fluorescence reader

RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit - one 96-Well Microplate

Category: Products & Services > Kits & Assays > Enzyme Detection & Activity Assays > Phosphatase Assay Kits

The RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensur...

Catalog: R33700
Size: 1 kit

Fluo-4, Pentapotassium Salt, cell impermeant

Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+ . Fluo-3 has been used to image the spatial dynamics of Ca 2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt ...

Catalog: F-14200
Size: 500 µg

AlamarBlue®

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

alamarBlue® is a redox indicator that yields a colorimetric change and a fluorescent signal in a response to a metabolic activity. It is a proven safe and non-toxic dye used for quantitative analysis of cell viability and cell proliferation, for cytokine bioassays and in vitro cytotoxicity studies. Applications: • Cell proliferation determinations —alamarBlue® is water soluble, stable in culture and non-toxic • Cell viability assays, including cytotoxicity —metabolic activity and dye generation changes in proportion to altered viability and cell damage • Cytokine assays —measure cytokine-induc...

Catalog: DAL1100
Size: 100 ml

lucigenin (bis- N -Methylacridinium Nitrate) - High Purity

Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores

Our highly purified lucigenin is a fluorescent Cl- indicator that has excitation/emission maxima of 368/505 nm and whose fluorescence is quenched in the presence of Cl-. The dye is useful for determining Cl- concentrations in liposomes, reconstituted membranes or in extracellular medium but is not useful for intracellular measurements.

Catalog: L6868
Size: 10 mg

SNARF®-1 Carboxylic Acid, Acetate, Succinimidyl Ester - Special Packaging

Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores

To permit simultaneous long-term tracing of mixed-cell populations using different fluorescent colors, we have developed a probe whose applications should be similar to those of CFDA SE (commonly called CFSE). Cells labeled with our SNARF-1 carboxylic acid, acetate, succinimidyl ester have orange-red fluorescence that can be easily distinguished from that of cells loaded with green-fluorescent tracers such as CFDA SE.

Catalog: S22801
Size: 10 × 50 µg