Gene Synthesis
SuperScript® First-Strand Synthesis System for RT-PCR is optimized for sensitive RT-PCR from low-input RNA, Taq or Platinum® Taq DNA Polymerase for higher specificity PCR or with AccuPrime™ Pfx DNA Polymerase for high-fidelity cloning applications. The SuperScript® First-Strand Synthesis System for RT-PCR: Is optimized for RT-PCR Uses SuperScript® II RT for greater first-strand cDNA yields Detects as little as 50 to 100 molecules of RNA template from only 1 pg of total RNA (Figure 1) Includes oligo(dT) and random hexamers to prime cDNA synthesis for flexible use Amplifies first-strand cDNA pro...
Catalog: 11904-018
Size: 50 reactions
Size: 50 reactions
The SuperScript® Double-Stranded cDNA Synthesis Kit contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality double-stranded cDNA from total RNA or poly A + selected RNA (mRNA). The advanced capabilities of SuperScript® II Reverse Transcriptase, with its reduced RNase H activity, maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis. Features of the SuperScript® Double-Stranded cDNA Synthesis Kit include: • Reliability —Each buffer, reagent, and enzyme in the kit is of the same high quality you expect from Life Technologie...
Catalog: 11917-010
Size: 10 reactions
Size: 10 reactions
The SuperScript® III First-Strand Synthesis System for RT-PCR provides all the necessary components for synthesis of high-quality first-strand cDNA from total or poly(A)+ RNA. Used with PCR, this functionally tested system enables you to detect rare messages and generate enough material for cloning. The SuperScript® III First-Strand Synthesis System: Uses SuperScript® III RT for higher cDNA yields (Figure 1) Includes all components needed for first-strand cDNA synthesis Consists of optimized reagents for more robust performance Contains a streamlined protocol Detects template molecules from as...
Catalog: 18080-051
Size: 50 reactions
Size: 50 reactions
The SuperScript® III First-Strand Synthesis SuperMix provides high yields of first-strand cDNA in a convenient high-throughput supermix format. The simple, time-saving reaction set-up uses just two tubes-a 2X Reaction Mix and a SuperScript® III Enzyme Mix (Figure 1). An optional annealing buffer is provided for improved yields and sensitivity. The Enzyme Mix includes SuperScript® III Reverse Transcriptase to ensure higher cDNA yields, superior sensitivity-down to 0.1 pg (Figure 2)-and specificity, and compatibility with a wide variety of RT-PCR applications.
Catalog: 18080-400
Size: 50 reactions
Size: 50 reactions
The NCode™ VILO™ miRNA cDNA Synthesis Kit is part of a novel quantitative assay that provides flexibility, sensitivity, and specificity when quantitating miRNA expression. The system combines a carefully optimized polyadenylation reaction with the market-leading reverse transcriptase, SuperScript® III RT, in a universal first-strand cDNA synthesis reaction. Tried and tested SuperScript® III Reverse Transcriptase is formulated in an enhanced buffer system to provide you with the most reliable firststrand synthesis and higher cDNA yields, with sensitivity and accurate quantitation from 10pg to 1...
Catalog: A11193-050
Size: 50 reactions
Size: 50 reactions
Get precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems® TaqMan® Gene Expression Master Mix. TaqMan® Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend. TaqMan® Gene Expression Master Mix has been validated with TaqMan® Gene Express...
Catalog: 4369542
Size: 2000 reactions
Size: 2000 reactions
Custom TaqMan® Gene Expression Assay, LG
Catalog: 4332079
Size: 2900 reactions
Size: 2900 reactions
GeneArt® Synthetic Gene 1.8 kb SuperSPEED Service (Mark-up)
Catalog: 810154DE
Size: 1 each
Size: 1 each
The Click-iT® HPG Alexa Fluor® 488 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor® 488 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 488 azide leads to a chemoselective ligation or "click" reaction between the...
Catalog: C10428
Size: 1 kit
Size: 1 kit
The Click-iT® HPG Alexa Fluor® 594 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor® 594 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor® 594 azide leads to a chemoselective ligation or “click" reaction between the...
Catalog: C10429
Size: 1 kit
Size: 1 kit
Same as TaqVet Schmallenberg Virus - S Gene - Real-Time PCR Kit (SBVS/50) TaqMan® probes - RNA - duplex - One well reaction Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals. The LSI VetMAX™ kits include: • 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT en...
Catalog: SBVS50
Size: 50 tests
Size: 50 tests
The TaqMan® Human Immune Array from Applied Biosystems® is an efficient, easy-to-use micro fluidic card for quantitative gene expression analysis of targets known to have implications in immune response. This product is designed with 96 TaqMan® Gene Expression Assays loaded in singletons into the array’s 384 wells and is compatible with an upgraded Applied Biosystems 7900HT Fast Real-Time PCR System. The targets include cytokines, chemokines, growth factors, immune regulators, apoptosis markers, ischemia markers, tissue-specific markers, and others including classic endogenous markers.This pro...
Catalog: 4370573
Size: 4 cards
Size: 4 cards
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human p53 Signaling 96-well Plate contains 92 assays to p53 signaling associated genes and 4 assays to candidate endogenous control genes.
Catalog: 4418813
Size: 1 Fast plate
Size: 1 Fast plate
The Applied Biosystems® TaqMan® Array Human Androgens 96-well Plate contains 92 assays to androgen associated genes and 4 assays to candidate endogenous control genes. The panel of assays in the TaqMan® Array Human Androgens 96-well Plate targets genes which code for enzymes involved in androgen synthesis, regulators of androgen production, and receptors and signal transduction proteins which mediate their effects, and targets of androgen signaling. The panel also includes the Y-chromosome SRY genes, which play roles in male phenotype development, as well as differentiation of the bipotential ...
Catalog: 4414083
Size: 1 standard plate
Size: 1 standard plate
The Applied Biosystems® TaqMan® Array Human Transcription of tRNA 96-well Plate contains 28 assays to transcription of tRNA associated genes and 4 assays to candidate endogenous control genes. All assays are plated in triplicate. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. • Affordable; m...
Catalog: 4414196
Size: 1 standard plate
Size: 1 standard plate
The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of input total RNA in a single 20 µL reaction to single stranded cDNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Includes sufficient reagents for 1000 x 20 µL reactions. • Linear target amplification for real-time PCR • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost • 10-fold greater dynamic range than other kits cDNA from Total RNA The High Capacity cDNA Reverse Transcription Kit (for...
Catalog: 4374967
Size: 1000 reactions
Size: 1000 reactions
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Extracellular Matrix & Adhesion Molecules 96-well Plate contains 92 assays to extracellular matrix & adhesion molecules associated genes and 4 assays to candidate endogenous control genes.
Catalog: 4418778
Size: 1 Fast plate
Size: 1 Fast plate
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Transcription of mRNA 96-well Plate contains 44 assays to transcription of mRNA associated genes and 4 assays to candidate endogenous control genes. All assays are plated in duplicate.
Catalog: 4418839
Size: 1 Fast plate
Size: 1 Fast plate
Contains 45 mg 6-FAM™ Dye Phosphoramidite. 6-FAM™ Dye Phosphoramidite is used for the 5’ attachment of FAM™ dye during oligonucleotide synthesis. 6-FAM™ Dye Phosphoramidite is an optically pure isomer (not a mixture). The label is treated as if it were simply another base: It is inserted at the appropriate place in the sequence and incorporated automatically. • Specialty phosphoramidites allow online addition of the label during synthesis, saving time in production of labeled probes and primers • Designed and optimized for use with ABI™ synthesizers, guaranteeing reliable nucleic acid synthesi...
Catalog: 403169
Size: 45 mg
Size: 45 mg
This Applied Biosystems® kit provides the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, and oligo (dT) 16 , reverse primers are included for cDNA synthesis flexibility. Supplied as 2000 reactions in 10 tubes (200 reactions/tube). • Contains MultiScribe® Reverse Transcriptase, a recombinant Moloney Murine Leukemia Virus Reverse Transcriptase • Random hexamers and oligo (dT) 16 included for flexibility • Individual components provide versatility in assay set-up • Compatible with one-step or two-step RT-PCR reactions TaqMan® Reverse Transcription Reagents provide all...
Catalog: 4304134
Size: 2000 reactions
Size: 2000 reactions
Gene Synthesis
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