DAF-FM is a reagent that is used to detect and quantify low concentrations of nitric oxide (NO). It is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and fluorometers. DAF-FM Specifications: • Ex/Em of DAF-FM: ~495/515 nm • Lyophilized product should be dissolved using DMSO and then added to an aqueous buffer to create a working solution • DAF-FM diacetate is cell permeant and passively diffuses across...
Size: 10 × 50 µg
Carboxy-DCFDA can passively diffuse into cells and is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the fluorescent fluorophore, 5-(and-6)-carboxy-2',7' -dichlorofluorescein. With a pK a of 4.8, this dye can be used as an acidic pH sensor.
Size: 100 mg
N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein’s N-1 formulation. N-2 Supplement is intended for use with Neurobasal® media. N-2 Supplement is recommended for growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). N-2 Supplement can be used as a substitute for Bottenstein’s N-1 formulation. N-2 Supplement is provided as a 100X concentrate. N-2 Supplement may be used with Neurobasal® media supplemented with growth factors such as bFGF and E...
Size: 5 ml
The Mouse IL-6 ELISA research use only kit is designed to detect and quantify the level of mouse IL-6 in serum, plasma, and supernatant using 96-well plates and a microplate reader. Easy to run Rapid protocol Convenient precoated, removable 8-well strips Easy-to-Run Sandwich ELISA The total assay incubation time is only 3.5 hours. A monoclonal capture antibody specific for Mouse IL-6 has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known Mouse IL-6 content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinyla...
Size: 192 tests
The IL-1RA ELISA Kit is designed to quantify protein levels of human interleukin-1 receptor antagonist (IL-1RA) in biological samples. IL-1RA is a cytokine which opposes the actions of IL-α and IL-1β by binding to IL-1 receptors.
Size: 96 tests
This is a purified rabbit anti-human IL9 monoclonal antibody (mAb) that can be used in validated applications such as western blotting (WB) and indirect ELISA. ABfinity™ antibody characteristics include: • APPLICATIONS: This IL9 Monoclonal Antibody, ABfinity™ Recombinant has been validated for use in western blotting and indirect ELISA. • HOST SPECIES AND ISOTYPE: This is a recombinant rabbit monoclonal IgG against human IL9. • CLONE ID OF MONOCLONAL (mAb): The monoclonal antibody clone is 2H14L3. • REACTIVITY: Along with human IL9, this monoclonal antibody is predicted to react with monkey. •...
Size: 100 µg