lipid conjugates sphingolipids

Cholera Toxin Subunit B (Recombinant), Alexa Fluor® 647 Conjugate

Category: Products & Services > Labeling & Detection Products > BioConjugates > Protein Conjugates

Molecular Probes® cholera toxin conjugates are made from a recombinant version of the B subunit only. This allows us to provide a very high-purity product that is completely free of the toxic A subunit. Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside G M1 , making it a powerful tool for retrograde labeling of neurons. This tracer has been used in a variety of applications, including tracing of rat forebrain afferents, projections of the parabrachial region, and neurons of the urinary bladder wall. When used in neuronal tracing applications, CT-B is typically introduc...
Catalog: C-34778
Size: 100 µg

Histidine Tag (6xHis) Mouse Monoclonal Antibody (clone 3D5) HRP Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Epitope Tag & Reporter Specific Antibodies

This purified Mouse Histidine (C-term) Tag Monoclonal Antibody recognizes a polyhistidine amino acid sequence at the carboxy-terminus of proteins. The free carboxy-terminus of the terminal histidine residue is a key element of the epitope recognition site of the anti-His (C-term) Antibody. The Anti-His(C-term) Monoclonal Antibody recognizes the sequence: -His-His-His-His-His-His-COOH . Validated applications for Histidine (C-term) Tag monoclonal antibody are western blotting and ELISA. Antibody Specifications: • Applications: Validated applications for Histidine (C-term) Tag Monoclonal Antibod...
Catalog: R931-25
Size: 50 µl

Lectin PHA-L From Phaseolus vulgaris (red kidney bean), Alexa Fluor® 647 Conjugate

Category: Products & Services > Labeling & Detection Products > BioConjugates > Lectins

Lectin phytohemagglutinin-L (PHA-L) is strongly inhibited by N-acetylglucosamine β(1-2) mannopyranosyl residues.[ALTREF PN29369] This lectin has been shown to agglutinate leukocytes and induce mitogenic activity in T-lymphocytes[ALTREF PN29369,PN29368] and has also been used as an anterograde tracer for the study of neuronal pathways.[ALTREF PN20284]
Catalog: L32457
Size: 1 mg

HCS LipidTOX™ Red Neutral Lipid Stain, for cellular imaging

Category: Products & Services > Kits & Assays > Cell Analysis Kits > HCS Detection Kits

The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid sta...
Catalog: H34476
Size: 1 each

IgG Goat Anti-Human pAb, PE Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

The IgG (γ), goat F(ab’), 2 R-phycoerythrin (R-PE) dye conjugate is a polyclonal goat antibody that is Fc specific and recognizes human IgG. R-PE is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence emission without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients. Use of R-PE in antibody conjugates results in probes with greatly enhanced detectability. Antibody Specifications Description: IgG (γ), Goat F(ab’)2 polyclonal, Fc specific Label: R-PE Validated Appl...
Catalog: H10104
Size: 0.5 ml

CD32 Mouse Anti-Human mAb (clone AT10), Fluorescein (FITC) Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

CD32, Mouse Anti-Human, (FITC); FITC conjugated Monoclonal antibody specific to Human CD32. This antibody is validated for use in Flow Cytometry. Anti-CD32 recognizes the expressed product of the FCGR2A gene.
Catalog: CD3201
Size: 0.5 ml

TCR alpha/beta Hamster Anti-Mouse mAb (clone H57-597), Alexa Fluor® 488 Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

TCR ALPHA BETA, Hamster Anti-Mouse, (Alexa Fluor 488); Alexa Fluor 488 conjugated Monoclonal antibody specific to Mouse TCR α/β. This antibody is validated for use in Flow Cytometry. Anti-TCR α/β recognizes the expressed product of the TCRA/B gene.
Catalog: HM3620
Size: 1 ml

Avidin, NeutrAvidin®, Horseradish Peroxidase Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Neutravidin

Our horseradish peroxidase (HRP) conjugate of NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding.
Catalog: A-2664
Size: 1 mg

Streptavidin, Marina Blue® conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin

Our streptavidin, Marina Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ~365/460 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.
Catalog: S-11221
Size: 1 mg

F(ab')2 Fragment of Goat Anti-Rat IgG (H+L), Horseradish Peroxidase Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Affinity purified F(ab')2 gragment of goat anti-rat IgG conjugated to horseradish peroxidase. To minimize cross-reactivity, this antibody has been absorbed against human IgG, human serum, mouse IgG, mouse serum and bovine serum.
Catalog: A10548
Size: 0.5 mg

Lectin PNA From Arachis hypogaea (peanut), Alexa Fluor® 568 Conjugate

Category: Products & Services > Labeling & Detection Products > BioConjugates > Lectins

Lectin PNA is specific for terminal β-galactose. It will agglutinate human erythrocytes, but only after neuraminidase treatment.[ALTREF PN40265]
Catalog: L32458
Size: 1 mg

Phosphotyrosine [pY] Mouse Monoclonal Antibody (clone PY20), AP Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Anti-Phospho Amino Acid Antibodies

Clone/PAD: PY20 Immunogen: Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine Specificity: Reacts specifically with tyrosine phosphorylated proteins in the native or denatured states, and does not react with phosphoserine or phosphothreonine. Reacts with a wide variety of tyrosine-phosphorylated proteins in all tested species. Reactivity: Human, Mouse, (positive controls: mouse brain, NIH 3T3 cells (+/- TPA), K562 cells, EGF-stimulated A431 cells, and MCF-7 cell lysates) Applications: Western blotting, Immunoprecipitation, ELISA, Affinity chromotograp...
Catalog: 03-7722
Size: 100 µg

Annexin V, FITC Conjugate (Replaces AnnexinV01, AnnexinV013, PHN1010, PHN1008)

Category: Products & Services > Labeling & Detection Products > BioConjugates > Annexin V

In collaboration with Nexins Research BV, we provide the best and brightest annexin V conjugates available, including this FITC annexin V conjugate. Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, one of the earliest indicators of apoptosis.
Catalog: A13199
Size: 500 µl

Streptavidin-Fluorescein Isothiocyanate (FITC) conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin

The pcDNA™6 BioEase™ Gateway® Biotinylation System provides an easy, efficient method for expressing, purifying, and detecting biotinylated recombinant proteins. The pcDNA™6/BioEase™-DEST vector (Figure 1) incorporates a 72 amino acid sequence from K. pneumoniae that directs in vivo biotinylation of a specific lysine residue. This K. pneumoniae tag is recognized and efficiently biotinylated in vivo by a native mammalian system similar to the enzyme Biotin Protein Ligase (BPL) in E. coli (1). Proteins produced from the pcDNA™6/BioEase™-DEST vector are expressed as fusion proteins to this sequen...
Catalog: SA100-02
Size: 50 µl

Paclitaxel, BODIPY® 564⁄570 Conjugate (BODIPY® 564⁄570 Taxol)

Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents

The red-orange-fluorescent BODIPY® 564/570 paclitaxel can be used to study microtubules in solution.
Catalog: P7501
Size: 10 µg

Streptavidin-Horseradish Peroxidase (HRP) conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Avidin, Streptavidin & Biotin > Streptavidin

Streptavidin-Horseradish Peroxidase (HRP) conjugate
Catalog: SA100-01
Size: 50 µl

HCS LipidTOX™ Green Neutral Lipid Stain, for cellular imaging

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Green neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid s...
Catalog: H34475
Size: 1 each

Biotin Mouse Antibody (clone Z021), HRP Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Anti-Hapten Antibodies

Clone/PAD: Z021 Immunogen: Synthetic biotin Specificity: Binds strongly to biotinylated proteins. Reactivity: Biotinylated proteins Applications: ELISA, Cell and tissue staining
Catalog: 03-3720
Size: 1 ml

Rabbit Anti-Goat IgG (H+L), Horseradish Peroxidase Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

The rabbit anti—goat IgG, horseradish peroxidase (HRP) conjugate has been adsorbed against human and rat serum proteins prior to labeling to minimize crossreactivity.
Catalog: R-21459
Size: 1 mg

Mouse Anti-Human IgG1, Horseradish Peroxidase Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies

Mouse monoclonal HP6069; specific for the Fc region of human IgG1 and conjugated to horseradish peroxidase. Replaces product number 053320.
Catalog: A-10648
Size: 200 µg