Gpcr Cell Based Assay Kits
For Academic pricing please login or contact us at discoverysciences@invitrogen.com. The Tango™ CCR5- bla U2OS cells contain the human Chemokine (C-C Motif) Receptor 5 (CCR5) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR- bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase ( bla ) reporter gene under the control of a UAS response element. The Tango™ CCR5- bla U2OS cells are functionally validated for Z' and EC 50 concentrations of MIP-1a. In addition,...
Catalog: K1788
Size: 8 × 10^6 cells
Size: 8 × 10^6 cells
The Vybrant® Cell Adhesion Assay Kit is a fast and sensitive assay for measuring cell-cell or cell-surface adhesion for a variety of cell types. In this assay, cells are labeled with calcein AM and allowed to adhere. After removal of nonadherent cells, calcein fluorescence is used to calculate the number of adherent cells.
Catalog: V-13181
Size: 1 kit
Size: 1 kit
The Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit contains a sensitive, one-step assay that uses the Amplex® Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) to detect hydrogen peroxide (H 2 O 2 ) or peroxidase activity. The Amplex® Red reagent, in combination with horseradish peroxidase (HRP), has been used to detect H 2 O 2 released from biological samples, including cells, or generated in enzyme-coupled reactions. Furthermore, Amplex® Red reagent can be used as an ultrasensitive assay for peroxidase activity when H 2 O 2 is in excess. In the presence of peroxidase, the Amplex® Red reag...
Catalog: A22188
Size: 1 kit
Size: 1 kit
Th e GeneBLAzer® Jurkat Control Kit contains constitutively expressing (CMV- bla ) and wildtype Jurkat cells to provide positive and negative controls, respectively, for evaluating beta-lactamase activity (Figure 1).
Catalog: K1206
Size: 1 kit
Size: 1 kit
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4381: The LanthaScreen™ TR-FRET Androgen Receptor (AR) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential AR ligands as agonists of coactivator recruitment or antagonists of agonist-dependent coactivator recruitment. In both the agonist and antagonist modes, the homogeneous mix-and-read assay uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a rat AR ligand-binding domain (AR-LBD) that is tagged with hexahisti...
Catalog: A15119
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4836: The LanthaScreen™ TR-FRET Constitutive Androstane Receptor (CAR) Coactivator Assay provides a sensitive and robust method for highthroughput screening (HTS) of potential CAR ligands as agonists of ligand-dependent coactivator recruitment or inverse agonists of liganddependent coactivator displacement. The kit uses a terbium-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a CAR ligand-binding domain (CAR-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneo...
Catalog: A15141
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4544: The LanthaScreen™ TR-FRET ER alpha Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential estrogen receptor (ER) alpha ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an ER alpha ligand-binding domain (ER alpha-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format. Agonist m...
Catalog: A15125
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4541: The LanthaScreen™ TR-FRET Estrogen Receptor beta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ER beta ligands as agonists or antagonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a ER beta ligand-binding domain (ER beta-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format. Agonist mode To run...
Catalog: A15124
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4663: The LanthaScreen™ ERR (estrogen related receptor) alpha TR-FRET Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential ERR alpha ligands as inverse agonists (sometimes referred to as antagonists) of coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an ERR alpha ligand-binding domain (ERR alpha-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-rea...
Catalog: A15130
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4800: The LanthaScreen™ TR-FRET ERR (estrogen related receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential ERR beta ligands as inverse agonists of ligand-dependent coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant ERR beta ligand-binding domain (ERR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay...
Catalog: A15139
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4408: The LanthaScreen™ TR-FRET Estrogen Related Receptor (ERR) gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ERR gamma ligands as antagonists/inverse agonists of coactivator displacement. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (Figure 1). How it works The LanthaScreen™ TR...
Catalog: A15121
Size: 800 × 20 µl assays
Size: 800 × 20 µl assays
Gpcr Cell Based Assay Kits
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