General Chemicals Nucleic Acid Precipitation Solutions
Ambion® GlycoBlue™ consists of a blue dye covalently linked to glycogen, a branched chain carbohydrate and is useful as a nucleic acid coprecipitant. Ideal coprecipitant in nuclease protection assays at 1/100 dilution of stock solution, it is provided in five tubes of 0.3 mL each at a concentration of 15 mg/mL. • Ideal for RT-PCR • Increases pellet mass • Quantitative recovery of low concentrations (ng/mL) of nucleic acid • Prevents pellet loss in nuclease protection assays What is a Coprecipitant? Coprecipitants are inert substances used to aid recovery of nucleic acids during alcohol precipi...
Catalog: AM9516
Size: 5 × 300 µl
Size: 5 × 300 µl
The cell-permeant SYTO 82 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.
Catalog: S11363
Size: 250 µl
Size: 250 µl
The cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.
Catalog: S11342
Size: 250 µl
Size: 250 µl
SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population. • Impermeant to live cells • Excitation/Emission: 504/523 nm • Use with 488 Argon-ion laser • Works with mammalian cells and both Gram-positive and Gram-negative bacteria Simple Viability Determination SYTOX® Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, ...
Catalog: S7020
Size: 250 µl
Size: 250 µl
The Ambion® RecoverAll™ Total Nucleic Acid Isolation Kit is for the extraction of total nucleic acid from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. Sufficient reagents are included for 40 purifications from up to four 20 µm sections, or up to 35 mg of unsectioned core samples each. Features of the RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE: • Optimized for isolation of total nucleic acids, including microRNAs, from FFPE tissue • No overnight Proteinase K digestion required—deparaffinize in the morning and perform qRT-PCR in the afternoon • Typical yields are...
Catalog: AM1975
Size: 40 purifications
Size: 40 purifications
THE Ambion® RNA Storage Solution is a buffer that provides greater RNA stability than standard 0.1 mM EDTA or TE Buffer. This 1 mM sodium citrate, pH 6.4 +/- 0.2 buffer is provided in one bottle containing 50 mL. The last step in every RNA isolation protocol is to resuspend the purified RNA pellet. After painstakingly isolating the RNA, it is crucial that the pellet be suspended and stored in a safe, RNase-free solution. THE RNA Storage Solution has two features that minimize base hydrolysis of RNA: a low pH, and sodium citrate, which is an efficient chelating agent. THE RNA Storage Solution i...
Catalog: AM7001
Size: 50 ml
Size: 50 ml
Ambion® RNaseZap® is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap® onto the surface to be decontaminated and rinse it off with RNase-free water. Supplied in a single 4 liter cubitainer. • Completely removes RNase contamination from glass and plastic surfaces • Excels at removing high levels of RNase contamination whereas similar products fail • Proven effective at removing high concentrations of dried-on RNase A • Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free Working with RNA requires that special measures be...
Catalog: AM9784
Size: 4 L
Size: 4 L
RNase AWAY® Reagent is a ready-to-use solution for eliminating RNase and DNA contamination from labware. Apply it evenly over the surface of glassware or plasticware to be treated and then rinse it away with distilled water. Unwanted RNase and DNA contamination are eliminated. RNase AWAY® Reagent is nonabrasive, noncarcinogenic, and nonbiologically corrosive. Performance and Quality Testing: RNase AWAY® Reagent is functionally tested for the elimination of RNase activity and DNA. No detectable RNase activity or DNA is observed.
Catalog: 10328-011
Size: 250 ml
Size: 250 ml
Yeast tRNA is suitable for use as a carrier in nucleic acid purification and precipitation procedures. It is supplied in lyophilized form.
Catalog: 15401-011
Size: 25 mg
Size: 25 mg
Ambion® RNA secure ™ (patents pending) is a unique non-enzymatic reagent that will irreversibly inactivate RNases in solution. 10 mL supplied. • No post-treatment autoclaving • Safe, easy-to-use reagent inactivates RNases in solutions • Inactivation process can be repeated to protect against newly introduced contaminants • Compatible with downstream procedures, such as RT-PCR and in vitro transcription • Inactivate RNases in Tris and other solutions that cannot be treated with DEPC RNase contamination in reagents used for RNA isolation and analysis can contribute to experimental inconsistency ...
Catalog: AM7006
Size: 10 ml
Size: 10 ml
Ambion® Carrier RNA (1 mg⁄mL) is a component of all the MagMAX™ Isolation Kits that may be purchased separately. Provided in one tube of 550 μL.
Catalog: 4382878
Size: 500 µl
Size: 500 µl
Ambion® DNA Zap ™ (patent pending) consists of two solutions that are innocuous when used alone, but become a potent nucleic acid degrading solution when mixed. This mixture is able to instantaneously degrade high levels of contaminating DNA and RNA from surfaces. 250 mL of each solution are provided. • Completely degrades contaminating DNA and RNA at the level of PCR sensitivity • Works on contact • Degrades nucleic acid, unlike other products on the market that only act as detergents • Ideal for cleaning PCR tubes, PCR machine surfaces, pipettors, lab benches, lab equipment, and microfuge tu...
Catalog: AM9890
Size: 250 ml
Size: 250 ml
Ambion® RNaseAlert® QC System is a patent-pending technology detects RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. Contain sufficient reagents for 5 × 96 high throughput assays. • Detects as little as 3.5 x 10 -7 units (~0.5 pg) of RNase A with a fluorometer • Simple, straightforward 30 min assay • Monitor RNase activity in column fractions during protein purification • Use with DNaseAlert™ QC System to quantitatively detect both RNases and DNases in the same sample The RNaseAlert® QC System uses a novel RNA substrate tagged with a fluoresc...
Catalog: AM1966
Size: 480 assays
Size: 480 assays
Ambion® DNA Zap ™ (patent pending) consists of two solutions that are innocuous when used alone, but become a potent nucleic acid degrading solution when mixed. This mixture is able to instantaneously degrade high levels of contaminating DNA and RNA from surfaces. 250 mL of each solution are provided. • Completely degrades contaminating DNA and RNA at the level of PCR sensitivity • Works on contact • Degrades nucleic acid, unlike other products on the market that only act as detergents • Ideal for cleaning PCR tubes, PCR machine surfaces, pipettors, lab benches, lab equipment, and microfuge tu...
Catalog: AM9890M
Size: 250 ml (Manual)
Size: 250 ml (Manual)
RNAlater®-ICE is a novel reagent for transitioning frozen tissue to a state that is easily processed for extraction of high quality RNA. Frozen tissues are simply submerged in RNAlater®-ICE and allowed to thaw overnight at -20°C. Once thawed, the tissues can be processed like fresh tissues using standard RNA isolation procedures. No more laborious grinding of frozen tissue to safeguard the RNA. • Process previously frozen tissues like freshly harvested samples • Thawing tissues in RNAlater®-ICE protects RNA from degradation • No more tissue pulverizing with mortar and pestle and awkward transf...
Catalog: 4427575
Size: 10 bottles
Size: 10 bottles
RNaseAlert® Lab Test Kit v2 detects RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. This kit is suitable for testing small numbers of sample and ensuring that solutions, tubes, tips, etc. in your lab are RNase-free. This new version contains a more sensitive substrate that is ~60% more sensitive than the previous version. The kit contains sufficient reagents for 25 reactions. • Detects as little as ~0.3 pg of RNase A with a fluorometer • Simple, straight-forward 30-minute assay The RNaseAlert® Lab Test Kit v2 uses a new, more sensitive, novel...
Catalog: 4479768
Size: 25 reactions
Size: 25 reactions
The RNaseAlert® QC System v2 detects RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. This version contains a substrate that is ~60% more sensitive than the previous version. The kit contains sufficient reagents for 5 x 96 (480) high-throughput assays. Features of RnaseAlert® QC System v2: • Detects as little as ~0.3 pg of RNase A with a fluorometer • Simple, straight-forward, 30-minute assay • Designed to monitor RNase activity in column fractions during protein purification • Use with DNaseAlert™ QC System to quantitatively detect both RNase...
Catalog: 4479769
Size: 480 reactions
Size: 480 reactions
The high-affinity nucleic acid stain BOBO™-1 has excitation/emission maxima ~462/481 nm when bound to double-stranded DNA in aqueous solution. This cyanine dimer is cell-impermeant.
Catalog: B3582
Size: 200 µl
Size: 200 µl
Acid Phenol:Chloroform:IAA (125:24:1) is premixed and supplied at pH 4.5 ± 0.2. Provided in one bottle of 400 mL. In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when mixing. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality-tested, saturated phenols are ready to use, eliminating handling problems and offering a more convenient, safer, an...
Catalog: AM9722
Size: 400 ml
Size: 400 ml
General Chemicals Nucleic Acid Precipitation Solutions
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