gateway cdnas
The Gateway® Vector Conversion System is designed to convert any vector into a Gateway® destination vector using restriction endonucleases and ligase. A Gateway® cassette containing att R recombination sites flanking a ccd B gene (1) and a chloramphenicol-resistance gene are blunt-end cloned into the multiple cloning site of any vector. ccdB Survival 2 competent cells allow propagation of the Gateway® vectors containing the ccd B gene. The Gateway® Vector Conversion System offers: • Conversion of any expression vector into a Gateway® destination vector • Three cassettes to construct a destinat...
Catalog: 11828-029
Size: 1 kit
Size: 1 kit
The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. This particular kit also includes 2 tubes of RNase inhibitor (100 µL each). The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is i...
Catalog: 4374967
Size: 1000 reactions
Size: 1000 reactions
The SuperScript® Double-Stranded cDNA Synthesis Kit contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality double-stranded cDNA from total RNA or poly A + selected RNA (mRNA). The advanced capabilities of SuperScript® II Reverse Transcriptase, with its reduced RNase H activity, maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis. Features of the SuperScript® Double-Stranded cDNA Synthesis Kit include: • Reliability —Each buffer, reagent, and enzyme in the kit is of the same high quality you expect from Life Technologie...
Catalog: 11917-010
Size: 10 reactions
Size: 10 reactions
The High Capacity RNA-to-cDNA™ Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix, and other PCR enzymes. Features of this kit: • Fast —short reaction time (typically 30–60 min) • Convenient —easy workflow with few pipetting steps (2 tubes) • Reliable —reverse transcription of both abundant and limited targets • Flexible —optimized 2-step protocol, enabling multiple PCR reactions from a single reverse transcription reaction Streamline your cDNA synthesis while maintaining detection sensitivit...
Catalog: 4387406
Size: 50 reactions
Size: 50 reactions
The NCode™ VILO™ miRNA cDNA Synthesis Kit is part of a novel quantitative assay that provides flexibility, sensitivity, and specificity when quantitating miRNA expression. The system combines a carefully optimized polyadenylation reaction with the market-leading reverse transcriptase, SuperScript® III RT, in a universal first-strand cDNA synthesis reaction. Tried and tested SuperScript® III Reverse Transcriptase is formulated in an enhanced buffer system to provide you with the most reliable firststrand synthesis and higher cDNA yields, with sensitivity and accurate quantitation from 10pg to 1...
Catalog: A11193-050
Size: 50 reactions
Size: 50 reactions
In addition to the previously listed Gene Pool™ cDNA, the following selection of cDNA from adult human normal, tumor, or disease tissues as well as human fetal normal tissue is available. See Gene Pool™ cDNA for description and storage information. All Special Order Products are subject to tissue/tumor availability. Please call for information. Allow up to two weeks for delivery.
Catalog: D8880-01
Size: 20 reactions
Size: 20 reactions
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 11809-019
Size: 6 µg
Size: 6 µg
The ViraPower™ HiPerform™ Promoterless Gateway® Vector Kit contains the MultiSite Gateway®-adapted ViraPower™ HiPerform™ promoterless lentiviral expression vector, pLenti6.4⁄R4R2⁄V5-DEST™ for easy recombination-based cloning and lentiviral-based high-level expression of a target gene from any promoter of choice in dividing and non-dividing mammalian cells. The pLenti6.4⁄R4R2⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integras...
Catalog: A11146
Size: 20 reactions
Size: 20 reactions
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 11801-016
Size: 6 µg
Size: 6 µg
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 11806-015
Size: 6 µg
Size: 6 µg
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 11802-014
Size: 6 µg
Size: 6 µg
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 11812-013
Size: 6 µg
Size: 6 µg
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: V6020-20
Size: 6 µg
Size: 6 µg
Invitrogen™ 's pLenti6.3⁄V5-DEST™ Gateway® Vector Kit is part of our ViraPower™ HiPerform™ Lentiviral Gateway® Expression Kit (catalog K5330-00). The pLenti6.3⁄V5-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5-DEST™ for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Poly...
Catalog: V533-06
Size: 1 kit
Size: 1 kit
The NativePure™ Mammalian Affinity Purification Kit provides an easy-to-use, efficient method for purifying native protein complexes. NativePure™ pcDNA™ Gateway® vectors (Figure 1) are designed to allow expression of biotinylated recombinant fusion proteins in mammalian cells through N- or C- terminal fusion of your recombinant protein of interest to the capTEV™ Tag. The capTEV™ Tag consists of a BioEase™ in vivo biotinylation peptide, two Tobacco Etch Virus (TEV) protease recognition sites, and a 6XHis tag. The BioEase™ tag, a 72-amino acid sequence from K. pneumoniae , directs in vivo biotin...
Catalog: BN3002
Size: 1 kit
Size: 1 kit
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 11804-010
Size: 6 µg
Size: 6 µg
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: V492-20
Size: 6 µg
Size: 6 µg
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 12302-014
Size: 6 µg
Size: 6 µg
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Catalog: 12301-016
Size: 6 µg
Size: 6 µg
The pLenti6.2⁄V5-DEST™ Gateway® Vector is a Gateway®-adapted ViraPower™ II lentiviral expression vector for lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of the target gene and the PGK promoter for driving long-term, persistent expression of the Blasticidin stable selection marker. Advantages • Stable expression • Long-term experiments • High-throughput screening • Accurate titer of functional virus (using Blasticidin method) • Flexible and versatile Gateway® recombination cloning t...
Catalog: V368-20
Size: 6 µg
Size: 6 µg
gateway cdnas
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