Ligation-Based Sequencing

BigDye® Terminator v3.1 Cycle Sequencing Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337458
Size: 25000 reactions

BigDye® Terminator v3.1 Cycle Sequencing Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337457
Size: 5000 reactions

BigDye® Terminator v3.1 Cycle Sequencing Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337456
Size: 1000 reactions

BigDye® Terminator v1.1 & v3.1 5X Sequencing Buffer

Category: Products & Services > Buffers & Chemicals > Sequencing Buffers & Reagents

This sequencing buffer is optimized for use with BigDye® Terminator v1.1 and v3.1 Cycle Sequencing Kits. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4336701
Size: 233 ml

3500/3500xL Sequencing Standards, BigDye® Terminator v3.1

Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > DNA Sequencing & Matrix Standards

The BigDye® Terminator v3.1 sequencing standard kit contains four tubes, each of which contains enough DNA of a known 1200 base pair sequence prepared with BigDye® Terminator v3.1 to perform one spectral calibration⁄performance check and⁄or one control sequencing run on the Applied Biosystems® 3500 Series Genetic Analyzer. The DNA contained in the preparation has been lyophilized to maximize stability. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Catalog: 4404312
Size: 4 tubes

Sequencing Analysis Software v 5.4 Upgrade from v5.3.1

Category: Products & Services > Instruments, Software, & Accessories > Software > Sequencing & Genetic Analysis Software

This product can only be used to upgrade Sequencing Analysis Software v5.3.1 installed on standalone computers, computers separate to the instrument data collection system. Please talk with your representative to upgrade the data collection system and secondary analysis software running on the data collection computer. Sequencing Analysis Software enables you to basecall, trim, display, edit, and print data from our entire line of capillary DNA sequencing instruments for data analysis and quality control. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4310991
Size: 1 CD

Ion PGM™ Sequencing 400 Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The new Ion PGM™ Sequencing 400 Kit is an integral component of the Personal Genome Machine™ sequencing platform and single-day sequencing workflow, designed to sequence templated Ion Sphere™ particles deposited in Ion 314™ Chip v2. This sequencing kit should be used in conjunction with Ion OneTouch™ 2 template preparation. The Ion PGM™ Sequencing 400 Kit provides you with: • Simple and natural sequencing chemistry • Low-cost standard reagents for lower cost per sample • Sequencing read lengths of 400 base pairs with short run times (up to 8.5 hours) • Support for Ion 314™ Chip v2 • Support fo...
Catalog: 4482002
Size: 4 sequencing runs

Ion PI™ Sequencing 200 Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Sequencing Kits

The new Ion PI™ Sequencing Kit is an integral component of the Ion Proton™ sequencing platform and single-day sequencing workflow, designed to sequence templated Ion PI™ Ion Sphere™ particles deposited in Ion PI™ chips for human exome resequencing or transcriptional profiling. The Ion PI™ Sequencing 200 Kit provides you with: • Simple and natural sequencing chemistry • Low-cost standard reagents for lower cost per sample • Support for Ion PI™ chips • Support for multiplexed samples • Lower cost per run for a variety of applications, including exome and whole transcriptome sequencing The Bencht...
Catalog: 4482283
Size: 8 reactions

3500 Dx Series Sequencing Standard, Big Dye® Terminator v1.1

Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > DNA Sequencing & Matrix Standards

This BigDye® Terminator v1.1 sequencing standard consists of four tubes, each of which contains DNA of a known 1,200 base pair sequence prepared with BigDye® Terminator v1.1. Sufficient DNA is included to perform one spectral calibration⁄performance check and⁄or one control sequencing run on an Applied Biosystems® 3500 Dx Series genetic analyzer. The DNA preparation has been lyophilized to maximize stability.
Catalog: 4462113
Size: 4 tubes

LB Broth Base, Powder (Lennox L Broth Base)®

Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media

Formulation per one liter: 10 g SELECT Peptone 140 5 g SELECT Yeast Extract 5 g sodium chloride
Catalog: 12780-029
Size: 2.5 kg

Luria Broth Base (Miller's LB Broth Base)®, Powder

Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media

Formulation per one liter: 10 g SELECT Peptone 140 5 g SELECT Yeast Extract 10 g sodium chloride
Catalog: 12795-027
Size: 500 g

I-Block™ Protein-Based Blocking Reagent

Category: Products & Services > Buffers & Chemicals > Immunoassay Buffers & Reagents > ELISA Buffers & Reagents

This Casein-based blocking reagent can be used with Western-Light™, Western-Light Plus™, Western-Star™, ELISA-Light™, Southern-Light™, and Southern-Star™ Detection Systems. For Research Use Only. Not for use in diagnostics procedures.
Catalog: T2015
Size: 30 g

TOPO® TA Cloning® Kit for Sequencing, with One Shot® Mach1™ T1 Phage-Resistant Chemically Competent E. coli

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > PCR Cloning Vectors

The TOPO® TA Cloning® Kits for Sequencing provide a highly efficient, 5 minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4-TOPO® TA vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction. These kits include everything necessary to clone and select recombinant vectors containing your PCR fragment of choice. Get More Sequence— Allows for more insert sequence and less vector seuquence when usin...
Catalog: K4530-20
Size: 20 reactions

Site Prep and Safety Guide, Sequence Detection System 7900HT

Category: Products & Services > Literature > Manuals > User Guides, Manuals & Bulletins

This is the Site Prep and Safety Guide for the 7900HT Sequence Detection System. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4317595
Size: 1 unit

Retainer & Base Set (Standard) for 3500 Dx/3500xL Dx Genetic Analyzers, 96-Well

Category: Products & Services > Instruments, Software, & Accessories > Plates & Accessories > Adhesive Film & Other Plate-Related Accessories

This is a 96-well retainer & base set (standard) designed for use with the 3500 Dx⁄3500xL Dx Genetic Analyzer. Septa and plate base⁄retainer kits for 8 tube strip, 96 and 384-well plates used with the 3500 Dx Series Genetic Analyzers. Note: The 3500 Dx Series Systems and consumables are for distribution and use in select countries only.
Catalog: 4410227
Size: 4 sets

Ion Xpress™ Barcode Adapters 1-16 Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion Xpress™ Barcode Adapters 1-16 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Ba...
Catalog: 4471250
Size: 1 kit

Ion Xpress™ Plus Fragment Library Kit

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion Xpress™ Plus Fragment Library Kit provides a rapid and flexible enzymatic-based library construction methodology for the Personal Genome Machine™ sequencing system workflow. Employing enhanced proprietary Ion Shear™ DNA fragmentation chemistry, this revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries, removing the need for a physical shearing device to save time and cost. With significantly higher yields and lower bias than other library construction techniques, superior coverage uniformity is attained with as little as 100...
Catalog: 4471269
Size: 10 reactions

Ion OneTouch™ 200 Template Kit v2

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Templated Bead Preparation Kits & Reagents

The Ion OneTouch™ 200 Template Kit v2 contains template preparation reagents for automated and reproducible template preparation for 200 base-read libraries using the Ion OneTouch™ System . As an integral component of the semiconductor sequencing workflow, the Ion OneTouch™ 200 Template Kit v2 is designed to make high-quality templated Ion Sphere™ particles using the Ion OneTouch™ System to streamline the overall Ion sequencing workflow, yield significant time savings, increase productivity, and accelerate scientific discovery. The Ion OneTouch™ 200 Template Kit v2 provides you with: • Simple ...
Catalog: 4478316
Size: 10 reactions

Ion PGM™ Enrichment Beads

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Templated Bead Preparation Kits & Reagents

Ion PGM™ Enrichment Beads are for use with Ion PGM™ template preparation kits for enrichment of template-positive Ion Sphere™Particles. The Ion PGM™ Enrichment Beads provide you with: • Simple enrichment for template-positive Ion Sphere™ Particles • Dynabeads® technology • Support for Ion PGM™ System workflow • Support for Ion OneTouch™ 200 Template Kit v2, Ion OneTouch™ Template Kit, Ion PGM™ 200 Xpress™ Template Kit, and Ion Xpress™ Template Kit The Ion PGM™ Enrichment Beads are streptavidin-coupled Dynabeads®, the gold standard for isolation due to their high binding affinity and uniformity...
Catalog: 4478525
Size: 10 reactions

Ion AmpliSeq™ Comprehensive Cancer Panel

Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing

The Ion AmpliSeq™ Comprehensive Cancer Panel provides highly multiplexed target selection of genes implicated in cancer research. Encompassing over 50% of the Wellcome Trust Sanger Institute Cancer Gene Census, this is the most comprehensive cancer gene panel available. With all-exon coverage of 409 genes, the Ion AmpliSeq™ Comprehensive Cancer Panel delivers fast, FFPE-compatible, target selection for a broad survey of key genes for semiconductor sequencing. Download the Ion AmpliSeq™ Comprehensive Cancer Panel list of target genes (.csv) . The Ion AmpliSeq™ technology allows ultra-high multi...
Catalog: 4477685
Size: 8 reactions