Mate-Paired Library Construction Reagents
The Ambion® RNA-Seq Library Construction Kit compatible with Illumina® GAII is a single kit for RNA library construction of either whole transcriptome or small RNA (such as miRNA), for use in downstream next generation sequencing analysis. Designed as a complete solution with a common workflow, the kit optimally combines all necessary reagents for discovery of coding RNA, non-coding RNA, alternative splicing, small RNAs and isoforms, while preserving strand orientation. The Ambion® RNA-Seq Library Construction Kit compatible with Illumina® GAII platform is based on the same technology used in ...
Catalog: 4454073
Size: 1 kit
Size: 1 kit
The 5500 W Paired-End Reagents Kit is used for paired-end sequencing runs for ligation sequencing of the F5 Tag. This kit contains sufficient enzyme, dNTPs, and buffers for 6 lanes. In addition to 5500 W Run Cycle Buffers and 5500 W Buffer D (Reset), this kit requires the use of 5500 W Buffer H.
Catalog: 4475670
Size: 1 kit
Size: 1 kit
This Antibody Pair Kit comes with matched antibody pairs to detect and quantify protein levels of rat IL-6. Also available is Buffer Kit for Antibody Pairs that saves time with premade, easy-to-use buffers and solutions optimized for use with Invitrogen Antibody Pairs. The Assay Buffer can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Buffer reagents needed to complete the reaction are sold separately under Catalog No. CNB0011.
Catalog: CRC0063
Size: 10 plates
Size: 10 plates
The Ion Xpress™ Plus Fragment Library Kit provides a rapid and flexible enzymatic-based library construction methodology for the Personal Genome Machine™ sequencing system workflow. Employing enhanced proprietary Ion Shear™ DNA fragmentation chemistry, this revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries, removing the need for a physical shearing device to save time and cost. With significantly higher yields and lower bias than other library construction techniques, superior coverage uniformity is attained with as little as 100...
Catalog: 4471269
Size: 10 reactions
Size: 10 reactions
The Ion Total RNA-Seq Kit for AB Library Builder™ System combines optimized reagents and protocols for automated preparation of representative cDNA libraries for strand-specific RNA sequencing of all types of RNA species on the Ion Personal Genome Machine (PGM™) and Proton™ sequencers. Either small RNA (such as microRNA) or whole transcriptome RNA samples can be prepared for next-generation sequencing. The Ion Total RNA-Seq Kit for AB Library Builder™ System contains the same performance advantages as the manual Ion Total RNA-Seq Kit v2 with the convenience of protocol automation. The gel size...
Catalog: 4482416
Size: 13 reactions
Size: 13 reactions
The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482335
Size: 8 reactions
Size: 8 reactions
The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482340
Size: 96 reactions
Size: 96 reactions
The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482752
Size: 384 reactions
Size: 384 reactions
The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library dilutions for library normalization as required for any next generation sequencing workflow. The Ion Library Equalizer™ Kit helps reduce labor, reagent costs, and further simplifies the Ion semiconductor sequencing workflow for high sample-throughput or barcoded projects, as well as single or low sample number projects. The kit workflow consists of three simple steps: 1) Library amplification with Equalizer™ Primers 2) Library capture onto Equalizer™ Beads 3) He...
Catalog: 4482298
Size: 96 reactions
Size: 96 reactions
The FreeStyle™ MAX System is a breakthrough technology for rapid and high-yield mammalian protein production. This new mammalian expression technology is available in two convenient formats. FreeStyle™ MAX Reagent is a novel cationic lipid-based reagent designed to transfect FreeStyle™ cells with high efficiency. The FreeStyle™ MAX Expression System combines GIBCO® FreeStyle™ Media, FreeStyle™ MAX Reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293 cells. The only equipment required to use the FreeStyle™ MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinne...
Catalog: 16447-500
Size: 15 ml
Size: 15 ml
Lipofectamine® LTX Reagent offers a new, advanced solution for gene expression studies in hard-to-transfect cells. No other plasmid DNA-specific transfection reagent can match the efficiency, convenience, and gentleness of Lipofectamine® LTX Reagent for transfection of primary, difficult-to-transfect, and sensitive cell lines. Lipofectamine® LTX Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in experiments or cell lines. A certificate of origin may be obtained by contacting Technical Support. ...
Catalog: A12621
Size: 0.1 ml
Size: 0.1 ml
PLUS™ Reagent is used in conjunction with transfection reagents, such as Lipofectamine™, to enhance transfection in adherent cell lines. Some customers find when using Plus™ Reagent that less optimization of DNA concentration is needed to achieve high transfection performance. The addition of Plus™ Reagent may also reduce the quantities of DNA and lipid required by approximately one-half compared to lipid alone. PLUS™ Reagent was specifically designed to expand the array of transfectable cell types (1). PLUS™ Reagent used with Lipofectamine™ Reagent has been demonstrated to improve the transfe...
Catalog: 11514-015
Size: 0.85 ml
Size: 0.85 ml
A complete, ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. • Simple—A single reagent for isolation of DNA-free RNA, RNA-free DNA, and protein • Productive—Obtain higher yields than with traditional guanidine thiocyanate or cesium chloride methods • Versatile—Works with many sample sources, including human, plant, yeast, bacterial, and viral samples • Flexible—Easily scalable depending on starting material and desired yield • Now incorporated into our RiboPure™ and MagMAX™-96 for Microarrays RNA Is...
Catalog: AM9738
Size: 100 ml
Size: 100 ml
RNase AWAY® Reagent is a ready-to-use solution for eliminating RNase and DNA contamination from labware. Apply it evenly over the surface of glassware or plasticware to be treated and then rinse it away with distilled water. Unwanted RNase and DNA contamination are eliminated. RNase AWAY® Reagent is nonabrasive, noncarcinogenic, and nonbiologically corrosive. Performance and Quality Testing: RNase AWAY® Reagent is functionally tested for the elimination of RNase activity and DNA. No detectable RNase activity or DNA is observed.
Catalog: 10328-011
Size: 250 ml
Size: 250 ml
Gibco® Zeocin™ selective reagent is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus . Gibco® Zeocin™ selective reagent acts by binding to and cleaving DNA, leading to cell death. Gibco® Zeocin™ selective reagent is used for selection of yeast, bacterial and mammalian cell cultures. This product is supplied at 100 mg/ml in sterile water and is used at a working concentration ranging between 50 - 400 µg/ml depending on the cell type. Life Technologies offers a variety of antibiotics and antimycotics for cell culture applications. Product Use For Res...
Catalog: R250-05
Size: 50 ml
Size: 50 ml
This Applied Biosystems® kit provides the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, and oligo (dT) 16 , reverse primers are included for cDNA synthesis flexibility. Supplied as 2000 reactions in 10 tubes (200 reactions/tube). • Contains MultiScribe® Reverse Transcriptase, a recombinant Moloney Murine Leukemia Virus Reverse Transcriptase • Random hexamers and oligo (dT) 16 included for flexibility • Individual components provide versatility in assay set-up • Compatible with one-step or two-step RT-PCR reactions TaqMan® Reverse Transcription Reagents provide all...
Catalog: 4304134
Size: 2000 reactions
Size: 2000 reactions
The PrepMan® Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions. These samples often have high lipid content that can inhibit PCR amplification of the template. Using a simple boil and spin protocol, PrepMan®Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step. PrepMan® Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous ...
Catalog: 4318930
Size: 1 kit
Size: 1 kit
Lipofectamine® RNAiMAX Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine® RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types. With Lipofectamine® RNAiMAX Transfection Reagent you will get: Superior transfection efficiency requiring lower RNAi concentrations leading to more effective gene knockdown with minimal non-specific effects Easy optimization due to minimal cytotoxicity across a 10-f...
Catalog: 13778-100
Size: 0.1 ml
Size: 0.1 ml
StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell su...
Catalog: A11105-01
Size: 100 ml
Size: 100 ml
For the first time, you can obtain the benefits of Ambion® Silencer ® Select siRNAs in a convenient pre-plated, library format. These siRNA collections enable medium and high throughput analysis of phenotypes resulting from inhibition of large sets of targets from specific functional classes. • Ready-to-ship collections of Ambion® Silencer ® Select siRNAs to popular human gene classes • Three individual siRNAs per target provided in a convenient 96-well format • Designed with an all new algorithm that yields better, more consistent knockdown • Novel chemical modifications reduce off-target eff...
Catalog: 4397918
Size: 1 set
Size: 1 set
Mate-Paired Library Construction Reagents
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