hanks' balanced salt solution solutions

PBS, pH 7.4

Category: Products & Services > Cell Culture & Transfection Reagents > Balanced Salt Solutions > Phosphate-Buffered Salines

Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Life Technologies offers a variety of Gibco® PBS formulations for a range of cell culture applications. Find the right formulation using the media selector tool . This PBS is manufactured as follows: Without • Calcium • Magnesium • Phenol Red
Catalog: 10010-023
Size: 500 ml

PBS, pH 7.2

Category: Products & Services > Cell Culture & Transfection Reagents > Balanced Salt Solutions > Phosphate-Buffered Salines

Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Life Technologies offers a variety of Gibco® PBS formulations for a range of cell culture applications. Find the right formulation using the media selector tool . This PBS is manufactured as follows: Without • Calcium • Magnesium • Phenol Red
Catalog: 20012-068
Size: 10 x 500 ml

MEM Vitamin Solution (100X), Liquid

Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Vitamin Solutions

Gibco® MEM Vitamin Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Vitamin Solution is formulated to contain 100x the vitamins found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Vitamin Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001...
Catalog: 11120-052
Size: 100 ml

MEM Amino Acids Solution, 50X

Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Amino Acid Solutions

Gibco® MEM Amino Acids Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability. Gibco® MEM Amino Acids Solution is formulated to contain 50X the essential amino acids (except L-glutamine) found in the standard Minimum Essential Medium (MEM). The complete formulation is available. Product Use For in vitro diagnostic use. Dual-site cGMP Manufacturing and Quality System Gibco® MEM Amino Acids Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufactur...
Catalog: 11130-051
Size: 100 ml

Sodium Pyruvate 100 mM Solution

Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > Sodium Pyruvate

Sodium Pyruvate is commonly added to cell culture medium as an energy source in addition to glucose. Since the cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not necessary to supplement all cultures. However, if the cells have been grown in medium supplemented with sodium pyruvate, we recommend the supplementation is continued as the cells become accustomed to this addition and their growth will lag without it. Gibco® Sodium Pyruvate, 100 mM Solution is formulated as 11 g per liter of water. The final concentration of sodium pyruvate used in most cell...
Catalog: 11360-070
Size: 100 ml

SYTO® 82 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains

The cell-permeant SYTO 82 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.
Catalog: S11363
Size: 250 µl

50X Denhardt's Solution

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Blotting Buffers

Denhardt's Solution is a mixture of blocking agents used in membrane-based hybridization protocols. The solution contains 1% Ficoll (type 400), 1% polyvinylpyrrolidone, and 1% bovine serum albumin. Sterile filtered.
Catalog: 750018
Size: 100 ml

Acrylamide/Bis 19:1 40% (w/v) Solution

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Acrylamide & Polymerizing Agents

Ambion® Acrylamide/Bis 19:1 is a 40% (w/v) solution of acrylamide (38%) and bis-acrylamide (2%) ideal for use in ribonuclease protection assay, sequencing gels, and sizing DNA or RNA fragments. Supplied in one bottle containing 500 mL. The solution is provided in a ready-to-use form, reducing the dust, inhalation, and contact hazard associated with weighing and preparing acrylamide and bis-acrylamide powders and solutions. Concentrations are carefully controlled and all solutions are micro-filtered prior to packaging. All Ambion® acrylamides are rigorously tested for contaminating non-specific...
Catalog: AM9022
Size: 500 ml

SYTO® 60 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains

The cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.
Catalog: S11342
Size: 250 µl

Lysing Solution, Whole Blood Lysing Solution

Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents

Invitrogen Cal-Lyse™ is a lysing solution formulated to enable the lysis of erythrocytes in samples of anticoagulated human peripheral blood. Invitrogen Cal-Lyse™ lysing solution is intended to be used as an aid in the enumeration of leukocytes that have been stained with monoclonal antibodies for flow cytometric analysis
Catalog: GAS-010S-100
Size: 100 ml

TMB Solution (ELISA)

Category: Products & Services > Labeling & Detection Products > Enzyme Substrates > Chromogens

TMB (Tetramethyl Benzidine) chromogen solution may be used as a substrate for horseradish peroxidase (HRP) in ELISAs.
Catalog: 00-2023
Size: 500 ml

Pluronic® F-127 (20% Solution in DMSO)

Category: Products & Services > Buffers & Chemicals > Detergents & Solubilizers

Pluronic F-127 *20% solution in DMSO* is a nonionic, surfactant polyol (molecular weight approximately 12,500 daltons) that has been found to facilitate the solubilization of water-insoluble dyes and other materials in physiological media. Pluronic F-127 is commonly used to help disperse the acetoxymethyl (AM) esters of our ion indicators as well as our cell tracer dyes such as CFDA-SE.
Catalog: P-3000MP
Size: 1 ml

Bovine Albumin Fraction V Solution (7.5%)

Category: Products & Services > Cell Culture & Transfection Reagents > Supplements > BSA & Albumin Supplements

7.5 g/100 ml Prepared with Bovine Serum Albumin Fraction V in phosphate-buffered saline.
Catalog: 15260-037
Size: 100 ml

Hygromycin B, 50 mg/ml Solution

Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics

Hygromycin B is a water-soluble antibiotic purified from the bacterium Streptomyces hydroscopicus . Hygromycin B acts by binding to the 80S subunit of the bacterial ribosome and inhibiting protein synthesis. Gibco® Hygromycin B is used as a bacterial selective antibiotic in the concentration range of 100 - 1000 µg / ml. This product is supplied as a 50 mg / ml solution. Resistance to Hygromycin B is conferred by the E. coli hygromycin resistance gene ( hyg or hph ). Hygromycin B uses a different mode of action than Geneticin®, Blasticidin S, or Zeocin™, so it is excellent for use in dual-selec...
Catalog: 10687-010
Size: 20 ml

SYTOX® Green Nucleic Acid Stain - 5 mM Solution in DMSO

Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains

SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population. • Impermeant to live cells • Excitation/Emission: 504/523 nm • Use with 488 Argon-ion laser • Works with mammalian cells and both Gram-positive and Gram-negative bacteria Simple Viability Determination SYTOX® Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, ...
Catalog: S7020
Size: 250 µl

SDS, 20% Solution

Category: Products & Services > Buffers & Chemicals > Denaturing Agents

Ambion® molecular biology grade, SDS, 20% Solution is supplied in one bottle containing 250 mL. The buffer is certified RNase-free, economical, and ready-to-use. SDS is used as a component of both hybridization and wash buffers for northern and Southern blots and has many other uses around the lab.
Catalog: AM9820
Size: 250 ml

Fixative-Free Lysing Solution, High-Yield Lyse

Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents

High-Yield Lyse is a premixed, fixative-free erythrocyte lysing solution that can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with Invitrogen's fluorochrome-conjugated monoclonal antibodies.
Catalog: HYL-250
Size: 500 ml

Pro-Q® Diamond Phosphoprotein Gel Destaining Solution, 1 L

Category: Products & Services > Labeling & Detection Products > Gel & Membrane Stains

Pro-Q Diamond phosphoprotein gel stain provides a convenient method for selectively staining phosphoproteins in acrylamide gels, without the need for blotting or phosphoprotein specific antibodies and Western blot analysis. Preparation of the Pro-Q Diamond phosphoprotein gel destaining solution is described in the gel staining protocol, but we also provide the detaining solution premixed for your convenience.
Catalog: P-33310
Size: 1 L

Alsever's Solution

Category: Products & Services > Buffers & Chemicals > General Buffers > Other General Buffers

This reagent is intended for the preparation of positive and negative controls from cord blood for Fetal Hemoglobin (HbF) testing. Class: GPR Statement: This product is for GPR use. Preservative: None
Catalog: HFH-11
Size: 100 ml