mhc class ii i-ab

HLADR (class II) Mouse Anti-Human mAb (clone Tü36), Pacific Green™ Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

The HLA-DR, clone Tü36, Pacific Green™ conjugate is a monoclonal mouse antibody that recognizes the class II human leukocyte (HLA)-DR antigens. The Pacific Green™ dye is a Molecular Probes® fluorophore that has an absorbance at 411 nm and is optimally excited by the violet laser. Pacific Blue™, Pacific Green™, and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm, and 551 nm, respectively, facilitating three-color analysis. Antibody Specifications Description: HLA-DR, Mouse IgG2b monoclonal Label: Pacific Green™ dye Validated Application: Flow Cy...
Catalog: H11212
Size: 125 µl

PKA II beta [pS114] (Regulatory Domain) Rabbit Polyclonal Antibody

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

PKA REG 11BETA [pS114) PAb; Unconjugated Polyclonal antibody specific to Mouse, (Human, Rat) PKA IIβ [pS114] (regulatory domain). This antibody is validated for use in Western Blot. Anti-PKA IIβ [pS114] (regulatory domain) recognizes the expressed product of the PRKAR2B gene. Catalog number 44998G is same antibody as former catalog number 44998. 44998G contains 50% glycerol, which improves the stability and longevity of this antibody.
Catalog: 44-998G
Size: 10 blots

Gel Loading Buffer II (Denaturing PAGE)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Loading Buffers

A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue. Supplied in one 1.4 mL tube. All Ambion® Gel Loading Solutions are rigorously tested for non-specific endonuclease activity, exonuclease activity, RNase activity, and for functionality. These are the same solutions we use in-house and in our kits.
Catalog: AM8546G
Size: 1 ml

Luminescence Enhancer For Membrane Blotting Assays, Nitro-Block-II™ Enhancer

Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Chemiluminescent Substrates

This concentrated Nitro-Block-II™ Enhancer can be used with CSPD® or CDP-Star® chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II™ membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF membranes. • Chemically optimized formulations provide enhanced signal intensity in blotting applications with dioxetane-based substrate solutions (CSPD® or CDP-Star® substrates) on nitrocellulose or PVDF membranes. • Enhancer increases signal intensity, allowing decreased signal acquisition time. Increa...
Catalog: T2184
Size: 20 ml

Isotype Control For Rabbit Primary Ab

Category: Products & Services > Antibodies & Secondary Detection Reagents > Isotype Antibody Controls

Properly controlled IHC stains require that a non-immune immunoglobulin equivalent of the primary antibody be run on the sample tissue in the same way that the primary antibody is run. Any positive staining would indicate that the primary antibody stain may not be specific. These reagents can be used as negative isotype controls for our predilute primary antibodies.
Catalog: 08-6199
Size: 18 ml

XCell II™ Blot Module CE Mark

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Blotting Systems

The XCell II™ Blot Module allows you to easily transfer proteins or nucleic acids from mini-gels to membranes. It fits neatly into the XCell SureLock ™ and XCell II™ Mini-Cells in place of the gel/buffer core assembly. It requires less than 200 ml of transfer buffer for western, Southern, and northern transfers. Tough platinized titanium and stainless steel electrodes create a uniform electrical field without clamps or hinged gel holders. Maximum blot size is 9 cm x 9 cm.
Catalog: EI9051
Size: 1 unit

Collagenase, Type II, powder

Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Collagenase

Collagenase is a protease with specificity for the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Glyc-Pro. This sequence is found in high frequency in collagen. Collagenase is unique among proteases in its ability to degrade the triple-helical native collagen fibrils commonly found in connective tissues such as skin, tendon, blood vessels, and bone. Collagenase disaggregation has been suitable for the culture of human tumors, mouse kidney, human adult and fetal brain, and many other tissues including epithelium. It is relatively gentle, dissociates well at physiologic...
Catalog: 17101-015
Size: 1 g

Zero Blunt® TOPO® PCR Cloning Kit (with pCR®-Blunt II-TOPO®) with One Shot® TOP10 Chemically Competent E. coli and PureLink™ Quick Plasmid Miniprep Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > PCR Cloning Vectors

Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR®-Blunt II-TOPO® Vector for 5-minute benchtop ligations without ligase (1). The pCR®-Blunt II-TOPO® Vector also features: • ccdB gene for positive selection (2) • Eco R I sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli • SP6 promoter/primer site for...
Catalog: K2800-02
Size: 20 preps

AB-Human Serum

Category: Products & Services > Controls, Standards & Ladders > Control Serum, Cell Extracts, & Related Standards

AB-Human Serum (heat-inactivated)
Catalog: 34005-100
Size: 100 ml

PFHM-II Protein-Free Hybridoma Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Hybridoma Media

Gibco® Protein Free Hybridoma Medium II (PFHM II) is a serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® PFHM II features: • Ability to support suspension and stationary hybridomas and myelomas • Complete, ready-to-use formulation • Serum-free, protein-free formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-depende...
Catalog: 23600042
Size: 1 x 10L

Dispase II, powder

Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Other Cell Dissociation Reagents

Dispase II (neutral protease) is an amino-endo peptidase that hydrolyses N-terminal peptide bonds of non-polar amino acid residues. This sequence is found in high frequency in collagen. Dispase II has a mild proteolytic action that makes it useful for the isolation and routine passaging of primary cells. It is relatively gentle, dissociates well at physiological temperature and pH, and generally maintains cell membrane integrity. Gibco® Dispase II is produced in Bacillus polymyxa and packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation and to prevent cl...
Catalog: 17105-041
Size: 5 g

SuperScript® II Reverse Transcriptase

Category: Products & Services > Proteins, Enzymes, & Peptides > Enzymes > Reverse Transcriptase

SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics. • Yield – Reduced RNase H activity results in greater first-strand cDNA yields • Size of ...
Catalog: 18064-071
Size: 4 × 10,000 units

Buffer Dam for XCell SureLock® and XCell II™

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Other Gel Electrophoresis Accessories

Replacement Parts for Electrophoresis Apparatus
Catalog: EI0012
Size: each

XCell SureLock ® Mini-Cell and XCell II™ Blot Module

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Blotting Systems

The XCell line of mini-cells has always distinguished itself by being simple, sturdy, and convenient. The tradition continues with the patented XCell SureLock ® Mini-Cell. The incorporation of a gel tension wedge in place of the rear wedge used on earlier models makes the XCell SureLock ® the easiest-to-use mini-cell on the market. How the System Works The XCell SureLock ® Mini-Cell holds gels firmly with a simple gel tension wedge. When the lever on the gel tension wedge is pushed forward into the locked position, an even horizontal force is generated. This seals the gel/buffer core assembly ...
Catalog: EI0002
Size: 1 unit

TaqMan® Universal Master Mix II, no UNG Mini-Pack (1 x 1 mL)

Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits

TaqMan® Universal Master Mix II is a convenient 2X mix for use in all real-time application including microRNA, gene expression and genotyping. TaqMan® Universal Master Mix II is a convenient 2X mix that includes: -AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure), a highly purified thermostable DNA polymerase, that is hot start-enabled for convenient reaction setup at room temperature -Available in two formats: with and without uracil-DNA glycosylase (UDG) and dUTP⁄dTTP mix to minimize carryover PCR contamination -Optimized salt, dNTP, and buffer concentrations for reliable performance -Passive ...
Catalog: 4440043
Size: 40 reactions

AB-TUBE-ANTI--MYC

Category: Products & Services > Slide & Chip Arrays

AB-TUBE-ANTI--MYC
Catalog: 460603
Size: 1 each

Ion Total RNA-Seq Kit for AB Library Builder™ System

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion Total RNA-Seq Kit for AB Library Builder™ System combines optimized reagents and protocols for automated preparation of representative cDNA libraries for strand-specific RNA sequencing of all types of RNA species on the Ion Personal Genome Machine (PGM™) and Proton™ sequencers. Either small RNA (such as microRNA) or whole transcriptome RNA samples can be prepared for next-generation sequencing. The Ion Total RNA-Seq Kit for AB Library Builder™ System contains the same performance advantages as the manual Ion Total RNA-Seq Kit v2 with the convenience of protocol automation. The gel size...
Catalog: 4482416
Size: 13 reactions

Ion Total RNA Library Protocol Card for AB Library Builder™ System

Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Sequencing & Genetic Analyzer Instrument Accessories

The Ion Total RNA Library Protocol Card for AB Library Builder™System is required for running the Ion Total RNA-Seq Kit for AB for Library Builder™System . This protocol card contains the firmware needed to easily automate the creation of up to 13 whole transcriptome or small RNA (miRNA) libraries upstream of semiconductor sequencing on either the Ion PGM™ or Ion Proton™ platforms. The Ion Total RNA Library Protocol Card for AB Library Builder™System: • Enables whole transcriptome or small RNA (miRNA) library construction on the AB Library Builder™System • Eliminates the need for instrument pr...
Catalog: 4482563
Size: 1 card

PRKCB2 (PKC beta II) Recombinant Human Protein

Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases

The PKC family of serine⁄threonine kinases, including PRKCB2 (PKC beta II), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
Catalog: PR1461C
Size: 100 µg