High Content Analysis
The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid sta...
Catalog: H34476
Size: 1 each
Size: 1 each
HCS CellMask™ Red stain (H32712) replaces the HCS CellMask™ Red cytoplasmic⁄nuclear stain (H32711) as a cell delineation tool for HCS platforms. HCS CellMask™ stains label the entire cell (cytoplasm and nucleus) and provide an accurate backdrop against which the features of interest can be assessed. HCS CellMask™ stains can be applied to cells immediately after fixation or in the last step of multiplexing protocols, and they are compatible with detergent-based cell permeabilization protocols. If only nuclear staining is desired, HCS NuclearMask™ stains can be used for measuring DNA content in ...
Catalog: H32712
Size: 1 set
Size: 1 set
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+ . Fluo-3 has been used to image the spatial dynamics of Ca 2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt ...
Catalog: F-14200
Size: 500 µg
Size: 500 µg
The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX™ Green neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid s...
Catalog: H34475
Size: 1 each
Size: 1 each
The CellMask™ Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. These stains may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for standard fluorescence microscopy. The stain survives fixation, but not permeabilization, so it is not suitable for experiments that also involve probing internal targets via antibodies.
Catalog: C10046
Size: 100 µl
Size: 100 µl
The BRAF Mutation Analysis Reagents employ a simple fragment analysis workflow to detect 1% mutant to wild type genomic DNA across 3 common and rare mutations in the BRAF gene region (see Table 1). The BRAF set provides sufficient reagent for analysis of 30 samples. Advantages of the BRAF Mutation Analysis Reagents include: • Simple Analysis—unambiguous, easy-to-interpret results • Thorough Coverage—detects 3 mutations in the BRAF gene • Sensitive—detects mutations at frequencies as low as 1 to 5% against a wild type genomic DNA background • Efficient—interrogate multiple loci from the same sa...
Catalog: 4452136
Size: 1 kit
Size: 1 kit
This product can only be used to upgrade Sequencing Analysis Software v5.3.1 installed on standalone computers, computers separate to the instrument data collection system. Please talk with your representative to upgrade the data collection system and secondary analysis software running on the data collection computer. Sequencing Analysis Software enables you to basecall, trim, display, edit, and print data from our entire line of capillary DNA sequencing instruments for data analysis and quality control. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4310991
Size: 1 CD
Size: 1 CD
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES
Catalog: 11960-069
Size: 10 × 500 ml
Size: 10 × 500 ml
The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of input total RNA in a single 20 µL reaction to single stranded cDNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Includes sufficient reagents for 1000 x 20 µL reactions. • Linear target amplification for real-time PCR • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost • 10-fold greater dynamic range than other kits cDNA from Total RNA The High Capacity cDNA Reverse Transcription Kit (for...
Catalog: 4374967
Size: 1000 reactions
Size: 1000 reactions
Platinum® Taq DNA Polymerase High Fidelity is ideal for amplification of DNA fragments where high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´a5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase (Table 1). PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology. With Platinum® Taq High Fidelity you'll get: Greater than six times higher fidelity than Taq DNA poly-merase Amplification of fragments up to 15 kb (Figure 1) Room temperature reaction...
Catalog: 11304-102
Size: 5000 reactions
Size: 5000 reactions
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • Sodium Pyruvate • L-glutamine • HEPES • Phenol Red
Catalog: 11965-126
Size: 6 × 1000 ml
Size: 6 × 1000 ml
High-Yield Lyse is a premixed, fixative-free erythrocyte lysing solution that can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with Invitrogen's fluorochrome-conjugated monoclonal antibodies.
Catalog: HYL-250
Size: 500 ml
Size: 500 ml
AccuPrime™ Taq DNA Polymerase High Fidelity amplifies nucleic acid templates using antibody-mediated hot-start, a blend of Taq DNA Polymerase and proofreading enzyme, and AccuPrime™ accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases. With AccuPrime™ Taq DNA Polymerase High Fidelity you'll get: The highest specificity and yield for the most robust PCR amplification 9-fold higher fidelity than Taq DNA polymerase alone Mimimal optimization steps, even with non-optimized primer sets Efficient amplification of targets over a broad size range up...
Catalog: 12346-094
Size: 1000 reactions
Size: 1000 reactions
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES • L-methio...
Catalog: 21013-024
Size: 500 ml
Size: 500 ml
Our highly purified lucigenin is a fluorescent Cl- indicator that has excitation/emission maxima of 368/505 nm and whose fluorescence is quenched in the presence of Cl-. The dye is useful for determining Cl- concentrations in liposomes, reconstituted membranes or in extracellular medium but is not useful for intracellular measurements.
Catalog: L6868
Size: 10 mg
Size: 10 mg
This highly cross-adsorbed Alexa Fluor® 594 Goat Anti-Rabbit IgG (H+L) is labeled with our superior red-fluorescent Alexa Fluor 594 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. To minimize cross-reactivity, it has been adsorbed against bovine IgG, goat IgG, mouse IgG, rat IgG and human IgG.
Catalog: A-11037
Size: 0.5 ml
Size: 0.5 ml
This highly cross-adsorbed Alexa Fluor® 488 Goat Anti-Mouse IgG (H+L) is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from mouse. To minimize cross-reactivity, it has been adsorbed against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG and human serum.
Catalog: A-11029
Size: 0.5 ml
Size: 0.5 ml
The High Capacity RNA-to-cDNA™ Kit is a streamlined reverse transcription kit designed for optimum performance with the TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix, and other PCR enzymes. Features of the High Capacity RNA-to-cDNA™ Kit: • Fast —Short reaction time (typically 30–60 min) • Convenient —Easy workflow with few pipetting steps (2 tubes) • Reliable —Reliable reverse transcription of both abundant and limited targets • Flexible —Optimized 2-step protocol enabling multiple PCR reactions from a single reverse transcription reaction Streamline Your cDNA Synthesis ...
Catalog: 4387406
Size: 50 reactions
Size: 50 reactions
This highly cross-adsorbed Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L) is labeled with our bright, photostable, green-fluorescent Alexa Fluor 488 dye and is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. To minimize cross-reactivity, it has been adsorbed against bovine IgG, goat IgG, mouse IgG, rat IgG and human IgG.
Catalog: A-11034
Size: 0.5 ml
Size: 0.5 ml
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES
Catalog: 31053-028
Size: 500 ml
Size: 500 ml
High Content Analysis
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