General Buffers Antibody Diluent

Buffer Kit (100 ml Each)

Category: Products & Services > Buffers & Chemicals > General Buffers > Other General Buffers

The Ambion® Buffer Kit provides high quality, RNase free buffers for critical RNA analysis procedures. The kit consists of one 100 mL bottle of each of the following solutions: 1 M Tris pH 7.0, 1 M Tris pH 8.0, 5 M NaCl, 1 M MgCl 2 , 0.5 M EDTA pH 8.0, 2 M KCl, 5 M Ammonium Acetate, and DEPC-treated H 2 O. Ammonium Acetate is sterile filtered. All other solutions are autoclaved post-packaging to ensure sterility. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
Catalog: AM9010
Size: 1 set

PBS - Phosphate-Buffered Saline Tablets

Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS

Catalog: 00-3002
Size: 100 tablets

1X TE Buffer

Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers

The 1X TE Buffer is a component in the PureLink™ 96 Plasmid Purification System. The 1X TE buffer is used to resuspend the final purified plasmid pellet and contains very low EDTA, so it is compatible with sequencing and other enzymatic applications.
Catalog: 12090-015
Size: 100 ml

PBS - Phosphate-Buffered Saline Powder (12 X 1 L)

Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS

Catalog: 00-3000
Size: 12 × 1 L

PBS - Phosphate-Buffered Saline 10X, pH 7.4

Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS

Molecular biology grade, Ambion® PBS 10X, pH 7.4 solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminatin...
Catalog: AM9625
Size: 1 L

PBS - Phosphate-Buffered Saline 10X, pH 7.4

Category: Products & Services > Buffers & Chemicals > General Buffers > PBS (Non-Cell Culture) & TBS

Molecular biology grade, Ambion® PBS 10X, pH 7.4 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contamina...
Catalog: AM9624
Size: 500 ml

20X TE Buffer, RNase-free

Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers

The RNase-free TE buffer concentrate, 20X TE buffer , which is essential to the success of the RiboGreen® RNA quantitation assays (R11491, R37302) is available separately from the kits to extend the number of low-concentration assays.
Catalog: T11493
Size: 100 ml

HEPES, 1M Buffer Solution

Category: Products & Services > Buffers & Chemicals > General Buffers > HEPES Buffer

Gibco® HEPES (N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10 – 25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO 2 incubator. HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorbance. Product Use For in vitro diagnostic use. Dual-site cGMP...
Catalog: 15630-130
Size: 20 × 100 ml

1 M Tris, pH 7.0

Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers

Ambion® Molecular biology grade, 1M Tris, pH 7.0 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contamina...
Catalog: AM9851
Size: 500 ml

Alsever's Solution

Category: Products & Services > Buffers & Chemicals > General Buffers > Other General Buffers

This reagent is intended for the preparation of positive and negative controls from cord blood for Fetal Hemoglobin (HbF) testing. Class: GPR Statement: This product is for GPR use. Preservative: None
Catalog: HFH-11
Size: 100 ml

UltraPure™ 1M Tris-HCI, pH 7.5

Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers

UltraPure™ 1 M Tris-HCl Buffers are pre-mixed, pH-adjusted, sterile-filtered solutions. Prepared as 1 M concentrates, these buffers can be diluted to the desired concentration and used in molecular biology or general biochemistry applications. Performance and Quality Testing: No DNase, RNase, or protease activity detected
Catalog: 15567-027
Size: 1 L

UltraPure™ 1M Tris-HCI, pH 8.0

Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers

UltraPure™ 1 M Tris-HCl Buffers are pre-mixed, pH-adjusted, sterile-filtered solutions. Prepared as 1 M concentrates, these buffers can be diluted to the desired concentration and used in molecular biology or general biochemistry applications. Performance and Quality Testing: No DNase, RNase, or protease activity detected
Catalog: 15568-025
Size: 1 L

Solution D

Category: Products & Services > Buffers & Chemicals > General Buffers > Other General Buffers

Solution D is an anion transport inhibitor, for cells such as CHO-K1, that can be used in conjunction with CCF2-AM and CCF4-AM-based studies to prevent active cellular export of the FRET-based substrates after loading.
Catalog: K1156
Size: 1 ml

20X SSC

Category: Products & Services > Buffers & Chemicals > General Buffers > SSC Buffers

Molecular biology grade, Ambion® 20X SSC solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating non...
Catalog: AM9770
Size: 500 ml

Water Saturated Phenol, pH 6.6 (Raise to pH 7.9 with included Buffer)

Category: Products & Services > Buffers & Chemicals > General Chemicals > Other General Chemicals

Ambion® Water Saturated Phenol is supplied premixed and at pH 6.6 (pH 7.9 ± 0.2, with included buffer). Water Saturated Phenol is used in RNA and DNA extraction procedures and is provided in one bottle of 400 mL. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more convenient, safer, and easier alternative to preparing solutions from crystalline phenol.
Catalog: AM9712
Size: 400 ml

Phenol:Chloroform:IAA, 25:24:1, pH 6.6 (raise to pH 7.9 with Included Buffer)

Category: Products & Services > Buffers & Chemicals > General Chemicals > Other General Chemicals

Ambion® Acid Phenol:Chloroform:IAA (25:24:1) is premixed and supplied at pH 6.6 (pH 7.9 ± 0.2), with included buffer. Acid Phenol:Chloroform:IAA is provided in one bottle of 100 mL. In RNA and DNA extraction procedures, Acid Phenol:Chloroform:IAA (pH 7.9) helps to stabilize the interface and prevents foaming when mixing. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more conv...
Catalog: AM9730
Size: 100 ml

Histidine Tag (6xHis) Mouse Monoclonal Antibody (clone 3D5) HRP Conjugate

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Epitope Tag & Reporter Specific Antibodies

This purified Mouse Histidine (C-term) Tag Monoclonal Antibody recognizes a polyhistidine amino acid sequence at the carboxy-terminus of proteins. The free carboxy-terminus of the terminal histidine residue is a key element of the epitope recognition site of the anti-His (C-term) Antibody. The Anti-His(C-term) Monoclonal Antibody recognizes the sequence: -His-His-His-His-His-His-COOH . Validated applications for Histidine (C-term) Tag monoclonal antibody are western blotting and ELISA. Antibody Specifications: • Applications: Validated applications for Histidine (C-term) Tag Monoclonal Antibod...
Catalog: R931-25
Size: 50 µl

BigDye® Terminator v1.1 & v3.1 5X Sequencing Buffer

Category: Products & Services > Buffers & Chemicals > Sequencing Buffers & Reagents

This sequencing buffer is optimized for use with BigDye® Terminator v1.1 and v3.1 Cycle Sequencing Kits. For Research Use Only. Not for use in diagnostics procedures.
Catalog: 4336701
Size: 233 ml

ErbB-2 Mouse Monoclonal Antibody (clone e2-4001)

Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies

This purified Mouse anti-ErbB-2 Monoclonal Antibody is specific to human, rat, and mouse ErbB2 (HER2). Over-expression of c-erbB-2 is observed in 15–40% of primary human breast tumors and correlates with poor prognosis in node positive breast cancer. Over-expression of c-erbB-2 is also observed in ovarian, gastric, salivary, and non-small cell lung carcinomas. Validated applications for mouse anti-ErbB-2 monoclonal antibody are immunoprecipitation, immunohistochemistry, flow cytometry, and Western blotting. Antibody Attributes: • Applications: Validated applications for Mouse anti-ErbB-2 monoc...
Catalog: AHO1011
Size: 100 µg

Gel Loading Buffer II (Denaturing PAGE)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Loading Buffers

A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue. Supplied in one 1.4 mL tube. All Ambion® Gel Loading Solutions are rigorously tested for non-specific endonuclease activity, exonuclease activity, RNase activity, and for functionality. These are the same solutions we use in-house and in our kits.
Catalog: AM8546G
Size: 1 ml