Free Calcium
Gibco® Protein Free Hybridoma Medium II (PFHM II) is a serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® PFHM II features: • Ability to support suspension and stationary hybridomas and myelomas • Complete, ready-to-use formulation • Serum-free, protein-free formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-depende...
Catalog: 23600042
Size: 1 x 10L
Size: 1 x 10L
Ambion® TURBO DNA- free ™ contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. The kit includes sufficient reagents for 50 rxns. Features of Ambion® TURBO DNA- free ™ include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can plague RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The Best Method for Genomic DNA Removal Prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNa...
Catalog: AM1907
Size: 50 reactions
Size: 50 reactions
Ambion® Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving. Because it is not DEPC treated, it is recommended for applications for which residual DEPC might prove detrimental (e.g. oocyte microinjection). This nuclease-free water is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
Catalog: AM9930
Size: 1 × 500 ml
Size: 1 × 500 ml
Intracellular calibration of the fluorescence response of cytosolic pH indicators is typically performed using the K + /H + ionophore nigericin , which causes equilibration of intracellular and extracellular pH in the presence of a depolarizing concentration of extracellular K + .
Catalog: N-1495
Size: 10 mg
Size: 10 mg
Gibco® Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Phosphate Buffered Saline (PBS). Gibco® Cell Dissociation Buffer is suitable for gentle dissociation of mammalian cells for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry. Gibco® Cell Dissociation Buffer works well with lightly adherent cell lines such as HeLa and NIH 3T3, but is not recommended for routine passage of strongly adherent cells. The dis...
Catalog: 13151-014
Size: 100 ml
Size: 100 ml
High-Yield Lyse is a premixed, fixative-free erythrocyte lysing solution that can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with Invitrogen's fluorochrome-conjugated monoclonal antibodies.
Catalog: HYL-250
Size: 500 ml
Size: 500 ml
Invitrogen's Calcium Phosphate Transfection Kit provides high-quality reagents to enable the introduction of DNA into eukaryotic cells via calcium phosphate co-precipitation.
Catalog: K2780-01
Size: 75 reactions
Size: 75 reactions
The Calcium Calibration Buffer Kits are designed to help determine the dissociation constant (K d ) of fluorescent Ca 2+ indicators using a fluorometer or quantitative imaging system. The Kd of an indicator can be calculated from a plot generated by scanning the excitation or emission of the indicator in the presence of eleven different Ca 2+ concentrations. Calcium Calibration Kit #1 contains 50 mL of 10 mM K 2 EGTA and 50 mL of 10 mM CaEGTA. Both solutions contain 100 mM KCl and 30 mM MOPS, pH 7.2 and are prepared in deionized water. These stock solutions can be blended to make buffers havin...
Catalog: C-3008MP
Size: 1 kit
Size: 1 kit
The RNase-free TE buffer concentrate, 20X TE buffer , which is essential to the success of the RiboGreen® RNA quantitation assays (R11491, R37302) is available separately from the kits to extend the number of low-concentration assays.
Catalog: T11493
Size: 100 ml
Size: 100 ml
Ambion® RNase-free Microfuge Tubes are guaranteed RNase- and DNase-free and have a positive seal design that withstands –90° to 120°C. They are marked with convenient graduations. 1.5 mL tubes are provided in a box of 500. Each lot of tubes is certified RNAse- and DNase-free.
Catalog: AM12400
Size: 500 tubes
Size: 500 tubes
Ambion® IPTG (Isopropyl-beta-D-thiogalactoside) is an inducer of the lac operon and is frequently used in conjunction with X-GAL to determine the lac phenotype of recombinant plasmids or phage. IPTG is also used to induce the expression of cloned genes that are under control of the lac operon. Supplied in one bottle containing 5 g.
Catalog: AM9464
Size: 5 g
Size: 5 g
Ambion® Non-Stick RNase-free Microfuge Tubes have a low binding surface and are guaranteed RNase- and DNase-free. They have a positive seal design that withstands –90° to 120°C and are marked with convenient graduations. 1.5 mL tubes are provided in a box of 250. Each lot of tubes is certified RNAse- and DNase-free.
Catalog: AM12450
Size: 250 tubes
Size: 250 tubes
Ambion® TURBO DNA- free ™ contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. The kit includes sufficient reagents for 50 rxns. Features of Ambion® TURBO DNA- free ™ include: • Hyperactive TURBO™ DNase is a catalytically superior enzyme compared to wild type DNase I • Removes trace quantities of DNA that can plague RT-PCR • Reagent included to completely remove DNase without phenol treatment or heating The Best Method for Genomic DNA Removal Prior to RT-PCR TURBO™ DNase is a recombinant, engineered form of DNa...
Catalog: AM1907M
Size: 50 rxns w⁄ Manual
Size: 50 rxns w⁄ Manual
UltraPure™ DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. It is 0.1-µm membrane-filtered and tested for DNase, RNase, and protease activity.
Catalog: 10977035
Size: 500 ml
Size: 500 ml
Human Endothelial-SFM is a basal serum-free growth medium developed to support the isolation and expansion of human umbilical venous and arterial endothelial cells (1–4). Medium requires supplementation with bFGF (20 ng/ml), EGF (10 ng/ml), and human plasma fibronectin (10 µg/ml). Contains L-glutamine.
Catalog: 11111-044
Size: 500 ml
Size: 500 ml
Gibco® CD Hybridoma Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features: • Ability to support suspension and stationary hybridomas and myelomas • Formulated without L-glutamine for stability • Animal origin-free, protein-free and chemically defined formulation Ability to support suspension and stationary hybridomas and myelomas Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® CD Hybridoma Medi...
Catalog: 11279-023
Size: 1,000 ml
Size: 1,000 ml
FreeStyle™ 293 Expression Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required. Gibco® FreeStyle™ 293 Expression Medium features: • Complete and ready-to-use format containing GlutaMAX™ supplement • Ability to support suspension growth and transfection of 293-F cells • Animal origin-free, protein-free and chemically defined formulation • Easy use as part of the FreeStyle™ 293 ...
Catalog: 12338-018
Size: 1000 ml
Size: 1000 ml
Defined Keratinocyte-SFM is a serum-free medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers (1,2). The growth-promoting activity of BPE has been replaced by the inclusion of growth promoting agents which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration ( Note: This formulation contains s...
Catalog: 10744-019
Size: 500 ml
Size: 500 ml
VP-SFM AGT™ is a serum free, ultra low protein medium (5 µg/ml) in a new easy-to-use granular format. VP-SFM AGT™ is designed for the growth of VERO cells for virus production. It is also suitable for the growth of COS-7, MDCK, BHK-21 and Hep2 cells and for recombinant protein production.
Catalog: 12559-019
Size: 10 L
Size: 10 L
CD CHO medium is a protein-free, serum-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. With a proven track record of quality for more than 10 years, CD CHO medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates. When to Use CD CHO Medium • Your GS CHO cells are recommended to be grown in CD CHO medium • Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a reg...
Catalog: 10743-029
Size: 1,000 ml
Size: 1,000 ml
Free Calcium
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