ER-Tracker Blue-White DPX dye is a photostable probe that is selective for the endoplasmic reticulum (ER) in live cells. The cells can be aldehyde-fixed after staining with the dye but significant fluorescence signal will be lost. With excitation at ~374 nm, the environmentally-sensitive probe exhibits emission between 430 and 640 nm. A long-pass DAPI filter works best.
Size: 20 × 50 µl
The ER-Tracker™ Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY® TR dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K + channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell typ...
Size: 100 µg
Alkaline Phosphatase Live Stain is a stem cell imaging product that allows users to differentially stain pluripotent stem cells (PSCs). The dye is a cell-permeable fluorescent substrate for alkaline phosphatase (AP) that is non-toxic to cells, diffusing out over the course of two hours. The Alkaline Phosphatase Live Stain consists of 50 µL of a 500X fluorescein-based dye in DMSO, sufficient for staining two 24-well plates, four 6 cm dishes or two 10 cm dishes. This Alkaline Phosphatase Live Stain allows you to: • Identify pluripotent stem cells with a strong signal above background • Stain PSC...
Size: 50 µl
A suite of accessories has been designed to enable research on living cells using your ArcturusXT™ LCM Instrument. Researchers may use slides or Petri dishes to perform imaging and microdissection experiments. Following a validated protocol from Arcturus® LCM Systems from Applied Biosystems—the leaders in tissue-based microdissection—the live cells may be isolated, remaining viable for subsequent re-culture or direct processing for use in downstream applications. Polyethylene naphthalate (PEN) Membrane Frame Slides for Live Cell Microdissection • Specially optimized for use with live cell expe...
Size: 25 each
MitoSOX™ Red reagent is a novel fluorogenic dye specifically targeted to mitochondria in live cells. Oxidation of MitoSOX™ Red reagent by superoxide produces red fluorescence. • Readily oxidized by superoxide but not by other ROS- or RNS-generating systems • Absorption/emission maxima: ~510/580 nm • Use for live cell imaging • Rapidly and selectively targeted to the mitochondria Quick, Easy Detection of Mitochondrial Superoxide in Live Cells The production of superoxide by mitochondria can be visualized in fluorescence microscopy using the MitoSOX™ Red reagent. MitoSOX™ Red reagent permeates l...
Size: 10 × 50 µg
The LIVE/DEAD® Fixable Dead Cell Stain Kit employs an amine-reactive fluorescent dye to evaluate mammalian cell viability by flow cytometry. In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity between the live and dead cell populations is typically greater than 50 fold, and this fluorescence intensity discrimination is completely preserved fol...
Size: 1 kit
The LIVE/DEAD® Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. Calcein violet AM and aqua-fluorescent reactive dye are optimal dyes for this application; both dyes utilize the violet laser allowing other laser lines to be used for conventional fluorochromes.
Size: 1 kit
NucRed™ Live 647 ReadyProbes™ Reagent is a cell-permeant nuclear stain that emits bright, far-red fluorescence when bound to DNA. Like other ReadyProbes™ reagents, NucRed™ Live 647 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells. NucRed™ Live 647 ReadyProbes™ Reagent provides: • Exceptionally bright far-red fluorescence well separated from blue, green, and red fluorophores • Rapid staining of live or fixed cells without wash steps • Ready-to-use liquid formulation in convenient dropper ...
Size: 1 kit
Hoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to DNA. With NucBlue® Live ReadyProbes™ Reagent we have formulated this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Features of NucBlue® Live ReadyProbes™ Reagent: Ready-to-use liquid formulation of high-purity Hoechst 33342 No need to dilute, weigh, or pipette Convenient dropper bottle—just use two drops per ml Stable at room temperature—keep handy at your scope or cell cult...
Size: 1 kit