Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Chemokine (C-C motif) Ligand 20 (CCL20), a recombinant mouse protein, is supplied in frozen format and is quite suitable for use in protein structure analyses. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse CCL20 (O89093-1) (Met 1-Met 97) that is fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Reconstitution: No recons...
Catalog: 50048-M08B-25
Size: 5 × 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Chemokine (C-C motif) Ligand 20 (CCL20), a recombinant mouse protein, is supplied in frozen format and is quite suitable for use in protein structure analyses. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse CCL20 (O89093-1) (Met 1-Met 97) that is fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Reconstitution: No recons...
Catalog: 50048-M08B-5
Size: 5 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Chemokine (C-C motif) Ligand 20 (CCL20), recombinant mouse protein is supplied in frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse CCL20 (O89093-1) (Met 1-Met 97) fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Reconstitution: No reconstitution neede...
Catalog: 50048-M08B-250
Size: 5 × 50 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Growth Factors, Chemokines & Cytokines
Chemokine (C-C motif) Ligand 20 (CCL20), recombinant mouse protein is supplied in frozen protein. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. This recombinant protein was expressed from a DNA sequence encoding the mouse CCL20 (O89093-1) (Met 1-Met 97) fused to a polyhistidine tag at the C-terminus. Activity: This recombinant protein has not been tested. Formulation: 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Reconstitution: No reconstitution neede...
Catalog: 50048-M08B-50
Size: 50 µg
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Protein Gels > Tris-Acetate Gels
NuPAGE® Novex Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended Buffers: Run NuPAGE® Novex Tris-Acetate gels...
Catalog: EA03785BOX
Size: 1 box
Category: Products & Services > Buffers & Chemicals > General Chemicals > Other General Chemicals
Ambion® Molecular biology grade, 3 M Sodium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for cont...
Catalog: AM9740
Size: 100 ml
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Protein Gels > 2-D Gels
NuPAGE® Novex Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended Buffers: Run NuPAGE® Novex Tris-Acetate gels...
Catalog: EA0376BOX
Size: 1 box
Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains
DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells. DiI is also available as a solution (V-22885), as a paste (N-22880) or as a solid (D-282).
Catalog: D-3911
Size: 25 mg
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Voltage & Membrane Potential Sensors
Voltage Sensor Probes (VSPs) are a Fluorescence Resonance Energy Transfer (FRET)-based voltage-sensing assay technology used to measure changes in cellular membrane electrical potential. VSPs are ideal for screening compounds that modulate ion channels because they provide: High-throughput capabilities-screen 32,000 samples in one day Highly sensitive detection-accurately and precisely report a 1% change in the Emission Ratio per mV Rapid detection response-sub-second time response Ratiometric readout (the Emission Ratio)-reduces experimental errors arising from well-to-well variations in cell...
Catalog: K1022
Size: 5 mg
Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Reactive Oxygen & Nitrogen Species
MTT is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble MTT to an insoluble purple formazan. The formazan is then solubilized and its concentration determined by optical density.
Catalog: M-6494
Size: 1 g
Category: Products & Services > Labeling & Detection Products > Crosslinkers > Heterobifunctional Crosslinkers
EDAC is a water-soluble carbodiimide that can be used to crosslink biological substances that contain carboxylate acids and primary amines. Protocols for crosslinking molecules with this reagent can be found in Bioconjugate Techniques , by G.T. Hermanson (B-7884).
Catalog: E-2247
Size: 100 mg
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Amine-Reactive Fluorophores, Biotins, Quantum Dots, & Other Labels
The amine-reactive PyMPO succinimidyl ester can be used to create bioconjugates with this environment-sensitive fluorophore.
Catalog: S-6110
Size: 5 mg
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > Fluorescently Labeled Nucleotides
The mixed-isomer analog of BODIPY® FL GDP is comprised of the BODIPY® FL fluorophore attached to the 2’ or 3’ position of the ribose ring via an aminoethylcarbamoyl linker. BODIPY® FL exhibits spectral characteristics similar to fluorescein (FITC) or the Alexa Fluor® 488 dye.
Catalog: G-22360
Size: 100 µl
Category: Products & Services > Gels, Fractionation Strips, & Membranes > Blotting Membranes > iBlot® Detection & Transfer Stacks
This product contains three boxes of 10 sets/box of iBlot® Transfer Stack, Mini, Nitrocellulose (Cat# IB3010-02) The iBlot® Dry Blotting System efficiently and reliably transfers proteins from polyacrylamide gels in seven minutes or less without the need for buffers or an external power supply. A self-contained unit (Figure 1), the iBlot® device uses disposable blotting stacks with integrated transfer membranes (nitrocellulose and PVDF), offering the convenience of a bufferless, plug-and-play system. Compared to conventional blotting techniques, the iBlot® system enables higher downstream dete...
Catalog: IB3010-32
Size: 3 x 10 sets⁄box
Category: Products & Services > Oligos, Primers, Probes & Nucleotides > Nucleotides > Fluorescently Labeled Nucleotides
BODIPY FL adenosine 5'-triphosphate (A-12410) and BODIPY FL guanosine 5'-triphosphate are composed of a BODIPY FL fluorophore attached to the 2' or 3' position of the ribose ring via a linker. Interactions between the fluorophore and the purine base are evident from the spectroscopic properties of these nucleotide analogs. The fluorescence quantum yield of BODIPY FL GTP is only about one-tenth that of BODIPY FL ATP; however, this yield may be enhanced upon binding of BODIPY FL GTP to GTP-binding proteins.
Catalog: G-12411
Size: 100 µl
Category: Products & Services > Slide & Chip Arrays
NATIVEPAGE 3-12% GELS, 10-WELL
Catalog: BN2011BX10
Size: 1 each
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Apoptosis Kits
The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The kit includes the novel fluorogenic substrate CellEvent® Caspase-3/7 Green Detection Reagent, as well as SYTOX® AADvanced™ Dead Cell Stain. • Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7 • Easy identification—clearly identify live, dead, and apoptotic cell populations • Quick analysis—washing and fixation is not required • Multicolor compatibility—combine with other dyes excitable by the 488 n...
Catalog: C10427
Size: 100 assays
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
The casein kinase I (CKI) gene family encodes serine⁄threonine kinases that preferentially phosphorylate acidic substrates. CKI proteins are monomeric and range from 25 to 55 kD. They are ubiquitous, being found in the nuclei, cytoplasm, and membrane fractions of eukaryotic cells
Catalog: PR7474A
Size: 100 µg
Category: Products & Services > Proteins, Enzymes, & Peptides > Kinases > Serine⁄Threonine Kinases
The casein kinase I (CKI) gene family encodes serine⁄threonine kinases that preferentially phosphorylate acidic substrates. CKI proteins are monomeric and range from 25 to 55 kD. They are ubiquitous, being found in the nuclei, cytoplasm, and membrane fractions of eukaryotic cells
Catalog: PV3991
Size: 1 mg
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Apoptosis Kits
CellEvent® Caspase-3/7 Green ReadyProbes™ Reagent is a fluorogenic, no-wash indicator of activated caspase-3/7 for live- and fixed-cell applications. Activation of caspase-3 is an early indicator of apoptosis and CellEvent® Caspase-3/7 Green reagent allows rapid and sensitive detection of cells destined for cell death. Like other ReadyProbes™ reagents, CellEvent™ Caspase-3/7 Green reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells. CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent provides...
Catalog: R37111
Size: 1 kit