Lysis Buffert Western
These 2 buffers are to be used only as a complement to the SuperScript® III Platinum® CellsDirect Kits when large number of samples are processed. See manual of SuperScript® III Platinum® CellsDirect Kits for protocol recommendations.
Catalog: 11739-010
Size: 1 kit
Size: 1 kit
The same lysis buffer found in the Ambion® Cells-to-Signal™ Kit. Supplied in 5 bottles containing 10 mL each.
Catalog: AM8728
Size: 5 bottle
Size: 5 bottle
The MagicMark™ XP Western Protein Standard is specifically designed for easy and convenient protein molecular weight estimation directly on western blots. The standard consists of 9 recombinant proteins each of which contains an IgG binding site. The IgG binding site binds the primary or secondary antibody used for detection of the target protein allowing direct visualization of the standard on the western blot. The MagicMark™ XP Standard is compatible with all western kits and substrates (chemiluminescent, chromogenic and fluorescent) and offers: • Direct visualization of molecular weight sta...
Catalog: LC5602
Size: 250 µl
Size: 250 µl
NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay (Luminex), ELISA, and Western blotting.
Catalog: FNN0021
Size: 100 ml
Size: 100 ml
The Protein Quant Sample Lysis Kit contains the reagents required for the preparation of target and control cell lysates for use with TaqMan® Protein Assay experiments. It contains a one-step lysis solution for preparing crude cell lysates from cultured cells, and the lysates are compatible with cell counting as well as total protein quant measurements such as a microBCA assay. The Protein Quant Sample Lysis Kit contains a buffered nonionic detergent solution for the gentle lysis of adherent and non-adherent cultured cells under non-denaturing conditions
Catalog: 4448536
Size: 25 ml
Size: 25 ml
The Bolt® Western Pack (Nitrocellulose) includes two Bolt® Mini Blot Modules and all of the buffers and pre-cut membrane/filter paper sandwiches you will need for the wet transfer of proteins from Bolt® Bis-Tris Plus gels to nitrocellulose membranes. Each pack contains: • Bolt® Mini Blot Modules • Bolt® Transfer Buffer (20X) • Bolt® Antioxidant • Nitrocellulose pre-cut membranes (0.2 µm) About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total ...
Catalog: B1000A
Size: 1 kit
Size: 1 kit
The Bolt® Western Pack (PVDF) includes two Bolt® Mini Blot Modules and all of the buffers and pre-cut membrane/filter paper sandwiches you will need for the wet transfer of proteins from Bolt® Bis-Tris Plus gels to PVDF membranes. Each pack contains: • Bolt® Mini Blot Modules • Bolt® Transfer Buffer (20X) • Bolt® Antioxidant • PVDF pre-cut membranes (0.2 µm) About the Bolt® Mini Blot Module The Bolt® Mini Blot Module is a wet transfer device used exclusively with the Bolt® Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. ...
Catalog: B1000B
Size: 1 kit
Size: 1 kit
Cell extraction buffer suitable for use in ELISA and Western blotting.
Catalog: FNN0011
Size: 100 ml
Size: 100 ml
Invitrogen Cal-Lyse™ is a lysing solution formulated to enable the lysis of erythrocytes in samples of anticoagulated human peripheral blood. Invitrogen Cal-Lyse™ lysing solution is intended to be used as an aid in the enumeration of leukocytes that have been stained with monoclonal antibodies for flow cytometric analysis
Catalog: GAS-010S-100
Size: 100 ml
Size: 100 ml
High-Yield Lyse is a premixed, fixative-free erythrocyte lysing solution that can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with Invitrogen's fluorochrome-conjugated monoclonal antibodies.
Catalog: HYL-250
Size: 500 ml
Size: 500 ml
Gibco® ACK (Ammonium-Chloride-Potassium) Lysing Buffer is suitable for use in the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats and bone marrow. The complete formulation and lysis protocol are available in our Technical Reference Library. cGMP Manufacturing and Quality System Gibco® ACK Lysing Buffer is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.
Catalog: A10492-01
Size: 100 ml
Size: 100 ml
Total Exosome Isolation (from cell culture media) enables fast and efficient enrichment of intact exosomes from cell culture media samples. • Maximize recovery of intact exosomes for any type of downstream application • Easily isolate exosomes using our simple and reliable protocol • Avoid time-consuming ultra-centrifugation • Maximize flexibility—can be scaled up or down depending on the sample size Isolation of exosomes can be a tedious, non-specific, and difficult process. Total Exosome Isolation (from cell culture media) enables simple and reliable concentration of intact exosomes from cel...
Catalog: 4478359
Size: 50 ml
Size: 50 ml
Total Exosome Isolation (from serum) enables fast and efficient enrichment of intact exosomes from blood serum samples. • Maximise recovery of intact exosomes for any type of downstream applications • Easily isolate exosomes with our simple and reliable protocol • Avoid time-consuming ultra-centrifugation • Maximize flexibility—can be scaled up or down depending on the sample size Isolation of exosomes can be a tedious, non-specific, and difficult process. Total Exosome Isolation (from serum) enables simple and reliable concentration of intact exosomes from blood serum samples with a protocol ...
Catalog: 4478360
Size: 6 ml
Size: 6 ml
Cells-to-C T Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan® Gene Expression Cells-to-C T ™ Kit (20X RT enzyme mix and 20X RT buffer). These reagents are sufficient for 2500 RT reactions. When combined with the Cells-to- C T Bulk Lysis Reagents , these reagents can be easily incorporated into automated, high throughput applications.
Catalog: 4391852C
Size: 2500 reactions
Size: 2500 reactions
This is the binding buffer used with MagVet™ Mycobacterium paratuberculosis Isolation Kit, validated for DNA extraction.
Catalog: MBL2
Size: 125 mL
Size: 125 mL
The DNA Extract All Reagents Kit provides PCR-ready DNA from a wide variety of sample types, ranging from blood to buccal swabs to plant tissues in 5 minutes. Simply mix your sample with the DNA lysis solution provided. Incubate for 3 minutes and add the DNA stabilizing solution, also provided. After extraction, robust PCR amplification can be completed using TaqMan® GTXpress™ Master Mix or TaqMan® OpenArray® Genotyping Master Mix (pre-amplified sample only), depending on your application. PCR can be performed using either Fast or Standard mode thermal cycling conditions. The DNA Extract All R...
Catalog: 4402599
Size: 200 mL
Size: 200 mL
This purified Mouse Histidine (C-term) Tag Monoclonal Antibody recognizes a polyhistidine amino acid sequence at the carboxy-terminus of proteins. The free carboxy-terminus of the terminal histidine residue is a key element of the epitope recognition site of the anti-His (C-term) Antibody. The Anti-His(C-term) Monoclonal Antibody recognizes the sequence: -His-His-His-His-His-His-COOH . Validated applications for Histidine (C-term) Tag monoclonal antibody are western blotting and ELISA. Antibody Specifications: • Applications: Validated applications for Histidine (C-term) Tag Monoclonal Antibod...
Catalog: R931-25
Size: 50 µl
Size: 50 µl
Goat whole molecule anti-human immunoglobulin Format/Formulation: HRP Conjugate
Catalog: AHI0704
Size: 0.7 mg⁄ml
Size: 0.7 mg⁄ml
This purified Mouse anti-ErbB-2 Monoclonal Antibody is specific to human, rat, and mouse ErbB2 (HER2). Over-expression of c-erbB-2 is observed in 15–40% of primary human breast tumors and correlates with poor prognosis in node positive breast cancer. Over-expression of c-erbB-2 is also observed in ovarian, gastric, salivary, and non-small cell lung carcinomas. Validated applications for mouse anti-ErbB-2 monoclonal antibody are immunoprecipitation, immunohistochemistry, flow cytometry, and Western blotting. Antibody Attributes: • Applications: Validated applications for Mouse anti-ErbB-2 monoc...
Catalog: AHO1011
Size: 100 µg
Size: 100 µg
Goat whole molecule anti-human immunoglobulin Format/Formulation: HRP Conjugate
Catalog: AHI0304
Size: 0.7 mg⁄ml
Size: 0.7 mg⁄ml
Lysis Buffert Western
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