Mate-Paired Library Preparations
The Ambion® RNA-Seq Library Construction Kit compatible with Illumina® GAII is a single kit for RNA library construction of either whole transcriptome or small RNA (such as miRNA), for use in downstream next generation sequencing analysis. Designed as a complete solution with a common workflow, the kit optimally combines all necessary reagents for discovery of coding RNA, non-coding RNA, alternative splicing, small RNAs and isoforms, while preserving strand orientation. The Ambion® RNA-Seq Library Construction Kit compatible with Illumina® GAII platform is based on the same technology used in ...
Catalog: 4454073
Size: 1 kit
Size: 1 kit
The Ion Xpress™ Plus Fragment Library Kit provides a rapid and flexible enzymatic-based library construction methodology for the Personal Genome Machine™ sequencing system workflow. Employing enhanced proprietary Ion Shear™ DNA fragmentation chemistry, this revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries, removing the need for a physical shearing device to save time and cost. With significantly higher yields and lower bias than other library construction techniques, superior coverage uniformity is attained with as little as 100...
Catalog: 4471269
Size: 10 reactions
Size: 10 reactions
The Ion Total RNA-Seq Kit for AB Library Builder™ System combines optimized reagents and protocols for automated preparation of representative cDNA libraries for strand-specific RNA sequencing of all types of RNA species on the Ion Personal Genome Machine (PGM™) and Proton™ sequencers. Either small RNA (such as microRNA) or whole transcriptome RNA samples can be prepared for next-generation sequencing. The Ion Total RNA-Seq Kit for AB Library Builder™ System contains the same performance advantages as the manual Ion Total RNA-Seq Kit v2 with the convenience of protocol automation. The gel size...
Catalog: 4482416
Size: 13 reactions
Size: 13 reactions
The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482335
Size: 8 reactions
Size: 8 reactions
The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482340
Size: 96 reactions
Size: 96 reactions
The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482752
Size: 384 reactions
Size: 384 reactions
The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library dilutions for library normalization as required for any next generation sequencing workflow. The Ion Library Equalizer™ Kit helps reduce labor, reagent costs, and further simplifies the Ion semiconductor sequencing workflow for high sample-throughput or barcoded projects, as well as single or low sample number projects. The kit workflow consists of three simple steps: 1) Library amplification with Equalizer™ Primers 2) Library capture onto Equalizer™ Beads 3) He...
Catalog: 4482298
Size: 96 reactions
Size: 96 reactions
The Ion Xpress™ Barcode Adapters 1-16 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Ba...
Catalog: 4471250
Size: 1 kit
Size: 1 kit
The Ion AmpliSeq™ Comprehensive Cancer Panel provides highly multiplexed target selection of genes implicated in cancer research. Encompassing over 50% of the Wellcome Trust Sanger Institute Cancer Gene Census, this is the most comprehensive cancer gene panel available. With all-exon coverage of 409 genes, the Ion AmpliSeq™ Comprehensive Cancer Panel delivers fast, FFPE-compatible, target selection for a broad survey of key genes for semiconductor sequencing. Download the Ion AmpliSeq™ Comprehensive Cancer Panel list of target genes (.csv) . The Ion AmpliSeq™ technology allows ultra-high multi...
Catalog: 4477685
Size: 8 reactions
Size: 8 reactions
The Ion AmpliSeq™ RNA Apoptosis Panel is a screening tool designed against 267 genes involved in the cellular apoptosis pathway. Included are genes associated with death receptor-mediated apoptosis, c-Myc, and p53-mediated apoptosis. • Measure gene expression fold changes between samples • Amplicons are designed to be most inclusive for transcripts of the target gene • Each reaction requires only 500 pg of unfixed RNA or 5 ng of fixed (FFPE) RNA • Prepare amplicon libraries with the Ion AmpliSeq™ RNA Library Kit and sequence on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system This...
Catalog: 4482571
Size: 24 reactions
Size: 24 reactions
The Ion AmpliSeq™ RNA Cancer Panel is a screening tool designed against 50 oncogenes/tumor suppressor genes found in the COSMIC database. This panel has been developed as an RNA complement to the DNA-focused Ion AmpliSeq™ Cancer Hotspot Panel v2. The panel is a single pool of primers representing 50 oncogenes and tumor suppressor genes with coverage of KRAS, BRAF, and EGFR genes. • Measure gene expression fold changes between samples • Amplicons are designed to be most inclusive for transcripts of the target gene • Each reaction requires only 500 pg of unfixed RNA or 5 ng of fixed (FFPE) RNA •...
Catalog: 4482572
Size: 24 reactions
Size: 24 reactions
This Antibody Pair Kit comes with matched antibody pairs to detect and quantify protein levels of rat IL-6. Also available is Buffer Kit for Antibody Pairs that saves time with premade, easy-to-use buffers and solutions optimized for use with Invitrogen Antibody Pairs. The Assay Buffer can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Buffer reagents needed to complete the reaction are sold separately under Catalog No. CNB0011.
Catalog: CRC0063
Size: 10 plates
Size: 10 plates
For the first time, you can obtain the benefits of Ambion® Silencer ® Select siRNAs in a convenient pre-plated, library format. These siRNA collections enable medium and high throughput analysis of phenotypes resulting from inhibition of large sets of targets from specific functional classes. • Ready-to-ship collections of Ambion® Silencer ® Select siRNAs to popular human gene classes • Three individual siRNAs per target provided in a convenient 96-well format • Designed with an all new algorithm that yields better, more consistent knockdown • Novel chemical modifications reduce off-target eff...
Catalog: 4397918
Size: 1 set
Size: 1 set
The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step. PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous ...
Catalog: 4318930
Size: 1 kit
Size: 1 kit
The Ion Library Quantitation Kit is an easy-to-use, flexible solution for the accurate quantitation of library input for the Ion Torrent semiconductor sequencing workflow. This comprehensive kit provides a TaqMan® qPCR mix and library standard to detect and quantify femtomolar amounts of Ion Fragment Libraries. With universal cycling conditions that allow both fast and standard cycling, the Ion Library Quantitation kit supports most real-time platforms. This universal kit provides greater accuracy and specificity compared to non-probe-based methods, delivering simplicity and speed to the PGM™ ...
Catalog: 4468802
Size: 250 reactions
Size: 250 reactions
Library Efficiency® DH5α™ Competent Cells are a versatile strain of chemically competent cells that demonstrate transformation efficiencies of >1 x 10 8 cfu/ µg plasmid DNA. Library Efficiency® DH5α™ Competent Cells are ideal for difficult cloning constructions or any application that uses limiting amounts of DNA. Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal Maintains plasmid DNA with minimum recombination events Delivers high yield plasmid preparations for downstream applications M13mp cloning vectors may be propagated on a lawn of DH5α-FT™ , DH5αF...
Catalog: 18263-012
Size: 1 ml
Size: 1 ml
The Ion Total RNA Library Protocol Card for AB Library Builder™System is required for running the Ion Total RNA-Seq Kit for AB for Library Builder™System . This protocol card contains the firmware needed to easily automate the creation of up to 13 whole transcriptome or small RNA (miRNA) libraries upstream of semiconductor sequencing on either the Ion PGM™ or Ion Proton™ platforms. The Ion Total RNA Library Protocol Card for AB Library Builder™System: • Enables whole transcriptome or small RNA (miRNA) library construction on the AB Library Builder™System • Eliminates the need for instrument pr...
Catalog: 4482563
Size: 1 card
Size: 1 card
The resDNASEQ® MDCK Residual DNA Quantitation System is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of MDCK cell DNA, typically in less than 4 hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high...
Catalog: 4464337
Size: 100 reactions
Size: 100 reactions
The resDNASEQ® NS0 Residual DNA Quantitation System is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from NS0 (murine myeloma) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of NS0 cell DNA, typically in less than 4 hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high m...
Catalog: 4460364
Size: 100 reactions
Size: 100 reactions
The resDNASEQ® Pichia pastoris Residual DNA Quantitation System is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Pichia pastoris cells, an expression system commonly used for the production of recombinant proteins. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 15 pg DNA/mL test sample) and specific quantitation of Pichia pastoris cell DNA, typically in less than four hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—w...
Catalog: 4464338
Size: 100 reactions
Size: 100 reactions
Mate-Paired Library Preparations
Previous: Mate-Paired Library Construction Reagents | Next: Personal Genome Machine (Pgm™) Sequencer
View products by:
Title |
Brand |
Price |
Popularity |
Best Sellers
View topsellers by:
Title |
Brand |
Price |
Popularity |
Favourites
Popular items by:
Popularity |
Our Choices |
All-Round Favourites |
Title |
Popularity |
Our Choices |
All-Round Favourites |
Title
Previous: Mate-Paired Library Construction Reagents | Next: Personal Genome Machine (Pgm™) Sequencer
