Luciferase Substrates

Silencer ® Firefly Luciferase (GL2 + GL3) siRNA (inc. 2 nmol Neg. Control siRNA)

Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Positive Controls

Ambion® Silencer ® Firefly Luciferase (GL2 + GL3) Positive Control siRNA is ideal for developing and optimizing siRNA experiments. The control is validated for use in human cell lines and optimized for mouse and rat. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to...

Catalog: AM4629
Size: 5 nmol

Luciferase Monoclonal Antibody, Mouse (clone CS 17)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Epitope Tag & Reporter Specific Antibodies

This mouse anti-Luciferase monoclonal antibody is specific to mouse Luciferase protein. Luciferase is a 61 kD enzyme that catalyzes the reaction of luciferin to generate light after its activation by ATP and oxidative decarboxylation Validated applications for Luciferase monoclonal antibody are Western blotting, immunofluorescence, and immunohistochemistry (frozen tissue). Applications: validated Western blotting, immunofluorescence, and immunohistochemistry (frozen tissue) Host species and isotype: mouse IgG1 Clone ID of monoclonal antibody (mAb): CS 17 Reactivity: purified Luciferase in mous...

Catalog: 35-6700
Size: 100 µg

TMB Solution (ELISA)

Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Chromogens

TMB (Tetramethyl Benzidine) chromogen solution may be used as a substrate for horseradish peroxidase (HRP) in ELISAs.

Catalog: 00-2023
Size: 500 ml

Histone H3 Peptide (1-20)

Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Kinase & Phosphatase Substrates

Matched and validated lipid and peptide substrates are available to complement the Adapta® Universal Kinase Assay. The assays developed with these reagents allow for primary or secondary screening of kinase inhibitors.

Catalog: PV5707
Size: 1 ml

Luminescence Enhancer For Membrane Blotting Assays, Nitro-Block-II™ Enhancer

Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Chemiluminescent Substrates

This concentrated Nitro-Block-II™ Enhancer can be used with CSPD® or CDP-Star® chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II™ membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF membranes. • Chemically optimized formulations provide enhanced signal intensity in blotting applications with dioxetane-based substrate solutions (CSPD® or CDP-Star® substrates) on nitrocellulose or PVDF membranes. • Enhancer increases signal intensity, allowing decreased signal acquisition time. Increa...

Catalog: T2184
Size: 20 ml

Stable DAB

Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Chromogens

Stable DAB is a stable, pre-diluted chromogen system designed to clearly localize tissue, DNA, mRNA, and antigens in in situ hybridization and immunoperoxidase procedures. Stable DAB is a ready-to-use reagent containing the correct amounts of diaminobenzidine and hydrogen peroxide. Stable DAB does not fade, creating a permanent record of results under a standard coverslip. It is color coded red for easy user identification.

Catalog: 750118
Size: 250 ml

Fluorescein Di-β-D-Galactopyranoside (FDG)

Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Beta-Galactosidase Substrates

Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive substrates for galactosidases. Nonfluorescent FDG is sequentially hydrolyzed by β-galactosidase, first to fluorescein monogalactoside (FMG) and then to highly fluorescent fluorescein. Enzyme-mediated hydorlysis of FDG can be followed by the increase in either absorbance or fluorescence.

Catalog: F-1179
Size: 5 mg

TMB Solution (BLOT)

Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Chromogens

TMB (Tetramethyl Benzidine) chromogen solution may be used with horseradish peroxidase (HRP)-based immunodetection procedures.

Catalog: 00-2019
Size: 500 ml