Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing
The Ion Xpress™ Plus Fragment Library Kit provides a rapid and flexible enzymatic-based library construction methodology for the Personal Genome Machine™ sequencing system workflow. Employing enhanced proprietary Ion Shear™ DNA fragmentation chemistry, this revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries, removing the need for a physical shearing device to save time and cost. With significantly higher yields and lower bias than other library construction techniques, superior coverage uniformity is attained with as little as 100...
Catalog: 4471269
Size: 10 reactions
Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing
The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library dilutions for library normalization as required for any next generation sequencing workflow. The Ion Library Equalizer™ Kit helps reduce labor, reagent costs, and further simplifies the Ion semiconductor sequencing workflow for high sample-throughput or barcoded projects, as well as single or low sample number projects. The kit workflow consists of three simple steps: 1) Library amplification with Equalizer™ Primers 2) Library capture onto Equalizer™ Beads 3) He...
Catalog: 4482298
Size: 96 reactions
Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing
The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482335
Size: 8 reactions
Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing
The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482752
Size: 384 reactions
Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing
The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqMan® assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons can be found at www.lifetechnologies.com/confirmrna . Key Features: • Accurately measure gene expression fold changes from Ion AmpliSeq™ RNA panels • Start with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA • Ion Ampl...
Catalog: 4482340
Size: 96 reactions
Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing
The Ion Xpress™ Barcode Adapters 1-16 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Ba...
Catalog: 4471250
Size: 1 kit
Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing
The Ion AmpliSeq™ Comprehensive Cancer Panel provides highly multiplexed target selection of genes implicated in cancer research. Encompassing over 50% of the Wellcome Trust Sanger Institute Cancer Gene Census, this is the most comprehensive cancer gene panel available. With all-exon coverage of 409 genes, the Ion AmpliSeq™ Comprehensive Cancer Panel delivers fast, FFPE-compatible, target selection for a broad survey of key genes for semiconductor sequencing. Download the Ion AmpliSeq™ Comprehensive Cancer Panel list of target genes (.csv) . The Ion AmpliSeq™ technology allows ultra-high multi...
Catalog: 4477685
Size: 8 reactions
Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing
The Ion AmpliSeq™ RNA Apoptosis Panel is a screening tool designed against 267 genes involved in the cellular apoptosis pathway. Included are genes associated with death receptor-mediated apoptosis, c-Myc, and p53-mediated apoptosis. • Measure gene expression fold changes between samples • Amplicons are designed to be most inclusive for transcripts of the target gene • Each reaction requires only 500 pg of unfixed RNA or 5 ng of fixed (FFPE) RNA • Prepare amplicon libraries with the Ion AmpliSeq™ RNA Library Kit and sequence on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system This...
Catalog: 4482571
Size: 24 reactions
Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing
The Ion AmpliSeq™ RNA Cancer Panel is a screening tool designed against 50 oncogenes/tumor suppressor genes found in the COSMIC database. This panel has been developed as an RNA complement to the DNA-focused Ion AmpliSeq™ Cancer Hotspot Panel v2. The panel is a single pool of primers representing 50 oncogenes and tumor suppressor genes with coverage of KRAS, BRAF, and EGFR genes. • Measure gene expression fold changes between samples • Amplicons are designed to be most inclusive for transcripts of the target gene • Each reaction requires only 500 pg of unfixed RNA or 5 ng of fixed (FFPE) RNA •...
Catalog: 4482572
Size: 24 reactions
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing
The Pippin Prep™ System is a small tabletop instrument that automates the size selection and collection of DNA for PGM™ sequencer libraries. Simply enter the desired base-pair target range via the software interface and after a 1.5 hr run the system will yield the programmed DNA fractions with virtually no low molecular weight contamination. The tight fragment size distributions obtained provide a more uniform template for amplification. And the processing of samples is simplified through the use of disposable pre-cast agarose gel cassettes. Benefits of the Pippin Prep™ System • Time savings c...
Catalog: 4471271
Size: 1 each
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing
The personal-sized Ion Chip™ MiniFuge is designed and calibrated specifically for centrifugation of Ion PGM™/Ion Proton™ chips, enabling effective and efficient sample loading. The MiniFuge features a compact design with a small footprint. The cylindrical shape of the Ion Chip™ Minifuge allows it to accommodate both the Ion PGM™ and the larger Ion Proton™ chips. The unit quickly accelerates to maximum speed and stops quickly. • Tested and validated by Ion Torrent • Spins Ion PGM™ and Ion Proton™ chips, enabling effective and efficient sample loading • Small footprint, compact design The Ion Ch...
Catalog: 4479672
Size: 1 each
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing
The personal-sized Ion Chip™ MiniFuge is designed and calibrated specifically for centrifugation of Ion PGM™/Ion Proton™ chips, enabling effective and efficient sample loading. The MiniFuge features a compact design with a small footprint. The cylindrical shape of the Ion Chip™ Minifuge allows it to accommodate both the Ion PGM™ and the larger Ion Proton™ chips. The unit quickly accelerates to maximum speed and stops quickly. • Tested and validated by Ion Torrent • Spins Ion PGM™ and Ion Proton™ chips, enabling effective and efficient sample loading • Small footprint, compact design The Ion Ch...
Catalog: 4479673
Size: 1 each
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Sequencing & Genetic Analyzer Instrument Accessories
The Covaris® M220 microTUBE Prep Station enables the easy loading of sample into snap-cap and screw-cap microTUBEs. Each package contains 1 preparation station.
Catalog: 4482270
Size: 1 each
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Sequencing & Genetic Analyzer Instrument Accessories
The Covaris® M220 miniTUBE Prep Station enables the easy loading of sample into clear, blue, or red miniTUBEs. A tilted stand is included that has been designed to help recover the entire sample from the miniTUBE. Each package contains 1 preparation station.
Catalog: 4482267
Size: 1 each
Category: Products & Services > Controls, Standards & Ladders > Instrument Calibration Kits, Standards & Reagents > Sequencing Workflow Controls
The Ion Library Quantitation Kit is an easy-to-use, flexible solution for the accurate quantitation of library input for the Ion Torrent semiconductor sequencing workflow. This comprehensive kit provides a TaqMan® qPCR mix and library standard to detect and quantify femtomolar amounts of Ion Fragment Libraries. With universal cycling conditions that allow both fast and standard cycling, the Ion Library Quantitation kit supports most real-time platforms. This universal kit provides greater accuracy and specificity compared to non-probe-based methods, delivering simplicity and speed to the PGM™ ...
Catalog: 4468802
Size: 250 reactions
Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing
The Ion Total RNA-Seq Kit for AB Library Builder™ System combines optimized reagents and protocols for automated preparation of representative cDNA libraries for strand-specific RNA sequencing of all types of RNA species on the Ion Personal Genome Machine (PGM™) and Proton™ sequencers. Either small RNA (such as microRNA) or whole transcriptome RNA samples can be prepared for next-generation sequencing. The Ion Total RNA-Seq Kit for AB Library Builder™ System contains the same performance advantages as the manual Ion Total RNA-Seq Kit v2 with the convenience of protocol automation. The gel size...
Catalog: 4482416
Size: 13 reactions
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing
The Ion Chef™ System* is the next generation of workflow simplification products for the Ion Proton™ and Ion PGM™ systems. The Ion Chef™ System provides automated, high-throughput template preparation and reproducible chip loading for users at any experience level. With typically less than 15 minutes of hands-on time, an intuitive touch-screen interface, and significantly higher productivity than the Ion OneTouch™ 2 System, the Ion Chef™ System makes the Ion Torrent™ sequencing workflow accessible for every lab. The Ion Chef™ System: • Simplifies the workflow • Minimizes sources of user-introd...
Catalog: 4484177
Size: 1 system
Category: Products & Services > Instruments, Software, & Accessories > Instrument Accessories > Sequencing & Genetic Analyzer Instrument Accessories
The Ion Total RNA Library Protocol Card for AB Library Builder™System is required for running the Ion Total RNA-Seq Kit for AB for Library Builder™System . This protocol card contains the firmware needed to easily automate the creation of up to 13 whole transcriptome or small RNA (miRNA) libraries upstream of semiconductor sequencing on either the Ion PGM™ or Ion Proton™ platforms. The Ion Total RNA Library Protocol Card for AB Library Builder™System: • Enables whole transcriptome or small RNA (miRNA) library construction on the AB Library Builder™System • Eliminates the need for instrument pr...
Catalog: 4482563
Size: 1 card
Category: Products & Services > Instruments, Software, & Accessories > Instruments > Sample Prep Instruments for Sequencing
The Covaris® M220 Focused-ultrasonicator™ is designed for next generation sequencing applications that require high-quality DNA fragmentation for library preparation. The Covaris® M220 efficiently focuses the minimum amount of energy required directly into a sample, enabling precise control of the hydrodynamic shearing forces that fragment nucleic acids to the selected size. The process is isothermal, so fragmentation is unbiased, the sample is undamaged, and yields are high. The Covaris® focused-ultrasonic technology is considered a DNA shearing standard in many labs worldwide. The Covaris® M...
Catalog: 4482277
Size: 1 instrument