Gateway Protein Expression Kits
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Catalog: K101-01
Size: 20 reactions
Size: 20 reactions
The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Catalog: K100-01
Size: 20 reactions
Size: 20 reactions
The FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein. Free your lab from the time-consuming, labor-intensive process of seeding, feeding, passing, and maintaining multiple flasks of adherent cells (Figure 1). The FreeStyle™ 293 Expression System combines GIBCO® FreeStyle™ 293 Expression Medium with 293fectin™, a cationic-lipid formulation designed to transfect suspension FreeStyle™ 293-F cells at high efficiency. FreeStyle™ 293-F cells and 293fectin™ are available as part of the Expression System or sep...
Catalog: K9000-01
Size: 1 kit
Size: 1 kit
The Champion™ pET SUMO Expression System produces the highest levels of soluble protein in E. coli . It utilizes a small ubiquitin-related modifier (SUMO) fusion, belonging to the growing family of ubiquitin-related proteins, to enhance the solubility of expressed fusion proteins. In contrast to ubiquitin, SUMO is involved in the stabilization and localization of proteins in vivo . After expression, the 11 kd SUMO moiety can be cleaved by the highly specific and active SUMO (ULP-1) protease at the carboxyl terminal, producing a native protein*. The Champion™ pET SUMO Protein and Peptide Expres...
Catalog: K300-01
Size: 20 reactions
Size: 20 reactions
The PichiaPink™ Secreted Protein Expression Kit is a new recombinant protein expression system based on the yeast Pichia pastoris . The PichiaPink™ Secreted Protein Expression Kit gives you convenient and cost-efficient bioproduction from shake flask to 1000-liter production of your recombinant protein. The PichiaPink™ Secreted Protein Expression Kit comes with our new Pichia strains, the pPINK-HC and pPINK-LC vectors, and secretion signal sequences for production of your protein. • Get started with secreted protein production in Pichia pastoris . • Screen your Pichia transformants rapidly by ...
Catalog: A11151
Size: 1 kit
Size: 1 kit
Novex® Tris-Glycine Express Kits are a convenient and cost-effective way to obtain small quantities of pre-cast tris-glycine gels and pre-stained protein standard. Each kit includes: • 5 Novex® 4-20% Tris-Glycine Mini Gels, 1.0 mm, 12 well • 50 µl of SeeBlue® Pre-Stained Standard Novex® Tris-Glycine Mini Gels Novex® Tris-Glycine Mini Gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands. • Individually packaged gels for optimal experiment design • Compatibl...
Catalog: EC60252BX5
Size: 5 gels
Size: 5 gels
The ViraPower™ HiPerform™ Promoterless Gateway® Vector Kit contains the MultiSite Gateway®-adapted ViraPower™ HiPerform™ promoterless lentiviral expression vector, pLenti6.4⁄R4R2⁄V5-DEST™ for easy recombination-based cloning and lentiviral-based high-level expression of a target gene from any promoter of choice in dividing and non-dividing mammalian cells. The pLenti6.4⁄R4R2⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integras...
Catalog: A11146
Size: 20 reactions
Size: 20 reactions
Invitrogen™ 's pLenti6.3⁄V5-DEST™ Gateway® Vector Kit is part of our ViraPower™ HiPerform™ Lentiviral Gateway® Expression Kit (catalog K5330-00). The pLenti6.3⁄V5-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5-DEST™ for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Poly...
Catalog: V533-06
Size: 1 kit
Size: 1 kit
The NativePure™ Mammalian Affinity Purification Kit provides an easy-to-use, efficient method for purifying native protein complexes. NativePure™ pcDNA™ Gateway® vectors (Figure 1) are designed to allow expression of biotinylated recombinant fusion proteins in mammalian cells through N- or C- terminal fusion of your recombinant protein of interest to the capTEV™ Tag. The capTEV™ Tag consists of a BioEase™ in vivo biotinylation peptide, two Tobacco Etch Virus (TEV) protease recognition sites, and a 6XHis tag. The BioEase™ tag, a 72-amino acid sequence from K. pneumoniae , directs in vivo biotin...
Catalog: BN3002
Size: 1 kit
Size: 1 kit
Gateway Protein Expression Kits
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