Fluorescence Cell

SYTO® 60 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains

The cell-permeant SYTO 60 red fluorescent nucleic acid stain exhibits bright, red fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340) to enable researchers to find the most appropriate red-fluorescent SYTO stain for their system.
Catalog: S11342
Size: 250 µl

SYTO® 82 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains

The cell-permeant SYTO 82 orange fluorescent nucleic acid stain exhibits bright, orange fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary between cell types, we offer the SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit (S-11360) to enable researchers to find the most appropriate orange-fluorescent SYTO stain for their system.
Catalog: S11363
Size: 250 µl

NucBlue® Fixed Cell ReadyProbes™ Reagent

Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains

DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA. With NucBlue® Fixed Cell ReadyProbes™ Reagent, we have formulated a high-purity form of this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Features of NucBlue® Fixed Cell ReadyProbes™ Reagent: Ready-to-use liquid DAPI formulation No need to dilute, weigh, or pipette Convenient dropper bottle—just use two drops per ml Stable at room temperature—keep handy at your scope or...
Catalog: R37606
Size: 1 kit

Vybrant® DiI Cell-Labeling Solution

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells. DiI is also available as a solid (D-282), as a paste (N-22880) or as crystals (D-3911).
Catalog: V-22885
Size: 1 ml

Vybrant® CM-DiI Cell-Labeling Solution

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

The Vybrant® CM-DiI cell-labeling solution is a dye delivery solution that can be added directly to normal culture media to uniformly label suspended or attached culture cells for use in cell-cell fusion, cellular adhesion and migration applications.
Catalog: V-22888
Size: 1 ml

Vybrant® DiD Cell-Labeling Solution

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

The Vybrant® DiD cell-labeling solution is a dye delivery solution that can be added directly to normal culture media to uniformly label suspended or attached culture cells for use in cell-cell fusion, cellular adhesion and migration applications.
Catalog: V-22887
Size: 1 ml

ER-Tracker™ Blue-White DPX, for live-cell imaging

Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains

ER-Tracker Blue-White DPX dye is a photostable probe that is selective for the endoplasmic reticulum (ER) in live cells. The cells can be aldehyde-fixed after staining with the dye but significant fluorescence signal will be lost. With excitation at ~374 nm, the environmentally-sensitive probe exhibits emission between 430 and 640 nm. A long-pass DAPI filter works best.
Catalog: E-12353
Size: 20 × 50 µl

ER-Tracker™ Red (BODIPY® TR Glibenclamide), for live-cell imaging

Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains

The ER-Tracker™ Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY® TR dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K + channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell typ...
Catalog: E34250
Size: 100 µg

Fluo-4, Pentapotassium Salt, cell impermeant

Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca 2+ . Fluo-3 has been used to image the spatial dynamics of Ca 2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt ...
Catalog: F-14200
Size: 500 µg

Fura-FF, Pentapotassium Salt, cell impermeant

Category: Products & Services > Labeling & Detection Products > Sensors & Indicators > Ion Indicators & Ionophores

Fura-FF pentapotassium salt is a fura-2 derivative that has Ca 2+ sensitivity similar to mag-fura-2, but is relatively insensitive to Mg 2+ . This water-soluble salt form is useful for intracellular loading by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent (I-14402).
Catalog: F14180
Size: 500 µg

LysoTracker® Deep Red

Category: Products & Services > Labeling & Detection Products > Cell Stains > Organelle Stains

LysoTracker® Deep Red is a deep red-fluorescent dye for labeling and tracking acidic organelles in live cells. Features of all LysoTracker® probes include: • High selectivity for acidic organelles • Effective labeling of live cells at nanomolar concentrations • Live-cell imaging • With excitation/emission of 647/668 nm, the same filter set can be used as used for Cy7 or MitoTracker® Deep Red Simple, Highly Specific, One-Step Staining and Tracking of Acidic Organelles The LysoTracker® probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH. This all...
Catalog: L12492
Size: 5 x 50 µl

ActinGreen™ 488 ReadyProbes™ Reagent

Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents

ActinGreen™ 488 ReadyProbes™ Reagent is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. Like other ReadyProbes™ reagents, the ActinGreen™ 488 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells. ActinGreen™ 488 ReadyProbes™ Reagent provides: • High-affinity staining of F-actin with superior specificity compared to antibody methods • Outstanding fluorescence performance (Ex/Em: 495/518 nm) • Ready-to-use liquid formulati...
Catalog: R37110
Size: 1 kit

Premo™ Autophagy Sensor RFP-p62 Kit

Category: Products & Services > Kits & Assays > Cell Analysis Kits > Autophagy Detection Kits

The Premo™ Autophagy Sensor RFP-p62 Kit combines the ability to monitor the induction or inhibition of autophagy, through the localization of the autophagy receptor p62 (also known as SQSTM1), with the high transduction efficiency and minimal toxicity of BacMam 2.0 technology. To perform an image-based analysis for autophagy, simply add the BacMam 2.0 RFP-p62 reagent to your mammalian cells, incubate overnight to ensure adequate protein expression, and then visualize using standard RFP (red fluorescent protein) settings. The RFP gene included in this chimera is mKate2. This monomeric fluoresce...
Catalog: P36241
Size: 1 kit

ActinRed™ 555 ReadyProbes™ Reagent

Category: Products & Services > Labeling & Detection Products > Cellular Structure Probes > Fluorescent Dye – Based Reagents

ActinRed™ 555 ReadyProbes™ Reagent is a selective, high-affinity F-actin probe conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). Like other ReadyProbes™ reagents, ActinRed™ 555 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells. ActinRed™ 555 ReadyProbes™ Reagent provides: • High-affinity staining of F-actin with superior specificity compared to antibody methods • Outstanding fluorescence performance (Ex/Em: 540/565 nm) • Ready-to-use liquid formulation in convenie...
Catalog: R37112
Size: 1 kit

Premo™ Autophagy Sensor GFP-p62 Kit

Category: Products & Services > Kits & Assays > Cell Analysis Kits > Autophagy Detection Kits

The Premo™ Autophagy Sensor GFP-p62 Kit combines the ability to monitor the induction or inhibition of autophagy, through the localization of the autophagy receptor p62 (also known as SQSTM1), with the high transduction efficiency and minimal toxicity of BacMam 2.0 technology. To perform an image-based analysis for autophagy, simply add the BacMam 2.0 GFP-p62 reagent to your mammalian cells, incubate overnight to ensure adequate protein expression, and then visualize using standard GFP (green fluorescent protein) settings. The GFP gene included in this chimera is TagGFP2, which has been demons...
Catalog: P36240
Size: 1 kit

Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B Kit

Category: Products & Services > Kits & Assays > Cell Analysis Kits > Autophagy Detection Kits

The Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B Kit combines the ability to monitor the various stages of autophagy (through LC3B protein localization) with the high transduction efficiency and minimal toxicity of BacMam 2.0 expression technology. To perform image-based analysis for autophagy, simply add the BacMam 2.0 RFP-GFP-LC3B reagent to your mammalian cells, incubate overnight to ensure maximum protein expression, and then visualize using standard GFP (green fluorescent protein) and RFP (red fluorescent protein) settings. This tandem RFP-GFP sensor capitalizes on the pH difference betwee...
Catalog: P36239
Size: 1 kit

NucGreen™ Dead 488 ReadyProbes™ Reagent

Category: Products & Services > Labeling & Detection Products > Cell Stains > Nucleic Acid Cell Stains

NucGreen™ Dead 488 ReadyProbes™ Reagent is a cell-impermeant stain that emits exceptionally bright, green fluorescence when bound to DNA. Cells that have lost plasma membrane integrity are stained within minutes, making this an extremely useful stain to estimate live/dead cell ratios and to measure cytotoxicity in kinetic live-cell assays. It is also suitable for staining nuclei in fixed cell preparations and tissue sections. Like other ReadyProbes™ reagents, NucGreen™ Dead 488 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and dr...
Catalog: R37109
Size: 1 kit

Alkaline Phosphatase Live Stain

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

Alkaline Phosphatase Live Stain is a stem cell imaging product that allows users to differentially stain pluripotent stem cells (PSCs). The dye is a cell-permeable fluorescent substrate for alkaline phosphatase (AP) that is non-toxic to cells, diffusing out over the course of two hours. The Alkaline Phosphatase Live Stain consists of 50 µL of a 500X fluorescein-based dye in DMSO, sufficient for staining two 24-well plates, four 6 cm dishes or two 10 cm dishes. This Alkaline Phosphatase Live Stain allows you to: • Identify pluripotent stem cells with a strong signal above background • Stain PSC...
Catalog: A14353
Size: 50 µl

4-Di-1-ASP (4-(4-(Dimethylamino)styryl) -N-Methylpyridinium Iodide)

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

DASPMI (4-Di-1-ASP) is a styryl dye stains mitochondria of live cells. This dye has a large fluorescence Stokes shift and is taken up relatively slowly as a function of membrane potential.
Catalog: D-288
Size: 1 g

AlamarBlue®

Category: Products & Services > Labeling & Detection Products > Cell Stains > Other Cell Stains

alamarBlue® is a redox indicator that yields a colorimetric change and a fluorescent signal in a response to a metabolic activity. It is a proven safe and non-toxic dye used for quantitative analysis of cell viability and cell proliferation, for cytokine bioassays and in vitro cytotoxicity studies. Applications: • Cell proliferation determinations —alamarBlue® is water soluble, stable in culture and non-toxic • Cell viability assays, including cytotoxicity —metabolic activity and dye generation changes in proportion to altered viability and cell damage • Cytokine assays —measure cytokine-induc...
Catalog: DAL1100
Size: 100 ml