Media Products

Custom Media: 2-MERCAPTOETHANOL

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media

Custom Media: 2-MERCAPTOETHANOL
Catalog: A13558SA
Size: 1 each

Custom Media: MAGNESIUM CHLORIDE ANHYDROUS

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media

Custom Media: MAGNESIUM CHLORIDE ANHYDROUS
Catalog: A13735IN
Size: 1 each

Glutathione Agarose, Linked Through Sulfur, sedimented bead suspension

Category: Products & Services > Columns, Resins & Plates > Chromatographic Columns & Media > Affinity Media & Standards

Glutathione agarose prepared by linking the sulfur atom of glutathione to crosslinked beaded agarose can be used for purification of GST fusion proteins. Each milliliter of gel can bind approximately 5-6 mg of boivine-liver GST. Adding excess free glutathione liberates the GST fragment from the matrix which can then be regenerated by washing with a high-salt buffer.
Catalog: G2879
Size: 10 ml

Gibco® FreeStyle™ 293 Expression Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > 293 Media

FreeStyle™ 293 Expression Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required. Gibco® FreeStyle™ 293 Expression Medium features: • Complete and ready-to-use format containing GlutaMAX™ supplement • Ability to support suspension growth and transfection of 293-F cells • Animal origin-free, protein-free and chemically defined formulation • Easy use as part of the FreeStyle™ 293 ...
Catalog: 12338-018
Size: 1000 ml

MEM, NEAA, no Glutamine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • NEAA • L-glutamine • Phenol Red • HEPES
Catalog: 10370-088
Size: 10 × 500 ml

DMEM/F-12, HEPES

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM⁄F-12 Media

Dulbecco's Modified Eagle Medium : Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of Gibco® DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM / F-12 is modified as follows: With • L-glutamine • HEPES • Phenol Red
Catalog: 11330-032
Size: 500 ml

MEM, HEPES, no Glutamine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Minimum Essential Media (MEM)

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • HEPES • L-glutamine • Phenol Red
Catalog: 12360-038
Size: 500 ml

Opti-MEM® Reduced Serum Medium

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Opti-MEM® I

Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications. This Opti-MEM® is modified ...
Catalog: 31985-070
Size: 500 ml

Defined Keratinocyte-SFM (1X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Keratinocyte-SFM

Defined Keratinocyte-SFM is a serum-free medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers (1,2). The growth-promoting activity of BPE has been replaced by the inclusion of growth promoting agents which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration ( Note: This formulation contains s...
Catalog: 10744-019
Size: 500 ml

DMEM, High Glucose, no Glutamine, no methionine, no cystine

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES • L-methio...
Catalog: 21013-024
Size: 500 ml

VP-SFM AGT™

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Virus Production Media

VP-SFM AGT™ is a serum free, ultra low protein medium (5 µg/ml) in a new easy-to-use granular format. VP-SFM AGT™ is designed for the growth of VERO cells for virus production. It is also suitable for the growth of COS-7, MDCK, BHK-21 and Hep2 cells and for recombinant protein production.
Catalog: 12559-019
Size: 10 L

DMEM, Low Glucose, GlutaMAX™, Pyruvate

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • Low Glucose • HEPES • Sodium Pyruvate • GlutaMAX™ • Phenol Red
Catalog: 10567-014
Size: 500 ml

IMDM, Powder

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Iscove's (IMDM)

Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. Life Technologies offers a variety of Gibco® IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This IMDM is modified as follows: With Without • L-glutamine • α-thioglycerol • Phenol Red • 2-mercaptoethanol
Catalog: 12200-036
Size: 10 × 1 L

Ham's F-12K (Kaighn's)

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > F-12 Nutrient Mixture

Ham's F-12K (Kaighn's) medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) medium was developed for primary human hepatocytes, as well as some rat and chicken liver cells in a reduced serum environment. This F-12K (Kaighn's) medium is manufactured as follows: With Without • L-glutamine • HEPES • Phenol Red
Catalog: 21127-030
Size: 10 × 500 ml

CD CHO Medium (1X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > CHO & DG44 Media

CD CHO medium is a protein-free, serum-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. With a proven track record of quality for more than 10 years, CD CHO medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates. When to Use CD CHO Medium • Your GS CHO cells are recommended to be grown in CD CHO medium • Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a reg...
Catalog: 10743-029
Size: 1,000 ml

StemPro®-34 SFM (1X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Serum-Free & Specialty Media > Stem Cell Media

StemPro®-34 SFM is a serum-free medium specifically formulated to support the development of human hematopoietic cells in culture (1). StemPro®-34 SFM can be used to study hematopoietic cells from bone marrow, peripheral blood, or neonatal cord blood. StemPro®-34 SFM is performance-tested on normal human bone marrow. It is manufactured with components of human, recombinant or synthetic origin. StemPro®-34 SFM consists of a basal liquid medium and frozen supplement (StemPro®-Nutrient Supplement) which is added at the time of use.
Catalog: 10639-011
Size: 500 ml

MEM, 2X Temin's, no Phenol Red

Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Modified Eagle Medium (MEM)

Temin's Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This MEM is modified as follows: With Without • L-glutamine • HEPES • Earle's salts • Phenol Red • Sodium Bicarbonate
Catalog: 11935-046
Size: 500 ml