Expi293™ Expression Medium is a chemically-defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density cultures of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression. • Supports growth of suspension 293 cultures to densities of over 1.6 x 10 7 cells/mL, with >95% cell viability • Transfection-compatible serum-free suspension culture medium allowing transfection efficiencies of greater than 70% using ExpiFectamine...
Size: 1000 ml
Mouse Embryonic stem cells (ESCs) were isolated from mouse (Strain C57BL⁄6) embryos at Day E3.5. Before cryopreservation, the cells were tested for viability (>70% post-thaw viability), their ability to remain undifferentiated when cultured on mouse feeder cells as assayed by marker expression (Oct4, SSEA-1, Nanog) and their ability to differentiate into embryoid bodies and express markers indicative of three germ layers (Ectoderm: (+) for β3-tubulin and nestin, Mesoderm: (+) for smooth muscle actin, Endoderm: (+) for AFP). Cells were cryopreserved at passage 20 (P20). For optimal growth perfo...
Size: 1 ml
Lectin phytohemagglutinin-L (PHA-L) is strongly inhibited by N-acetylglucosamine β(1-2) mannopyranosyl residues.[ALTREF PN29369] This lectin has been shown to agglutinate leukocytes and induce mitogenic activity in T-lymphocytes[ALTREF PN29369,PN29368] and has also been used as an anterograde tracer for the study of neuronal pathways.[ALTREF PN20284]
Size: 1 mg
Knockout™ Serum Replacement (Knockout™ SR) is a defined, serum-free formulation used to support the growth of undifferentiated ES (Embryonic Stem Cells) and iPS (Induced Pluripotent Stem Cells) in culture. It directly replaces FBS in existing protocols. One of the most important parameters surrounding ES cell culture is the maintenance of ES cells in an undifferentiated state. Until now, FBS was the only option for nutritive supplementation of ES and iPS cell media. Unfortunately, FBS also contains factors which can promote differentiation of ES cells. Thus, each lot must be screened prior to ...
Size: 100 ml
This purified mouse anti- human EED antibody can be used in the validated application of immunoprecipitation (IP). Antibody characteristics include: • APPLICATIONS: EED Monoclonal Antibody, Recombinant Biotinylated Fab is validated for use in immunopreciptation. • HOST SPECIES AND ISOTYPE: This is a recombinant mouse monoclonal biotinylated Fab of human EED. • PRODUCT SIZE: 100 µg Specific for EED EED (embryonic ectoderm development) protein is a member of the polycomb-group (PcG) family that in humans is encoded by the EED gene. Two transcript variants encoding distinct isoforms have been ide...
Size: 100 µg
This is a purified mouse anti-human EED monoclonal antibody (mAb) that can be used in the validated application of immunoprecipitation (IP). Recombinant antibody characteristics include: • APPLICATIONS: This EED mouse recombinant mAb has been validated for use in IP. • HOST SPECIES AND ISOTYPE: This is a recombinant mouse monoclonal IgG1 antibody against human EED. • PRODUCT SIZE: 100 µg Specific for EED EED (embryonic ectoderm development) protein is a member of the polycomb-group (PcG) family that in humans is encoded by the EED gene. Two transcript variants encoding distinct isoforms have b...
Size: 100 µg
Gibco® PSC Neural Induction Medium is a serum-free medium that provides high efficiency neural induction of human pluripotent stems cells (PSCs) in only 7 days. Unlike existing methodologies, use of Gibco® PSC Neural Induction Medium does not require the intermediary step of embryoid body (EB) formation, which adds time, labor, and variability. Neural Stem Cells (NSCs) generated using Gibco® PSC Neural Induction Medium have high expression of NSC markers and can be further differentiated into other neural cell types. Note: The "prototype" status of this product means that the product is curren...
Size: 500 ml