Identifiler® Direct Kit

AmpFLSTR® Yfiler® Direct Kit

Category: Products & Services > Kits & Assays > Forensic Testing Kits > Forensic DNA Identification Kits

The AmpFLSTR® Yfiler® Direct Kit enables forensic laboratories to perform high-quality Y-STR analysis of single-source reference samples using direct PCR amplification. This kit amplifies the same 17 Y-STR loci as the AmpFLSTR® Yfiler® Kit and is designed to generate accurate results with faster thermocycling conditions than the Yfiler® Kit. Blood or buccal samples on treated paper (FTA® Card or NUCLEIC-CARD™ System ) can be punched into PCR plates or tubes and taken directly to PCR amplification, without reduction in data quality. Samples collected with the Buccal DNA Collector™ and other unt...

Catalog: 4477555
Size: 250 tests

Champion™ pET100 Directional TOPO® Expression Kit with BL21 Star™ (DE3) One Shot® Chemically Competent E. coli

Category: Products & Services > Kits & Assays > Protein Expression Kits > Bacterial Expression Kits

The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...

Catalog: K100-01
Size: 20 reactions

Champion™ pET101 Directional TOPO® Expression Kit with BL21 Star™ (DE3) One Shot® Chemically Competent E. coli

Category: Products & Services > Kits & Assays > Protein Expression Kits > Bacterial Expression Kits

The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...

Catalog: K101-01
Size: 20 reactions

AmpFLSTR® Identifiler® Plus PCR Amplification Kit User Guide

Category: Products & Services > Literature > Manuals > User Guides, Manuals & Bulletins

This is the User Guide Manual for the AmpFLSTR® Identifiler® Plus PCR Amplification Kit. Note: For Forensic or Paternity Use Only.

Catalog: 4440211
Size: 1 manual

pBAD202 Directional TOPO® Expressions Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Bacterial Expression Vectors

Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...

Catalog: K4202-01
Size: 20 reactions

pcDNA™3.1 Directional TOPO® Expression Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

The pcDNA™3.1 Directional TOPO® Expression Kit uses linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO® for five-minute directional cloning and subsequent high-level expression. Directional Cloning technology facilitates expression experiments because: A proofreading enzyme is used for fewer errors in cloned genes Greater than 90% of the clones are in the correct orientation for expression, reducing time spent colony screening for clone orientation In addition, pcDNA3.1D/V5-His-TOPO® provides strong expression levels from the CMV promoter and the option of a C-terminal V5-His fusion t...

Catalog: K4900-40
Size: 40 reactions

pLenti6/V5 Directional TOPO® Cloning Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

The pLenti6⁄V5 Directional TOPO® Cloning Kit contains the TOPO®-adapted ViraPower™ lentiviral expression vector, pLenti6⁄V5-D-TOPO® for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving high-level, constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells. Advantages • High efficiency and rapid cloning • Constitutive gene expression in dividing and non-dividing mammalian cells in vitro or in vivo • Produces replication-incompet...

Catalog: K4955-10
Size: 20 reactions

ViraPower™ Lentiviral Directional TOPO® Expression Kit

Category: Products & Services > DNA Vectors, Clones, Purified Nucleic Acids & Libraries > DNA Vectors > Mammalian Expression Vectors

The ViraPower™ Lentiviral Directional TOPO® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. It combines Invitrogen’s ViraPower™ Lentiviral and Directional TOPO® technologies to facilitate quick PCR-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6⁄V5-D-TOPO® vector has the CMV promoter for driving constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells. Advantages • Lentivirus...

Catalog: K4950-00
Size: 1 kit