High Resolution Melt Kits
Applied Biosystems® MeltDoctor™ HRM Master Mix contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets. High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. Unlike some competitive mixes, the MeltDoctor™ HRM Master Mix does not require additional mixing prior to use, and was developed and optimized solely for HRM applications. • Low background fluorescence • High brightness in the presence of double-stranded DNA • Minimal temperature shif...
Catalog: 4409535
Size: 50 ml
Size: 50 ml
The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of input total RNA in a single 20 µL reaction to single stranded cDNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Includes sufficient reagents for 1000 x 20 µL reactions. • Linear target amplification for real-time PCR • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost • 10-fold greater dynamic range than other kits cDNA from Total RNA The High Capacity cDNA Reverse Transcription Kit (for...
Catalog: 4374967
Size: 1000 reactions
Size: 1000 reactions
The High Capacity RNA-to-cDNA™ Kit is a streamlined reverse transcription kit designed for optimum performance with the TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix, and other PCR enzymes. Features of the High Capacity RNA-to-cDNA™ Kit: • Fast —Short reaction time (typically 30–60 min) • Convenient —Easy workflow with few pipetting steps (2 tubes) • Reliable —Reliable reverse transcription of both abundant and limited targets • Flexible —Optimized 2-step protocol enabling multiple PCR reactions from a single reverse transcription reaction Streamline Your cDNA Synthesis ...
Catalog: 4387406
Size: 50 reactions
Size: 50 reactions
Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA; up to 39 µg of RNA per reaction • Extra convenience; cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped...
Catalog: AM1344
Size: 25 reactions
Size: 25 reactions
The RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensur...
Catalog: R33700
Size: 1 kit
Size: 1 kit
The NA-Fluor™ Influenza Neuraminidase Assay Kit provides validated reagents and a standardized protocol for conducting neuraminidase enzyme assays, including neuraminidase inhibitor (NI) susceptibility screening using a fluorescent MUNANA substrate. Key product features: • Efficient design—neuraminidase assay in one complete kit • Optimized formulations—assay reagents have been optimized for maximum performance based on NISN protocols • Robust application—functional detection of NI-resistant virus in mixed viral populations • Easy of use & flexible screening—stable fluorescent signal enables b...
Catalog: 4457091
Size: 960 assays
Size: 960 assays
The Vybrant Apoptosis Assay Kit #4 detects apoptosis on the basis of changes that occur in the permeability of cell membranes. This kit contains ready-to-use solutions of both YO-PRO-1 and propidium iodide nucleic acid stains. YO-PRO-1 stain selectively passes through the plasma membranes of apoptotic cells and labels them with moderate green fluorescence. Necrotic cells are stained red-fluorescent with propidium iodide.
Catalog: V13243
Size: 1 kit
Size: 1 kit
The Ion Xpress™ Barcode Adapters 1-16 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Ba...
Catalog: 4471250
Size: 1 kit
Size: 1 kit
The LIVE/DEAD® Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. Calcein violet AM and aqua-fluorescent reactive dye are optimal dyes for this application; both dyes utilize the violet laser allowing other laser lines to be used for conventional fluorochromes.
Catalog: L34958
Size: 1 kit
Size: 1 kit
The Mouse IL-6 ELISA research use only kit is designed to detect and quantify the level of mouse IL-6 in serum, plasma, and supernatant using 96-well plates and a microplate reader. Easy to run Rapid protocol Convenient precoated, removable 8-well strips Easy-to-Run Sandwich ELISA The total assay incubation time is only 3.5 hours. A monoclonal capture antibody specific for Mouse IL-6 has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known Mouse IL-6 content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinyla...
Catalog: KMC0062
Size: 192 tests
Size: 192 tests
This Ambion® kit is for the purification of bacterial mRNA by removing rRNA from total RNA. Sufficient reagents for 20 mRNA purifications, each from 10 µg of total RNA are provided. Features of the MICROB Express ™ Kit: • Dramatically increase the sensitivity of array analyses and other procedures • Removes >95% of 16S and 23S rRNA from bacterial RNA • Simple procedure takes less than 2 hours • Works with many Gram positive and Gram negative bacteria Rapid Purification of Bacterial mRNA is Now Possible Isolating mRNA from bacteria has been virtually impossible, until now. The MICROB Express ™ ...
Catalog: AM1905
Size: 20 reactions
Size: 20 reactions
The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates. • Improve the quality of your results for a wide range of sequencing applications. • Optimized for long read lengths. • Better dye mobility characteristics • Improved performance reading through GT rich regions. • Get longer, higher-quality reads with more uniform peak heights and optimal signal balance. • Enhance your productivity and reduce costs. Get More Uniform Peak Heights, Improved Accuracy...
Catalog: 4337458
Size: 25000 reactions
Size: 25000 reactions
Applied Biosystems® Protein Thermal Shift™ kits allow users to employ a dye that binds to exposed hydrophobic residues to monitor the thermal stability of proteins using a real-time PCR instrument in order to identify buffer conditions that stabilize the protein of interest or screen libraries of ligands for compounds that bind the protein of interest. Save Time and Money with Your Sample Screening This solution significantly reduces the time and cost associated with screening samples relative to currently available methods. Protein Thermal Shift™ products that are run on our real-time PCR sys...
Catalog: 4462263
Size: 2000 reactions
Size: 2000 reactions
The DS-30 Matrix Standard kit contains 4 oligonucleotides respectively labeled with 6-FAM™, HEX™, NED™, and ROX™, pre-pooled in a single tube. This Matrix Standard Set is used to generate the "multicomponent matrix" required when analyzing fluorescent dye-labeled DNA fragments on the Applied Biosystems® 3100, 3130, and 3730 Series Systems. The Data Collection Software for these instruments uses the multicomponent matrix to automatically analyze the four different colored fluorescent dye-labeled samples in a single capillary. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4345827
Size: 8 runs
Size: 8 runs
The PureLink® HiPure Plasmid Purification Systems are designed to isolate transfection-grade plasmid DNA from E. coli in less than 2 hours. The Maxiprep Kit protocol will typically yield 500–850 µg plasmid DNA from 100–200 ml bacterial culture, at a purity that is comparable to that achieved with two passes through cesium chloride gradients. Advantages of the PureLink® HiPure Plasmid Maxiprep Kit include: • Labor- and time-saving —No need for extra steps to remove endotoxin and other contaminants • High purity, low endotoxin product —pure enough for mammalian cell transfection • Versatility —p...
Catalog: K2100-07
Size: 25 preps
Size: 25 preps
Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. • High yields of capped RNA—up to 39 µg of RNA per reaction • Extra convenience—cap analog-nucleotide mix provided • Simplified reaction format • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped R...
Catalog: AM1348
Size: 25 reactions
Size: 25 reactions
The Alexa Fluor 488 Oligonucleotide Amine Labeling Kit provides a simple method for labeling amine-modified oligonucleotides with our bright, photostable, green-fluorescent Alexa Fluor 488 dye (which is spectrally similar to fluorescein). Oligonucleotides labeled with this kit can be used as primers and as probes in hybridization experiments but should not be used for automated DNA sequencing since the Alexa Fluor dye in the kit is supplied as mixed isomers. The kits have been optimized for labeling reactions with 50 µg of a 5'-amine—modified oligonucleotide, 18 to 24 bases in length.
Catalog: A-20191
Size: 1 kit
Size: 1 kit
The Ion Sphere™ Quality Control Kit furnishes a complete set of reagents for performing templated bead quality control using the Qubit® 2.0 Fluorometer. This comprehensive kit contains calibration controls, buffers, and a probe mix for quality assessment of pre-enriched and post-enriched template-positive Ion Spheres. The powerful combination of the Ion Sphere™ Quality Control Kit and the Qubit® 2.0 Fluorometer gives you greater experimental control and significant time and cost savings. By providing the confidence to generate high quality sequencing data with every run, this quality control p...
Catalog: 4468656
Size: 1 kit
Size: 1 kit
Th e GeneBLAzer® Jurkat Control Kit contains constitutively expressing (CMV- bla ) and wildtype Jurkat cells to provide positive and negative controls, respectively, for evaluating beta-lactamase activity (Figure 1).
Catalog: K1206
Size: 1 kit
Size: 1 kit
FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit , which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. FISH Tag™ Kit Specificatio...
Catalog: F32947
Size: 1 kit
Size: 1 kit
High Resolution Melt Kits
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