Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Trypsin
Gibco® Trypsin 1:250 powder is an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength it is widely used for cell dissociation, during routine cell culture passaging, and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing Quality Testing Gibco® Trypsin 1:250 is tested for endotoxin, mycoplasma, bacterial, fungal and viral contamination. Other quality tests include multiple activity assays, ash analysis and mois...
Catalog: 27250-018
Size: 100 g
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • Low Glucose • L-glutamine • Sodium Pyruvate • Phenol Red • HEPES
Catalog: 11054-001
Size: 1 L
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES
Catalog: 11960-069
Size: 10 × 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
Formulation per one liter: 10 g SELECT Peptone 140 5 g SELECT Yeast Extract 5 g sodium chloride
Catalog: 12780-029
Size: 2.5 kg
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • Sodium Pyruvate • L-glutamine • HEPES • Phenol Red
Catalog: 11965-126
Size: 6 × 1000 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Trypsin Inhibitors
Soybean Trypsin Inhibitor is one of several protease inhibitors found in soybeans. Trypsin is inhibited at a molar ratio of 1:1, while chymotrypsin and plasmin are inhibited to a lesser extent. Soybean trypsin inhibitor will also inhibit other serine proteases. Gibco® Soybean Trypsin Inhibitor, powder has an activity greater than 7,000 BAEE units per mg. A 0.5 mg/mL Soybean Trypsin Inhibitor stock solution should be made by dissolving the non-sterile powder in 0.01M Borate Buffer or Dulbecco's Phosphate Buffered Saline (DPBS), without calcium and magnesium. Filter sterilize the stock solution ...
Catalog: 17075-029
Size: 1 g
Category: Products & Services > Cell Culture & Transfection Reagents > Microbiological Media > Bacterial Media
Formulation per one liter: 10 g SELECT Peptone 140 5 g SELECT Yeast Extract 10 g sodium chloride
Catalog: 12795-027
Size: 500 g
Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS
Catalog: 00-3000
Size: 12 × 1 L
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES • L-methio...
Catalog: 21013-024
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • Low Glucose • HEPES • Sodium Pyruvate • GlutaMAX™ • Phenol Red
Catalog: 10567-014
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > Iscove's (IMDM)
Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. Life Technologies offers a variety of Gibco® IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This IMDM is modified as follows: With Without • L-glutamine • α-thioglycerol • Phenol Red • 2-mercaptoethanol
Catalog: 12200-036
Size: 10 × 1 L
Category: Products & Services > Cell Culture & Transfection Reagents > Antibiotics & Antimycotics
Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg / ml for bacteria, or 200 – 500 µg / ml for most mammalian cells. This product is supplied as a powder and should be m...
Catalog: 11811-031
Size: 5 g
Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Collagenase
Collagenase is a protease with specificity for the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Glyc-Pro. This sequence is found in high frequency in collagen. Collagenase is unique among proteases in its ability to degrade the triple-helical native collagen fibrils commonly found in connective tissues such as skin, tendon, blood vessels, and bone. Collagenase disaggregation has been suitable for the culture of human tumors, mouse kidney, human adult and fetal brain, and many other tissues including epithelium. It is relatively gentle, dissociates well at physiologic...
Catalog: 17101-015
Size: 1 g
Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Other Cell Dissociation Reagents
Dispase II (neutral protease) is an amino-endo peptidase that hydrolyses N-terminal peptide bonds of non-polar amino acid residues. This sequence is found in high frequency in collagen. Dispase II has a mild proteolytic action that makes it useful for the isolation and routine passaging of primary cells. It is relatively gentle, dissociates well at physiological temperature and pH, and generally maintains cell membrane integrity. Gibco® Dispase II is produced in Bacillus polymyxa and packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation and to prevent cl...
Catalog: 17105-041
Size: 5 g
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES
Catalog: 31053-028
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Basal Media > DMEM Media
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This DMEM is modified as follows: With Without • Low Glucose • HEPES • L-Glutamine • Sodium Pyruvate • Phenol Red
Catalog: 11885-092
Size: 10 × 500 ml