ion indicators & ionophores
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+ . Fluo-3 has been used to image the spatial dynamics of Ca 2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt ...
Catalog: F-14200
Size: 500 µg
Size: 500 µg
The Calcium Calibration Buffer Kits are designed to help determine the dissociation constant (K d ) of fluorescent Ca 2+ indicators using a fluorometer or quantitative imaging system. The Kd of an indicator can be calculated from a plot generated by scanning the excitation or emission of the indicator in the presence of eleven different Ca 2+ concentrations. Calcium Calibration Kit #1 contains 50 mL of 10 mM K 2 EGTA and 50 mL of 10 mM CaEGTA. Both solutions contain 100 mM KCl and 30 mM MOPS, pH 7.2 and are prepared in deionized water. These stock solutions can be blended to make buffers havin...
Catalog: C-3008MP
Size: 1 kit
Size: 1 kit
Intracellular calibration of the fluorescence response of cytosolic pH indicators is typically performed using the K + /H + ionophore nigericin , which causes equilibration of intracellular and extracellular pH in the presence of a depolarizing concentration of extracellular K + .
Catalog: N-1495
Size: 10 mg
Size: 10 mg
Our highly purified lucigenin is a fluorescent Cl- indicator that has excitation/emission maxima of 368/505 nm and whose fluorescence is quenched in the presence of Cl-. The dye is useful for determining Cl- concentrations in liposomes, reconstituted membranes or in extracellular medium but is not useful for intracellular measurements.
Catalog: L6868
Size: 10 mg
Size: 10 mg
Fura-FF pentapotassium salt is a fura-2 derivative that has Ca 2+ sensitivity similar to mag-fura-2, but is relatively insensitive to Mg 2+ . This water-soluble salt form is useful for intracellular loading by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402).
Catalog: F14180
Size: 500 µg
Size: 500 µg
Carboxy-DCFDA can passively diffuse into cells and is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the fluorescent fluorophore, 5-(and-6)-carboxy-2',7' -dichlorofluorescein. With a pK a of 4.8, this dye can be used as an acidic pH sensor.
Catalog: C-369
Size: 100 mg
Size: 100 mg
To permit simultaneous long-term tracing of mixed-cell populations using different fluorescent colors, we have developed a probe whose applications should be similar to those of CFDA SE (commonly called CFSE). Cells labeled with our SNARF-1 carboxylic acid, acetate, succinimidyl ester have orange-red fluorescence that can be easily distinguished from that of cells loaded with green-fluorescent tracers such as CFDA SE.
Catalog: S22801
Size: 10 × 50 µg
Size: 10 × 50 µg
ion indicators & ionophores
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