gel purification

PureLink® Quick Gel Extraction Kit

Category: Products & Services > Kits & Assays > Nucleic Acid Purification Kits & Reagents > Gel Extraction Kits

Successfully purify DNA fragments from 40 bp to 10 kb Proven purity made possible using traditional silica-based technology The PureLink® Gel Extraction Kit is designed to purify DNA fragments from agarose gels (1). The simple procedure uses a silica-based spin cartridge (Figure 1) to purify DNA fragments from 40 bp to 10 kb in in vitro transcription, restriction mapping, cloning, and labeling.
Catalog: K2100-12
Size: 50 preps

OWL Centipede™ Gel System

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Gel Boxes

• For use with SSP products or any application requiring agarose gel separation • Multiple formats available to meet various throughput needs • Includes replacement parts for each unit
Catalog: 80000-1D
Size: 1 each

E-Gel® Imager Camera Hood Kit

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Gel Imagers & Digital Electrophoresis Systems

The E-Gel® Camera Hood Kit, when combined with a base, forms the E-Gel® Imager System, which is a personal imaging system for documenting and analyzing agarose gels and E-Gel® cassettes. Each E-Gel® Camera Hood Kit includes a compact digital camera mounted in a sleek hood, a universal filter, power and USB cables, and two powerful software applications. There are three bases available for purchase that will work with the E-Gel® Camera Hood Kit: a UV Light Base, a Blue Light Base, and an E-Gel® Adaptor Base. In any of these three configurations, the E-Gel® Imager system provides a small and lig...
Catalog: 4466601
Size: 1 kit

InVision™ His-Tag In-Gel Stain

Category: Products & Services > Labeling & Detection Products > Gel & Membrane Stains

InVision™ His-tag In-gel Stain is a ready-to-use, fast, sensitive and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis. Using InVision™ His-tag In-gel Stain you'll: Stain proteins fused to an oligohistidine sequence Eliminate western blotting-detect His-tagged proteins directly in the gel Detect nanogram levels of protein bands with a UV transilluminator, equipped with a standard camera, or a laser-based scanner Visualize results in under 3 hours Convenienty provided as one ready-to-use solution, InVision™ His-tag ...
Catalog: LC6030
Size: 500 ml

NuPAGE® Novex® 3-8% Tris-Acetate Gels, 1.5 mm, 15 well

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Protein Gels > Tris-Acetate Gels

NuPAGE® Novex® 3–8% Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex® Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Formulation NuPAGE® Novex® Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended buffers Run NuPAGE® Novex® Tris-...
Catalog: EA03785BOX
Size: 1 box

Gel Loading Buffer II (Denaturing PAGE)

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Loading Buffers

A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue. Supplied in one 1.4 mL tube. All Ambion® Gel Loading Solutions are rigorously tested for non-specific endonuclease activity, exonuclease activity, RNase activity, and for functionality. These are the same solutions we use in-house and in our kits.
Catalog: AM8546G
Size: 1 ml

iBlot® Gel Transfer Device

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Blotting Systems

The iBlot® Gel Transfer Device performs Western blotting transfer efficiently and reliably, typically in 7 minutes or less without the need for liquid buffers. The iBlot® Gel Transfer Device is an integral part of the iBlot® Dry Blotting System, which consists of the transfer device and disposable blotting stacks which contain the buffers and the transfer membrane (nitrocellulose or PVDF). The iBlot® Dry Blotting System is for dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels, electroblotting of nucleic acids from agarose gels and TBE polyacrylamide gels, and performing Weste...
Catalog: IB1001
Size: 1 each

NuPAGE® Novex® 3-8% Tris-Acetate Gels, 1.0 mm, 2D well

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Protein Gels > 2-D Gels

NuPAGE® Novex® 3–8% Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex® Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Formulation NuPAGE® Novex® Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended buffers Run NuPAGE® Novex® Tris-...
Catalog: EA0376BOX
Size: 1 box

NuPAGE® Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Protein Gel Buffers > Protein Gel Sample Buffers

NuPAGE® Novex 3-8% Tris-Acetate Midi-Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. NuPAGE® Novex Tris-Acetate Midi-Gels (8 x 13 cm) can run up to 26 samples. The NuPAGE Midi-Gels run in the XCell4 Surelock™ Midi-Cell and, with a Midi Gel Adapter, can run in a Bio-Rad Criterion Cell. Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity,...
Catalog: LA0050
Size: 1 kit

Midi-Gel Adapters

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Other Gel Electrophoresis Accessories

The Midi Gel Adapter is designed for use with the Novex® Midi Gel Cassette (NuPAGE® or Tris-Glycine Gels) to perform electrophoresis using a BioRad Criterion™ Cell. The Midi Gel Adapter is also supplied with the Novex® Midi Gels with Adapters Kit.
Catalog: WA0999
Size: 10⁄box

NuPAGE® Novex® 7% Tris-Acetate Gels, 1.0 mm, 12 well

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Protein Gels > Tris-Acetate Gels

NuPAGE® Novex® 7% Tris-Acetate Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex® Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than with the Tris-Glycine gel system. Formulation NuPAGE® Novex® Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS. Recommended buffers Run NuPAGE® Novex® Tris-Ac...
Catalog: EA03552BOX
Size: 1 box

Pro-Q® Diamond Phosphoprotein Gel Destaining Solution, 1 L

Category: Products & Services > Labeling & Detection Products > Gel & Membrane Stains

Pro-Q Diamond phosphoprotein gel stain provides a convenient method for selectively staining phosphoproteins in acrylamide gels, without the need for blotting or phosphoprotein specific antibodies and Western blot analysis. Preparation of the Pro-Q Diamond phosphoprotein gel destaining solution is described in the gel staining protocol, but we also provide the detaining solution premixed for your convenience.
Catalog: P-33310
Size: 1 L

NuPAGE® Novex® 4-12% Bis-Tris ZOOM® Gels, IPG Well 1.0 mm, IPG Well

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Protein Gels > 2-D Gels

NuPAGE® Novex Bis-Tris Gels provide the best separation and resolution of small- to medium-sized proteins by utilizing a neutral pH environment which minimizes protein modifications. These high-performance gels have a one-year shelf life when stored at +4°C to 25°C, eliminating the waste of throwing out expired gels. Use NuPAGE® Novex Bis-Tris Gels for protein sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. The NuPAGE® System also provides the most efficient means for transferring proteins to membranes for subsequent analysis. Formulation: The NuPAGE...
Catalog: NP0330BOX
Size: 1 box

Pro-Q® Emerald 300 Lipopolysaccharide Gel Stain Kit

Category: Products & Services > Labeling & Detection Products > Gel & Membrane Stains

Molecular Probes' proprietary Pro-Q Emerald 300 and Pro-Q Emerald 488 Glycoprotein Stain Kits provide the most advanced technology available for detection of glycoproteins in gels and on blots. Gel staining is rapid and very sensitive. In less than three hours, it is possible to detect as little as 300 pg of glycoprotein per band, depending on the degree of glycosylation, making these stains at least 50-fold more sensitive than standard fuchsin staining.
Catalog: P-20495
Size: 1 kit

8% TBE Gels 1.0 mm, 10 Well

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Polyacrylamide Gels

Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex® DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex® TBE and DNA Retardation Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide,...
Catalog: EC6215BOX
Size: 1 box

6% TBE Gels 1.0 mm, 15 Well

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Nucleic Acid Gels > Polyacrylamide Gels

Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex® DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex® TBE and DNA Retardation Gels are designed to run on the XCell SureLock ™ Mini-Cell. Formulation: Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide,...
Catalog: EC62655BOX
Size: 1 box

10X BlueJuice™ Gel Loading Buffer

Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Nucleic Acid Gel Loading Buffers

BlueJuice™ Gel Loading Buffer is designed for easy loading and tracking of DNA samples in agarose or native polyacrylamide gels.
Catalog: 10816-015
Size: 3 × 1 ml

NativePAGE™ Novex® 4-16% Bis-Tris Gels 1.0 mm, 15 Well

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Protein Gels > Bis-Tris Gels

The NativePAGE™ Novex® Bis-Tris Gel System is a pre-cast polyacrylamide mini gel system that provides a sensitive and high-resolution method for analysis of native membrane protein complexes, native soluble proteins, molecular mass estimations, and assessment of purity of native proteins. It is based on the blue native polyacrylamide gel electrophoresis technique (BN PAGE) developed by Schagger and von Jagow (1-3). With the NativePAGE™ Novex® Bis-Tris Gel System, youll: • Simplify resolution of large proteins (15-10,000 kDa) • Analyze membrane-protein complexes in their native conformations • ...
Catalog: BN1004BOX
Size: 1 box

E-Gel® Safe Imager™ Real-Time Transilluminator

Category: Products & Services > Instruments, Software, & Accessories > Gel Boxes, Gel Imagers, Power Supplies & Accessories > Gel Imagers & Digital Electrophoresis Systems

The E-Gel® Safe Imager™ Real-Time Transilluminator allows you to watch migration of DNA in real-time in E-Gels containing SYBR® Safe. It offers: • Real-Time monitoring of DNA migration • Improved safety for the user • Enhanced cloning efficiency of retrieved DNA Real-Time Transilluminator Designed for Safety and Cloning The E-Gel® Safe Imager™ Real-Time Transilluminator is designed to fit perfectly beneath the E-Gel® iBase™ Power System and is intended for use with E-Gels containing SYBR® Safe. Blue light emitted by the instrument is ideal for viewing DNA that is stained with SYBR® Safe. Unlik...
Catalog: G6500
Size: 1 each

Novex® 8% Tris-Glycine Midi Gels, 12+2 well

Category: Products & Services > Gels, Fractionation Strips, & Membranes > Pre-Cast Protein Gels > Tris-Glycine Gels

Novex® 8% Tris-Glycine Midi Gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis. Novex® Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands. Each box contains 10 gels. Features of these gels: • Individually packaged gels for optimal experiment design • Compatible with most protein standards • Usable for native and denaturing protein assays Choose the right gel format for your experiments Novex® Tris-Glycine gels come in a variety of fixed concentrations from 4% to 18%, as well as gradients with ranges of 4–12%, 4–...
Catalog: WT0081BOX
Size: 1 box