Library Preparation Kits for Sequencing
The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library dilutions for library normalization as required for any next generation sequencing workflow. The Ion Library Equalizer™ Kit helps reduce labor, reagent costs, and further simplifies the Ion semiconductor sequencing workflow for high sample-throughput or barcoded projects, as well as single or low sample number projects. The kit workflow consists of three simple steps: 1) Library amplification with Equalizer™ Primers 2) Library capture onto Equalizer™ Beads 3) He...
Catalog: 4482298
Size: 96 reactions
Size: 96 reactions
The Ambion® RNA-Seq Library Construction Kit compatible with Illumina® GAII is a single kit for RNA library construction of either whole transcriptome or small RNA (such as miRNA), for use in downstream next generation sequencing analysis. Designed as a complete solution with a common workflow, the kit optimally combines all necessary reagents for discovery of coding RNA, non-coding RNA, alternative splicing, small RNAs and isoforms, while preserving strand orientation. The Ambion® RNA-Seq Library Construction Kit compatible with Illumina® GAII platform is based on the same technology used in ...
Catalog: 4454073
Size: 1 kit
Size: 1 kit
The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482335
Size: 8 reactions
Size: 8 reactions
The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482752
Size: 384 reactions
Size: 384 reactions
The Ion Xpress™ Plus Fragment Library Kit provides a rapid and flexible enzymatic-based library construction methodology for the Personal Genome Machine™ sequencing system workflow. Employing enhanced proprietary Ion Shear™ DNA fragmentation chemistry, this revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries, removing the need for a physical shearing device to save time and cost. With significantly higher yields and lower bias than other library construction techniques, superior coverage uniformity is attained with as little as 100...
Catalog: 4471269
Size: 10 reactions
Size: 10 reactions
The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482340
Size: 96 reactions
Size: 96 reactions
The Ion Xpress™ Barcode Adapters 1-16 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Ba...
Catalog: 4471250
Size: 1 kit
Size: 1 kit
The Ion Total RNA-Seq Kit for AB Library Builder™ System combines optimized reagents and protocols for automated preparation of representative cDNA libraries for strand-specific RNA sequencing of all types of RNA species on the Ion Personal Genome Machine (PGM™) and Proton™ sequencers. Either small RNA (such as microRNA) or whole transcriptome RNA samples can be prepared for next-generation sequencing. The Ion Total RNA-Seq Kit for AB Library Builder™ System contains the same performance advantages as the manual Ion Total RNA-Seq Kit v2 with the convenience of protocol automation. The gel size...
Catalog: 4482416
Size: 13 reactions
Size: 13 reactions
The Ion AmpliSeq™ RNA Apoptosis Panel is a screening tool designed against 267 genes involved in the cellular apoptosis pathway. Included are genes associated with death receptor-mediated apoptosis, c-Myc, and p53-mediated apoptosis. • Measure gene expression fold changes between samples • Amplicons are designed to be most inclusive for transcripts of the target gene • Each reaction requires only 500 pg of unfixed RNA or 5 ng of fixed (FFPE) RNA • Prepare amplicon libraries with the Ion AmpliSeq™ RNA Library Kit and sequence on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system This...
Catalog: 4482571
Size: 24 reactions
Size: 24 reactions
The Ion AmpliSeq™ Comprehensive Cancer Panel provides highly multiplexed target selection of genes implicated in cancer research. Encompassing over 50% of the Wellcome Trust Sanger Institute Cancer Gene Census, this is the most comprehensive cancer gene panel available. With all-exon coverage of 409 genes, the Ion AmpliSeq™ Comprehensive Cancer Panel delivers fast, FFPE-compatible, target selection for a broad survey of key genes for semiconductor sequencing. Download the Ion AmpliSeq™ Comprehensive Cancer Panel list of target genes (.csv) . The Ion AmpliSeq™ technology allows ultra-high multi...
Catalog: 4477685
Size: 8 reactions
Size: 8 reactions
The Ion AmpliSeq™ RNA Cancer Panel is a screening tool designed against 50 oncogenes/tumor suppressor genes found in the COSMIC database. This panel has been developed as an RNA complement to the DNA-focused Ion AmpliSeq™ Cancer Hotspot Panel v2. The panel is a single pool of primers representing 50 oncogenes and tumor suppressor genes with coverage of KRAS, BRAF, and EGFR genes. • Measure gene expression fold changes between samples • Amplicons are designed to be most inclusive for transcripts of the target gene • Each reaction requires only 500 pg of unfixed RNA or 5 ng of fixed (FFPE) RNA •...
Catalog: 4482572
Size: 24 reactions
Size: 24 reactions
The Ion OneTouch™ 200 Template Kit v2 contains template preparation reagents for automated and reproducible template preparation for 200 base-read libraries using the Ion OneTouch™ System . As an integral component of the semiconductor sequencing workflow, the Ion OneTouch™ 200 Template Kit v2 is designed to make high-quality templated Ion Sphere™ particles using the Ion OneTouch™ System to streamline the overall Ion sequencing workflow, yield significant time savings, increase productivity, and accelerate scientific discovery. The Ion OneTouch™ 200 Template Kit v2 provides you with: • Simple ...
Catalog: 4478316
Size: 10 reactions
Size: 10 reactions
The Ion Library Quantitation Kit is an easy-to-use, flexible solution for the accurate quantitation of library input for the Ion Torrent semiconductor sequencing workflow. This comprehensive kit provides a TaqMan® qPCR mix and library standard to detect and quantify femtomolar amounts of Ion Fragment Libraries. With universal cycling conditions that allow both fast and standard cycling, the Ion Library Quantitation kit supports most real-time platforms. This universal kit provides greater accuracy and specificity compared to non-probe-based methods, delivering simplicity and speed to the PGM™ ...
Catalog: 4468802
Size: 250 reactions
Size: 250 reactions
The TOPO® TA Cloning® Kits for Sequencing provide a highly efficient, 5 minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4-TOPO® TA vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction. These kits include everything necessary to clone and select recombinant vectors containing your PCR fragment of choice. Get More Sequence— Allows for more insert sequence and less vector seuquence when usin...
Catalog: K4530-20
Size: 20 reactions
Size: 20 reactions
The Ion PI™ Template OT2 200 Kit contains template preparation reagents for automated and reproducible template preparation using the Ion OneTouch™ 2 System for Ion Proton™ System semiconductor sequencing. As an integral component of the Ion Proton™ semiconductor sequencing workflow, the Ion PI™ Template OT2 200 Kit is designed to make high-quality templated Ion PI™ Ion Sphere™ particles using the Ion OneTouch™ 2 System to streamline the overall Ion sequencing workflow, yield significant time savings, increase productivity, and accelerate scientific discovery. The Ion PI™ Template OT2 Kit prov...
Catalog: 4482286
Size: 8 reactions
Size: 8 reactions
The Ion OneTouch™ 200 Template Kit v2 DL contains template preparation reagents for automated and reproducible template preparation for up to 300 base-read libraries using the Ion OneTouch™ System with the DL configuration. As an integral component of the semiconductor sequencing workflow, the Ion OneTouch™ 200 Template Kit v2 DL is designed to make high-quality templated Ion Sphere™ particles using the Ion OneTouch™ System to streamline the overall Ion sequencing workflow, yield significant time savings, increase productivity, and accelerate scientific discovery. The Ion OneTouch™ 200 Templat...
Catalog: 4480285
Size: 10 reactions
Size: 10 reactions
The Ion PGM™ Template OT2 400 Kit contains reagents for automated and reproducible template preparation for 400 base-read libraries using the Ion OneTouch™ 2 System. As an integral component of the semiconductor sequencing workflow, the Ion PGM™ Template OT2 400 Kit is designed to make high-quality, templated Ion Sphere™ particles using the Ion OneTouch™ 2 System to streamline the overall Ion sequencing workflow, yield significant time savings, increase productivity, and accelerate scientific discovery. The Ion PGM™ Template OT2 400 Kit provides you with: • Simple template preparation for 400 ...
Catalog: 4479878
Size: 10 reactions
Size: 10 reactions
Ion PGM™ Enrichment Beads are for use with Ion PGM™ template preparation kits for enrichment of template-positive Ion Sphere™Particles. The Ion PGM™ Enrichment Beads provide you with: • Simple enrichment for template-positive Ion Sphere™ Particles • Dynabeads® technology • Support for Ion PGM™ System workflow • Support for Ion OneTouch™ 200 Template Kit v2, Ion OneTouch™ Template Kit, Ion PGM™ 200 Xpress™ Template Kit, and Ion Xpress™ Template Kit The Ion PGM™ Enrichment Beads are streptavidin-coupled Dynabeads®, the gold standard for isolation due to their high binding affinity and uniformity...
Catalog: 4478525
Size: 10 reactions
Size: 10 reactions
Library Preparation Kits for Sequencing
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