high-content imaging

HCS LipidTOX™ Red Neutral Lipid Stain, for cellular imaging

Category: Products & Services > Kits & Assays > Cell Analysis Kits > HCS Detection Kits

The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid sta...

Catalog: H34476
Size: 1 each

HCS LipidTOX™ Green Neutral Lipid Stain, for cellular imaging

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Green neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid s...

Catalog: H34475
Size: 1 each

Fluo-4, Pentapotassium Salt, cell impermeant

Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+ . Fluo-3 has been used to image the spatial dynamics of Ca 2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt ...

Catalog: F-14200
Size: 500 µg

CellMask™ Deep Red Plasma membrane Stain

Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains

The CellMask™ Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. These stains may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for standard fluorescence microscopy. The stain survives fixation, but not permeabilization, so it is not suitable for experiments that also involve probing internal targets via antibodies.

Catalog: C10046
Size: 100 µl

HCS CellMask™ Red Stain

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

HCS CellMask™ Red stain (H32712) replaces the HCS CellMask™ Red cytoplasmic⁄nuclear stain (H32711) as a cell delineation tool for HCS platforms. HCS CellMask™ stains label the entire cell (cytoplasm and nucleus) and provide an accurate backdrop against which the features of interest can be assessed. HCS CellMask™ stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. If only nuclear staining is desired, HCS NuclearMask™ stains can be used for measuring DNA content in ...

Catalog: H32712
Size: 1 set