Insulin to Supplement Cell Culture Media
Total Exosome Isolation (from cell culture media) enables fast and efficient enrichment of intact exosomes from cell culture media samples. • Maximize recovery of intact exosomes for any type of downstream application • Easily isolate exosomes using our simple and reliable protocol • Avoid time-consuming ultra-centrifugation • Maximize flexibility—can be scaled up or down depending on the sample size Isolation of exosomes can be a tedious, non-specific, and difficult process. Total Exosome Isolation (from cell culture media) enables simple and reliable concentration of intact exosomes from cel...
Catalog: 4478359
Size: 50 ml
Size: 50 ml
Exosome – Human CD63 Isolation/Detection enables the purification of CD63+ exosomes (also called extracellular vesicles and multi-vesicular bodies) from pre-enriched cell culture samples. These exosomes can then be detected using techniques such as flow cytometry, electron microscopy, or Western blotting. The exosomes must be pre-enriched before isolation. This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent. • Obtain highly pure CD63+ exosomes • "See" your sample during handling • Easily scalable protocol • Detec...
Catalog: 106-06D
Size: 3 ml
Size: 3 ml
The CultureWell cell culture systems provide an integrated set-up for preparing cultured cells for staining and imaging. Each system uses medical-grade silicone gaskets preassembled with standard optical-quality coverslips in cell culture plates. The entire system is provided sterile and ready to use.
Catalog: C-24760
Size: 1 set
Size: 1 set
The combination of the antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective action against gram-positive and gram-negative bacteria, respectively. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus . Streptomycin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibit...
Catalog: 15070-063
Size: 100 ml
Size: 100 ml
Fungizone® antimycotic is a formulation of Amphotericin B with sodium deoxycholate as an emulsifying agent to maintain the suspension. Amphotericin B is used to prevent the growth of fungi by causing an increase in fungal plasma membrane permeability. Gibco® Fungizone® antimycotic is used for the prevention of yeast and multicellular fungi contamination of cell cultures. Gibco® Fungizone contains 250 µg of amphotericin B and 205 µg of sodium deoxycholate per ml in distilled water. The recommended working concentration ranges from 0.25 to 2.50 µg/ml. Life Technologies offers a variety of antibi...
Catalog: 15290-018
Size: 20 ml
Size: 20 ml
Gibco® Insulin, Transferrin, Selenium, Sodium Pyruvate Solution (ITS -A) is used as a basal medium supplement in order to reduce the amount of Fetal Bovine Serum (FBS) used to culture the cells. Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G is now routinely used with other basal media to support a wide variety of cell types in the presence of 2-4% FBS. Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and it may also help to reduce toxic levels of oxyge...
Catalog: 51300-044
Size: 10 ml
Size: 10 ml
N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein’s N-1 formulation. N-2 Supplement is intended for use with Neurobasal® media. N-2 Supplement is recommended for growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). N-2 Supplement can be used as a substitute for Bottenstein’s N-1 formulation. N-2 Supplement is provided as a 100X concentrate. N-2 Supplement may be used with Neurobasal® media supplemented with growth factors such as bFGF and E...
Catalog: 17502-048
Size: 5 ml
Size: 5 ml
Gibco® HAT Supplement is a liquid mixture of sodium hypoxanthine (5 mM), aminopterin (20 µM) and thymidine (0.8 mM). HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT–myeloma cells. Hypoxanthine and thymidine supply preformed purines and pyrimidines for DNA synthesis by hybridomas via the salvage pathway that utilizes HGPRT- contributed by the fused spleen cell. Aminopterin, a folic acid antagonist, inhibits the de novo nucleoside biosynthesis pathway. Product Use For Research Use Only. Not for use in diagnostic procedures. Dual site cGMP Manufac...
Catalog: 21060-017
Size: 100 ml
Size: 100 ml
A sterile set of solutions intended for use with EpiLife® medium or Medium 154 to culture human epidermal keratinocytes. The HKGS Kit provides, in separate vials, all the components of complete HKGS (S-001-5). A vial of gentamycin/amphotericin solution (GA) is also included. Use of GA is optional.
Catalog: S-001-K
Size: 1 kit
Size: 1 kit
A defined, sterile, concentrated (100X) solution intended for use with EpiLife® medium to culture human epidermal keratinocytes. EDGS is not intended for use with Medium 154. EDGS contains all of the growth factors and hormones necessary for the growth of human epidermal keratinocytes. Each bottle contains the correct amount of supplement for a 500 ml bottle of EpiLife® medium. EDGS contains: • purified bovine serum albumin • purified bovine transferrin • hydrocortisone • recombinant human insulin-like growth factor type-1 (rhIGF-1) • prostaglandin E2 (PGE2) • recombinant human epidermal growt...
Catalog: S-012-5
Size: 5 ml
Size: 5 ml
Custom Media: 2-MERCAPTOETHANOL
Catalog: A13558SA
Size: 1 each
Size: 1 each
Custom Media: MAGNESIUM CHLORIDE ANHYDROUS
Catalog: A13735IN
Size: 1 each
Size: 1 each
Custom Media: N2B27 CUSTOM MEDIA
Catalog: ME100137L1
Size: 1 each
Size: 1 each
Gibco® Hepatocyte Thaw Media is a ready-to-use media specifically designed for use with thawed cryopreserved hepatocytes to enhance their viability and overall quality. This media is provided in a 50 mL conical tube for ease of use. Features of Hepatocyte Thaw Media: • Easy to use—simply warm up to 37°C and add thawed hepatocytes • Recover cryopreserved hepatocytes with a single centrifugation • Designed for use with Gibco® human and animal cryopreserved hepatocytes for optimal results • Provides high quality hepatocytes suitable for many in vitro assays, including induction, metabolic stabili...
Catalog: CM7500
Size: 50 ml
Size: 50 ml
StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell su...
Catalog: A11105-01
Size: 100 ml
Size: 100 ml
Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Phosphate Buffered Saline (PBS). Gibco® Cell Dissociation Buffer is suitable for gentle dissociation of mammalian cells for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines such as HeLa and NIH 3T3, but is not recommended for routine passage of strongly adherent cells. The dis...
Catalog: 13151-014
Size: 100 ml
Size: 100 ml
Human Endothelial-SFM is a basal serum-free growth medium developed to support the isolation and expansion of human umbilical venous and arterial endothelial cells (1–4). Medium requires supplementation with bFGF (20 ng/ml), EGF (10 ng/ml), and human plasma fibronectin (10 µg/ml). Contains L-glutamine.
Catalog: 11111-044
Size: 500 ml
Size: 500 ml
Gibco® CD Hybridoma Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features: • Ability to support suspension and stationary hybridomas and myelomas • Formulated without L-glutamine for stability • Animal origin-free, protein-free and chemically defined formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® CD Hybridoma Medi...
Catalog: 11279-023
Size: 1,000 ml
Size: 1,000 ml
Defined Keratinocyte-SFM is a serum-free medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers (1,2). The growth-promoting activity of BPE has been replaced by the inclusion of growth promoting agents which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration ( Note: This formulation contains s...
Catalog: 10744-019
Size: 500 ml
Size: 500 ml
StemPro®-34 SFM is a serum-free medium specifically formulated to support the development of human hematopoietic cells in culture (1). StemPro®-34 SFM can be used to study hematopoietic cells from bone marrow, peripheral blood, or neonatal cord blood. StemPro®-34 SFM is performance-tested on normal human bone marrow. It is manufactured with components of human, recombinant or synthetic origin. StemPro®-34 SFM consists of a basal liquid medium and frozen supplement (StemPro®-Nutrient Supplement) which is added at the time of use.
Catalog: 10639-011
Size: 500 ml
Size: 500 ml
Insulin to Supplement Cell Culture Media
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