Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
Mouse anti-Galectin-1; Monoclonal antibody specific to Human, mouse, swine, rat, ovine, hamster, bovine Galectin-1.This antibody is validated for use in Western Blot, Immunocytochemistry/ Immunofluorescence.
Catalog: 437400
Size: 100 µg
Category: Products & Services > Kits & Assays > Cell Analysis Kits > Apoptosis Kits
The Vybrant Apoptosis Assay Kit #4 detects apoptosis on the basis of changes that occur in the permeability of cell membranes. This kit contains ready-to-use solutions of both YO-PRO-1 and propidium iodide nucleic acid stains. YO-PRO-1 stain selectively passes through the plasma membranes of apoptotic cells and labels them with moderate green fluorescence. Necrotic cells are stained red-fluorescent with propidium iodide.
Catalog: V13243
Size: 1 kit
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
This purified mouse anti-NY-ESO-1 monoclonal antibody recognizes human NY-ESO-1. NY-ESO-1 is a member of the cancer/testis (CT) family of antigens, which are expressed in a range of malignant neoplasmas such as melanoma and carcinomas of the breast, ovary and lung, but not in normal tissue except in testis. NY-ESO-1 is a classic CT antigen discovered during a SEREX analysis of esophageal cancer. It is a 22–23 kDa hydrophobic protein. Validated applications for anti-NY-ESO-1 are western blotting and immunohistochemistry (paraffin). Antibody Specifications: • Applications: Validated applications...
Catalog: 35-6200
Size: 100 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The CD90/Thy-1 (clone G7) R-PE (phycoerythrin) conjugate is a monoclonal rat antibody that recognizes the Thy-1.1 and Thy-1.2 alloantigens (Thy-1 epitope region A). R-PE is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thereby affording exceptional quantum yields and molar extinction coefficients. The use of PE in antibody conjugates provides probes with greatly enhanced detectability. Antibody Specifications Description: CD90/Thy-1, Rat IgG 2c monoclonal Label: R-PE (phycoer...
Catalog: A14726
Size: 25 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
This is a purified rabbit anti-human Lipocalin-1 oligoclonal antibody (oAb) that can be used in validated applications such as western blotting (WB), immunocytochemistry (ICC), and indirect ELISA. ABfinity™ antibody characteristics include: • APPLICATIONS: This Lipocalin-1 Oligoclonal Antibody, ABfinity™ Recombinant has been validated for use in western blotting, immunocytochemistry, and indirect ELISA. • HOST SPECIES AND ISOTYPE: This is a recombinant rabbit oligoclonal IgG against human Lipocalin-1. • CLONE ID OF OLIGOCLONAL (oAb): The oligoclonal antibody clone is 14HCLC. • REACTIVITY: Alon...
Catalog: 710406
Size: 100 µg
Category: Products & Services > Antibodies & Secondary Detection Reagents > Primary Antibodies > Protein Specific Primary Antibodies
The BP-1/LY-51/6C3, clone FG35.4, FITC dye conjugate is a rat monoclonal antibody that is reactive to mouse BP-1/Ly-51/6C3 (aminopeptidase A, E.C.3.4.11.7, glutamylaminopeptidase). Commonly used, the fluorescein conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Antibody Specifications Description: BP-1/LY-51, Rat IgG1κ monoclonal Label: FITC Validated Application: Flow Cytometry Species Reactivity: Mouse Ex/Em and laser configuration: 488/519 nm, blue laser Clone ID: FG35.4 Leukocyte Workshop Status: NA Regulatory Status: RUO
Catalog: A15405
Size: 0.1 mg
Category: Products & Services > Buffers & Chemicals > General Buffers > Tris & TE Buffers
Ambion® Molecular biology grade, 1M Tris, pH 7.0 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contamina...
Catalog: AM9851
Size: 500 ml
Category: Products & Services > Cell Culture & Transfection Reagents > Cell Dissociation Reagents > Trypsin
Gibco® Trypsin 1:250 powder is an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength it is widely used for cell dissociation, during routine cell culture passaging, and primary tissue dissociation. Life Technologies offers a variety of Gibco® Trypsin and animal origin free TrypLE ™ that feature: Quality Testing Documented Traceability Dual-site cGMP Manufacturing Quality Testing Gibco® Trypsin 1:250 is tested for endotoxin, mycoplasma, bacterial, fungal and viral contamination. Other quality tests include multiple activity assays, ash analysis and mois...
Catalog: 27250-018
Size: 100 g
Category: Products & Services > Kits & Assays > Sequencing Kits & Reagents > Library Preparation Kits for Sequencing
The Ion Xpress™ Barcode Adapters 1-16 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Ba...
Catalog: 4471250
Size: 1 kit
Category: Products & Services > Kits & Assays > Real-Time PCR & Endpoint RT-PCR Kits > qPCR Kits
Applied Biosystems® TaqMan® Universal PCR Master Mix, No AmpErase® UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications. Applied Biosystems® reagents have been validated with TaqMan® Assays and Applied Biosystems® real-time systems to ensure sensitive, accurate, and reliable performance every time. • Contains AmpliTaq Gold® DNA polymerase to provide a better yield and a more robust 5' nuclease assay than AmpliTaq® DNA polymerase • Includes a passive internal reference based on proprietary ROX™ dye, for superb precision on Applied Biosystems® re...
Catalog: 4326614
Size: 2000 reactions
Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains
The cell-impermeant viability indicator ethdium homodimer-1 (EthD-1) is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (excitation/emission maxima ~528/617).
Catalog: E1169
Size: 1 mg
Category: Products & Services > Buffers & Chemicals > Electrophoresis Reagents & Buffers > Acrylamide & Polymerizing Agents
Ambion® Acrylamide/Bis 19:1 is a 40% (w/v) solution of acrylamide (38%) and bis-acrylamide (2%) ideal for use in ribonuclease protection assay, sequencing gels, and sizing DNA or RNA fragments. Supplied in one bottle containing 500 mL. The solution is provided in a ready-to-use form, reducing the dust, inhalation, and contact hazard associated with weighing and preparing acrylamide and bis-acrylamide powders and solutions. Concentrations are carefully controlled and all solutions are micro-filtered prior to packaging. All Ambion® acrylamides are rigorously tested for contaminating non-specific...
Catalog: AM9022
Size: 500 ml
Category: Products & Services > Buffers & Chemicals > General Chemicals > Other General Chemicals
UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl. When mixtures are extracted with Phenol:Chloroform:Isoamyl Alcohol, proteins are denatured and collected in the organic phase or at the interphase, while nucleic acids remain in the aqueous phase. UltraPure™ Phenol:Chloroform:lsoamyl Alcohol contains no preservatives. It is packaged under an inert gas in shatter-resistant, plastic-coated amber bottles. Performance and Quality Te...
Catalog: 15593-049
Size: 400 ml
Category: Products & Services > Kits & Assays > Genotyping Kits > SNP Genotyping Kits
The TaqMan® GTXpress™ Master Mix delivers accurate genotyping results with robust performance in less than 50 min. Applied Biosystems® TaqMan® GTXpress™ Master Mix (2X) combines AmpliTaq® Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format. The 1 mL pack includes reagents sufficient to perform 400 PCR reactions (5 µL/reaction). • Compatible with both Fast and Standard mode thermal cycling conditions • Pre-mixed components stored at 2°–8°C significantly reduce assay setup time • Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays •...
Catalog: 4403311
Size: 400 reactions
Category: Products & Services > Buffers & Chemicals > Cell Lysis & Organelle Isolation Reagents
These 2 buffers are to be used only as a complement to the SuperScript® III Platinum® CellsDirect Kits when large number of samples are processed. See manual of SuperScript® III Platinum® CellsDirect Kits for protocol recommendations.
Catalog: 11739-010
Size: 1 kit
Category: Products & Services > Antibodies & Secondary Detection Reagents > Secondary Antibodies
The Alexa Fluor® 488 goat anti-mouse IgG 1 is labeled with our exceptionally bright, photostable Alexa Fluor® 488 dye (which is spectrally similar to fluorescein) and is prepared from affinity-purified antibodies that react with the Fc portion of the heavy chain of mouse IgG 1 . To minimize crossreactivity, the antibody has been adsorbed against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins and mouse isotypes IgG 2a , IgG 2b and IgG 3 prior to labeling.
Catalog: A-21121
Size: 250 µl
Category: Products & Services > Controls, Standards & Ladders > Synthetic RNAi Controls > RNAi Negative Controls
Ambion® In Vivo siRNA Controls • Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts • Positive Control siRNAs functionally tested in several common cell lines • Negative Controls functionally proven to have minimal effects on cell proliferation and viability • Include Silencer® Select modifications for enhanced specificity or Ambion® In Vivo modifications for increased stability • For use in human, mouse, and rat cells What are Ambion® In Vivo siRNAs? Ambion® In Vivo siRNAs are designed using the proven Silencer ® Select algorithm and incorpora...
Catalog: 4457287
Size: 5 nmol
Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains
DASPMI (4-Di-1-ASP) is a styryl dye stains mitochondria of live cells. This dye has a large fluorescence Stokes shift and is taken up relatively slowly as a function of membrane potential.
Category: Products & Services > Labeling & Detection Products > Labels & Labeling Kits > Cell, Tissue, & In Vivo Labeling Reagents
Molecular Probes® CellTracker™ fluorescent probes freely pass through cell membranes and are converted to cell-impermeant reaction products. The cell-impermeant form is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CellTracker™ probes are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, the staining pattern can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate s...
Catalog: C2925
Size: 1 mg
Category: Products & Services > Buffers & Chemicals > Water
Ambion® Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving. Because it is not DEPC treated, it is recommended for applications for which residual DEPC might prove detrimental (e.g. oocyte microinjection). This nuclease-free water is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
Catalog: AM9930
Size: 1 × 500 ml