitude more sensitive than UV absorbance readings, saving on precious sample (Figure 1) Specific for dsDNA in the presence of equimolar amounts of RNA (Figur...
Size: 1 ml
Our highly purified lucigenin is a fluorescent Cl- indicator that has excitation/emission maxima of 368/505 nm and whose fluorescence is quenched in the presence of Cl-. The dye is useful for determining Cl- concentrations in liposomes, reconstituted membranes or in extracellular medium but is not useful for intracellular measurements.
Size: 10 mg
The Vybrant® Cell Adhesion Assay Kit is a fast and sensitive assay for measuring cell-cell or cell-surface adhesion for a variety of cell types. In this assay, cells are labeled with calcein AM and allowed to adhere. After removal of nonadherent cells, calcein fluorescence is used to calculate the number of adherent cells.
Size: 1 kit
The IL-1RA ELISA Kit is designed to quantify protein levels of human interleukin-1 receptor antagonist (IL-1RA) in biological samples. IL-1RA is a cytokine which opposes the actions of IL-α and IL-1β by binding to IL-1 receptors.
Size: 96 tests
The IFN-β Mouse ELISA Kit quantifies natural and recombinant mouse IFN-β in serum and cell culture supernatant. Interferon-β (IFN-β) is a cytokine that is produced by the cells of the immune system.
Size: 96 tests
The RediPlate™ 96 RiboGreen® RNA Quantitation Kit is a rapid, simple, and highly sensitive fluorescence-based RNA-quantitation assay. The microplate has been pre-loaded with RiboGreen® Reagent, which shows bright green fluorescence upon binding to RNA or DNA (1). You simply add buffer and samples to the microplate wells, wait 10 minutes, and then read the fluorescence in any standard fluorescence-based microplate reader. The versatile RediPlate™ 96 design is ideal for either high-throughput quantitation or the measurement of a small number of samples. The RediPlate™ 96 RiboGreen® RNA Quantitat...
Size: 1 kit
The Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit contains a sensitive, one-step assay that uses the Amplex® Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) to detect hydrogen peroxide (H 2 O 2 ) or peroxidase activity. The Amplex® Red reagent, in combination with horseradish peroxidase (HRP), has been used to detect H 2 O 2 released from biological samples, including cells, or generated in enzyme-coupled reactions. Furthermore, Amplex® Red reagent can be used as an ultrasensitive assay for peroxidase activity when H 2 O 2 is in excess. In the presence of peroxidase, the Amplex® Red reag...
Size: 1 kit
To permit simultaneous long-term tracing of mixed-cell populations using different fluorescent colors, we have developed a probe whose applications should be similar to those of CFDA SE (commonly called CFSE). Cells labeled with our SNARF-1 carboxylic acid, acetate, succinimidyl ester have orange-red fluorescence that can be easily distinguished from that of cells loaded with green-fluorescent tracers such as CFDA SE.
Size: 10 × 50 µg
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4381: The LanthaScreen™ TR-FRET Androgen Receptor (AR) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential AR ligands as agonists of coactivator recruitment or antagonists of agonist-dependent coactivator recruitment. In both the agonist and antagonist modes, the homogeneous mix-and-read assay uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a rat AR ligand-binding domain (AR-LBD) that is tagged with hexahisti...
Size: 800 × 20 µl assays