Dulbecco's Modified Eagle Medium : Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of Gibco® DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM / F-12 is modified as follows: With Without • L-glutamine • HEPES • Phenol Red
Size: 10 × 500 ml
Dulbecco's Modified Eagle Medium : Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of Gibco® DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM / F-12 is modified as follows: With • L-glutamine • HEPES • Phenol Red
Size: 500 ml
Advanced DMEM / F-12 (Dulbecco's Modified Eagle Medium / Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM / F-12, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology. i Cells successfully cultured in Advanced DMEM / F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat. This Advanced DMEM / F-12 is manufactured as follows: With Without • Glucose • L-glutamine • Non Essential Amino Acids • HEPES • Sodium pyruvate • Phenol Red
Size: 500 ml
Replacement Parts for Electrophoresis Apparatus
NuPAGE® Novex Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended Buffers: Run NuPAGE® Novex Tris-Acetate gels...
Size: 1 box
The Bioruptor® UCD-200 is a benchtop sonication device used to uniformly process up to 12 x 0.5 ml samples simultaneously in sealed tubes without aerosol formation. Consisting of a high-power ultrasound generating element located below a water bath, the Bioruptor® sonicates at a 20 kHz frequency, similar to a probe sonicator. The Bioruptor® comes with standard 0.5 ml accessories and the BioBox-200 for soundproofing. The Bioruptor® UCD-200 Provides: • Parallel processing of up to 12 DNA samples • Robust and reproducible results • Automated sample prep Get Reproducible DNA Shearing Easily and Au...
Size: 1 each
Size: 12 × 1 L
NuPAGE® Novex Bis-Tris Gels provide the best separation and resolution of small- to medium-sized proteins by utilizing a neutral pH environment which minimizes protein modifications. These high-performance gels have a one-year shelf life when stored at +4°C to 25°C, eliminating the waste of throwing out expired gels. Use NuPAGE® Novex Bis-Tris Gels for protein sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. The NuPAGE® System also provides the most efficient means for transferring proteins to membranes for subsequent analysis. Formulation: The NuPAGE...
Size: 1 box
Molecular Probes® ChromaTide® dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide® dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis. ChromaTide® Labeled Nucleotides Specifications: • Ex/Em of dye: Texas Red®-12-dUTP (595/615 nm) • Length...
Size: 25 µl
Novex® 8% Tris-Glycine Midi Gels (12+2 well) are polyacrylamide gels based on traditional Laemmli protein electrophoresis. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. Each box contains 10 gels. • Individually packaged gels for optimal experiment design • Compatible with most protein standards • Usable for native and denaturing protein assays Choose the Right Gel Format for Your Experiments Novex® Tris-Glycine gels come in a variety of fixed concentrations from 4% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, ...
Size: 1 box
The Molecular Probes® BioParticles® product line consists of a series of fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities. These fluorescent BioParticles® products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry. We offer E. coli (K-12 strain), S. aureus (Wood strain, without protein A) and zymosan ( S. cerevisiae ) BioParticles® conjugates covalently labeled with a variety of different fluorophores (special care has been taken to remove free dy...
Size: 10 mg
The CultureWell cell culture systems provide an integrated set-up for preparing cultured cells for staining and imaging. Each system uses medical-grade silicone gaskets preassembled with standard optical-quality coverslips in cell culture plates. The entire system is provided sterile and ready to use.
Size: 1 set
Applied Biosystems® MicroAmp® Caps Strips are designed to fit on MicroAmp® 0.2 mL Reaction Tubes, Reaction Tube Strips, and 96-Well Reaction Plates. They are available in 8-cap and 12-cap strips in clear and colored sets of red, orange, blue, and green. The MicroAmp® Optical 8-Cap Strip should be used for real-time PCR applications. Attach caps using the MicroAmp® Cap Installing Tool. Will not interfere with sample reading Provides tight seal to minimize evaporation Easy to apply and remove Minimizes Evaporation Use MicroAmp® Caps Strips to seal the tubes containing your precious samples. This...
Size: 200 strips of Assorted Colors
NuPAGE® Novex® 4-12% Bis-Tris Gels (1.0-mm thick, 17-well) are pre-cast polyacrylamide gels designed to give optimal separation of your small- to medium-sized proteins under denaturing conditions. Unlike traditional tris-glycine gels, NuPAGE® Novex® Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use the NuPAGE® Novex Bis-Tris Gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also use NuPAGE® gels to get optimal results for your day-to-day use. NuPAGE® Novex® Bis-Tris Gels come in two si...
Size: 10 gels⁄box
NATIVEPAGE 3-12% GELS, 10-WELL
Size: 1 each
Novex® Tris-Glycine Express Kits are a convenient and cost-effective way to obtain small quantities of pre-cast tris-glycine gels and pre-stained protein standard. Each kit includes: • 5 Novex® 4-20% Tris-Glycine Mini Gels, 1.0 mm, 12 well • 50 µl of SeeBlue® Pre-Stained Standard Novex® Tris-Glycine Mini Gels Novex® Tris-Glycine Mini Gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. • Individually packaged gels for optimal experiment design • Compatibl...
Size: 5 gels
For use with the Applied Biosystems® 7500 Real-Time PCR systems (with strip tube adaptor). For veterinary use only. Regulatory requirements vary by country; products may not be available in your geographic area. Biological hazard safety WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Safety equipment also may include items ...
Size: 80 strips
This is a purified mouse anti-human OLMF4 monoclonal antibody that can be used in the validated application of flow cytometry. This OLMF4 monoclonal antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant human OLFM4 and conjugated with FITC under optimum conditions. The unreacted FITC was removed. Applications: This OLMF4 monoclonal antibody has been validated for use in flow cytometry. Host Species and Isotype: This is a mouse monoclonal IgG1 against human OLFM4. Clone ID of Monoclonal Antibod...
Size: 100 tests
This is a purified mouse anti-human cadherin-12 monoclonal antibody that can be used in the validated application of flow cytometry. This cadherin-12 antibody was produced from a hybridoma, resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant human cadherin-12 and conjugated with PE under optimum conditions. The unreacted PE was removed. Applications: This cadherin-12 monoclonal antibody has been validated for use in flow cytometry. Host Species and Isotype: This is a mouse monoclonal IgG1 against human cadherin-12. Clone ID of Mo...
Size: 100 tests