Hydrazide Biotin
Biotin-14-dATP is a patented dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker. The long, 14-atom spacer arm allows accessibility to streptavidin for sensitive detection of probe-target hybrids in in situ and filter-based hybridizations. Biotin-14-dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP, dGTP, and dTTP (1). Biotin-14-dATP can be added to the 3´ end of DNA using terminal deoxynucleotidyl transferase (2). The amount supplied is sufficient for labeling up to 50 µg of DNA by nick translation. Performance and ...
Catalog: 19524-016
Size: 50 nmol
Size: 50 nmol
CaptAvidin™ biotin-binding protein contains a nitrated tyrosine in its biotin-binding site. This chemical modification permits dissociation of the avidin-biotin complex under milder conditions than the traditional avidin-biotin complex. At pH 4.0, CaptAvidin™ biotin-binding protein binds biotin tightly with a K a of ~10 9 M -1 . This association can then be reversed at pH 10, allowing complete dissociation of the avidin-biotin complex. This is in contrast to the harsh conditions (8 M guanidine hydrochloride, pH 1.5) needed to dissociate the avidin-biotin complex.
Catalog: C-21385
Size: 1 mg
Size: 1 mg
Our NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding.
Catalog: A-2666
Size: 5 mg
Size: 5 mg
Goat whole molecule anti-human immunoglobulin Format/Formulation: Biotin Conjugate
Catalog: AHI0309
Size: 0.7 mg⁄ml
Size: 0.7 mg⁄ml
Clone/PAD: Z021 Immunogen: Synthetic biotin Specificity: Binds strongly to biotinylated proteins. Reactivity: Biotinylated proteins Applications: ELISA, Cell and tissue staining
Catalog: 03-3720
Size: 1 ml
Size: 1 ml
F4/80, Rat Anti-Mouse, (Biotin); Biotinylated Monoclonal antibody specific to Mouse F4/80. This antibody is validated for use in Flow Cytometry. Anti-F4/80 recognizes the expressed product of the Emr1 gene.
Catalog: MF48015
Size: 1 ml
Size: 1 ml
The Alexa Fluor 488 hydrazide is a low molecular weight, cell membrane—impermeant, aldehyde-fixable molecule that can be used as a cell tracer. This green-fluorescent polar dye can be loaded into cells by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402).
Catalog: A-10436
Size: 1 mg
Size: 1 mg
This kit is used to block endogenous biotin or biotin-binding activity in tissue sections. The kit contains 18 mL each of Avidin and Biotin solutions.
Catalog: 00-4303
Size: 1 kit
Size: 1 kit
Our horseradish peroxidase (HRP) conjugate of NeutrAvidin® biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding.
Catalog: A-2664
Size: 1 mg
Size: 1 mg
Our streptavidin, Marina Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ~365/460 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.
Catalog: S-11221
Size: 1 mg
Size: 1 mg
The thiol-reactive Alexa Fluor 546 C 5 maleimide and its conjugates are perfect for applications that require orange-fluorescent probes. Spectrally similar to the Cy3 dye, the Alexa Fluor 546 dye is readily excited by the 546 nm line of the mercury-arc lamp and the 543 nm line of the He-Ne laser.
Catalog: A-10258
Size: 1 mg
Size: 1 mg
The amine-reactive Lissamine™ rhodamine B sulfonyl chloride can be used to create red-fluorescent bioconjugates with excitation/emission maxima ~568/583 nm.
Catalog: L-20
Size: 1 g
Size: 1 g
Our 4-acetamido-4'-maleimidylstilbene-2,2'- disulfonic acid is a thiol-reactive reagent that is water soluble, with high polarity and membrane impermeability. This polysulfonated dye is useful for determining whether thiol-containing proteins and polypeptide chains are exposed at the extracellular or cytoplasmic membrane surface.
Catalog: A-485
Size: 25 mg
Size: 25 mg
Reactivity: FITC-SA will react with biotin.
Catalog: 43-4311
Size: 1 mg
Size: 1 mg
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Catalog: F-150
Size: 25 mg
Size: 25 mg
DTNB or Elman's reagent can be used to quantitate thiols in proteins, cells and plasma by absorption measurements. It readily forms a mixed disulfide with thiols, liberating the chromophore 5-merapto-2-nitrobenzoic acid (absorption maximum 410 nm). Only protein thiols that are accessible to this water-soluble reagent are modified.
Catalog: D-8451
Size: 10 g
Size: 10 g
The pcDNA™6 BioEase™ Gateway® Biotinylation System provides an easy, efficient method for expressing, purifying, and detecting biotinylated recombinant proteins. The pcDNA™6/BioEase™-DEST vector (Figure 1) incorporates a 72 amino acid sequence from K. pneumoniae that directs in vivo biotinylation of a specific lysine residue. This K. pneumoniae tag is recognized and efficiently biotinylated in vivo by a native mammalian system similar to the enzyme Biotin Protein Ligase (BPL) in E. coli (1). Proteins produced from the pcDNA™6/BioEase™-DEST vector are expressed as fusion proteins to this sequen...
Catalog: SA100-02
Size: 50 µl
Size: 50 µl
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
Catalog: C-6164
Size: 10 mg
Size: 10 mg
Reactivity: HRP-Avidin reacts with biotin. Applications: Immunohistochemistry, ELISA
Catalog: 43-4423
Size: 5 mg
Size: 5 mg
Streptavidin-Horseradish Peroxidase (HRP) conjugate
Catalog: SA100-01
Size: 50 µl
Size: 50 µl
Hydrazide Biotin
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